Angiogenic properties of endometrial mesenchymal stromal cells in endothelial co-culture: an in vitro model of endometriosis

Canosa, Stefano; Moggio, Aldo; Brossa, Alessia; Pittatore, Giulia; Marchino, Gian Luigi; Leoncini, S.; Benedetto, Chiara; Revelli, Alberto; Bussolati, Benedetta

doi:10.1093/molehr/gax006

Cited by 19 publications

(27 citation statements)

References 48 publications

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“…Several markers for isolation of endometrial mesenchymal stem/stromal cells (emMSCs) have been used, including CD146, PDGFRβ, and SUSD2 [177]. These emMSCs have been isolated from eutopic and ectopic endometria in human biopsies [179,180,181,182,183,184]. Studies have shown that mesenchymal stem/stromal cells (MSCs) derived from endometriotic implants have higher expression levels of pluripotent octamer-binding transcription factor 4 (OCT4) and responded better to niche cytokines such as TGF-β to increase proliferation, migration, invasion, and angiogenic properties compared with MSCs derived from a eutopic endometrium or healthy control endometrial tissue [140,141,179,182].…”

Section: Cell-based Therapy For Endometriosis Treatmentmentioning

confidence: 99%

Chronic Niche Inflammation in Endometriosis-Associated Infertility: Current Understanding and Future Therapeutic Strategies

Lin

Chen

Chang

et al. 2018

IJMS

140

104

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Endometriosis is an estrogen-dependent inflammatory disease that affects up to 10% of women of reproductive age and accounts for up to 50% of female infertility cases. It has been highly associated with poorer outcomes of assisted reproductive technology (ART), including decreased oocyte retrieval, lower implantation, and pregnancy rates. A better understanding of the pathogenesis of endometriosis-associated infertility is crucial for improving infertility treatment outcomes. Current theories regarding how endometriosis reduces fertility include anatomical distortion, ovulatory dysfunction, and niche inflammation-associated peritoneal or implantation defects. This review will survey the latest evidence on the role of inflammatory niche in the peritoneal cavity, ovaries, and uterus of endometriosis patients. Nonhormone treatment strategies that target these inflammation processes are also included. Furthermore, mesenchymal stem cell-based therapies are highlighted for potential endometriosis treatment because of their immunomodulatory effects and tropism toward inflamed lesion foci. Potential applications of stem cell therapy in treatment of endometriosis-associated infertility in particular for safety and efficacy are discussed.

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Section: Cell-based Therapy For Endometriosis Treatmentmentioning

confidence: 99%

Chronic Niche Inflammation in Endometriosis-Associated Infertility: Current Understanding and Future Therapeutic Strategies

Lin

Chen

Chang

et al. 2018

IJMS

140

104

View full text Add to dashboard Cite

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“…Typically, a threshold of 40 IU/L is used 36 hours after hCG administration to ensure oocytes are collected at time of retrieval. Others have suggested a serum β-hCG level of 20 IU/L on the day of retrieval as the threshold to verify that the correct dose of hCG has been administered, since this is the serum level that will result in a positive urinary pregnancy test [13]. The level of serum β-hCG on the day of egg collection below which an attempted oocyte retrieval will result in an empty follicle is reported as <12 IU/L [9,14].…”

Section: Discussionmentioning

confidence: 99%

“…This is true, given that the serum β-hCG 39 hours after ovulation triggering in our case is bound to be ≤8 IU/L; however, we did not draw blood to ascertain the serum β-hCG level at the time of oocyte retrieval. It is known that the prevalence of FEFS ranges from 0.07 to 0.32% [13]. The significance of this can be expressed by calculating the number needed to treat.…”

Section: Discussionmentioning

confidence: 99%

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Successful Oocyte Retrieval, Fertilization, and Clinical Pregnancy with Low Serum β-hCG on the Day of Oocyte Collection: A Reappraisal of the Definition of the Empty Follicle Syndrome

Velde

Chatzicharalampous

Awonuga

2020

Case Reports in Obstetrics and Gynecology

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Objective. To describe a case of successful oocyte retrieval, fertilization and clinical pregnancy despite very low β-hCG level, twelve hours after ovulation trigger. Design. Case report. Setting. Academic medical center. Patient. A 38-year-old patient inadvertently administered 2,000 IU hCG for final oocyte maturation; serum hCG twelve hours later was 16 IU/L. Interventions. Effort to obtain and administer a booster dose of hCG over the next twenty-seven hours failed. Main Outcome. Successful oocyte retrieval. Results. Fourteen oocytes were retrieved of which twelve were in metaphase II and nine fertilized after intracytoplasmic sperm injection (ICSI). Of these, eight embryos survived to day 5 and were subjected to preimplantation genetic screening (PGS) by comparative genomic hybridization (CGH). Results were available the next day, three of the embryos were euploid and one was transferred on day 6. Pregnancy was confirmed twelve days later and currently the patient has an ongoing singleton intrauterine pregnancy. Conclusion. Reproductive Endocrinology and Infertility specialists should be aware that final oocyte maturation could occur following injection of a lower dose of hCG with excellent fertilization rate and embryo development.

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“…(Fig. 1C) (37), which can be used as a model for studying the angiogenesis of endometriosis in vitro.…”

Section: Human Primary Endometrial Epithelial Cells (Eecs) and Endomementioning

confidence: 99%

In‑vitro models of human endometriosis (Review)

Fan

2019

Exp Ther Med

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Endometriosis is one of the most common benign gynecological diseases in women of reproductive age worldwide. In past decades, a number of in-vitro models have been used to investigate the pathology and therapeutic methods for the treatment of endometriosis. The current review summarized the majority of currently available in-vitro models, which utilize a variety of cell or tissues types, including endometriotic cell lines, primary endometrial stromal cells, endometrial stem cells, endometrial explants, peritoneal explants and immune cells. These cells or tissues are cultured individually, co-cultured in 2D or 3D systems with various matrices or cultured in chicken chorioallantotic membranes and amniotic membranes culture systems. These models are able to represent one or more aspects of the process of endometriosis. These models are helpful and can be used to investigate the development of endometriosis and the underlying mechanisms of this disorder in detail, and help investigators select appropriate models for their experiments. Recently, the new concept of endometriosis as a fibrotic condition will lead research to investigate the differentiation of myofibroblasts and the development of fibrosis in endometriotic lesions, which will increase the development of novel models that can be used to investigate endometriotic fibrosis. Contents 1. Introduction 2. In-vitro models 3. Discussion In-vitro models of human endometriosis (Review)

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Angiogenic properties of endometrial mesenchymal stromal cells in endothelial co-culture: an in vitro model of endometriosis

Cited by 19 publications

References 48 publications

Chronic Niche Inflammation in Endometriosis-Associated Infertility: Current Understanding and Future Therapeutic Strategies

Chronic Niche Inflammation in Endometriosis-Associated Infertility: Current Understanding and Future Therapeutic Strategies

Successful Oocyte Retrieval, Fertilization, and Clinical Pregnancy with Low Serum β-hCG on the Day of Oocyte Collection: A Reappraisal of the Definition of the Empty Follicle Syndrome

In‑vitro models of human endometriosis (Review)

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