Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease

Høier, Birgitte; Walker, Meegan; Passos, Madla A.; Walker, Philip J.; Green, Anita; Bangsbo, Jens; Askew, Christopher D.; Hellsten, Ylva

doi:10.1152/japplphysiol.00979.2013

Cited by 54 publications

(67 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding concurs with our previous work showing that eNOS mRNA expression and protein content is similar between PAD patients and healthy older adults [34], suggesting that the capacity for NO production is similar and that removal of NO by ROS is likely responsible for group differences in NO availability. Indeed, PAD patients displayed significantly elevated amounts of muscle NADPH oxidase compared with healthy controls.…”

Section: A C C E P T E D Accepted Manuscriptsupporting

confidence: 93%

Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease

et al. 2016

View full text Add to dashboard Cite

show abstract

Section: A C C E P T E D Accepted Manuscriptsupporting

confidence: 93%

Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Previous findings of reduced muscle vascular endothelial growth factor protein content (26) and elevated thrombospondin-1 in the muscle (16) and muscle extracellular fluid (26) also provides some indication that the angiogenic potential of PAD-affected muscle may be impaired. However, the capillary-to-fiber ratio differed only nonsignificantly between the two groups, which is in contrast to other studies (16,45).…”

Section: Discussionmentioning

confidence: 89%

“…On a separate day, VL biopsies were taken from the 14 IC patients and the 10 control participants by a medical practitioner using a Bergstroem needle with suction (26,51). All biopsies were taken from the participants at rest within a 6-wk period.…”

Section: Methodsmentioning

confidence: 99%

Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

Baum

Torchetti

Malik

et al. 2016

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

(PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC patients and 10 age-matched controls, and peak power output (PPO) was determined for all participants using an incremental single-leg kneeextension protocol. Capillary density was lower (411 Ϯ 90 mm Ϫ2 vs. 506 Ϯ 95 mm Ϫ2 ; P Յ 0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543 Ϯ 82 nm vs. 423 Ϯ 97 nm; P Յ 0.01) and the volume density of mitochondria was lower (3.51 Ϯ 0.56% vs. 4.60 Ϯ 0.74%; P Յ 0.01) in the IC patients than the controls. In the IC patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5 Ϯ 9.9 W vs. 33.5 Ϯ 9.4 W; P Յ 0.01) in the IC patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC patients. capillary; morphometry; peripheral arterial disease; skeletal muscle; transmission electron microscopy PERIPHERAL ARTERIAL DISEASE (PAD) is an atherosclerotic disease characterized by stenosis or occlusion of the conduit arteries of the lower limbs (20,36). Risk factors associated with PAD include advancing age, cigarette smoking, diabetes mellitus, arterial hypertension, and disturbances in lipid metabolism (12). While many PAD patients are asymptomatic, the arterial narrowing leads to ischemia and hypoxia of the supplied tissues and in severe cases, this manifests as critical limb ischemia (CLI), usually requiring surgical interventions. Intermittent claudication (IC), which is typically described as a cramp-like muscle pain during exercise, is the most commonly reported symptom of PAD and is usually the first noted symptom of the disease.Patients with IC have limited muscular strength and endurance capacities (35), reduced cardiorespiratory fitness (V O 2 peak ), and demonstrate reduced oxygen uptake kinetics at the onset of exercise (6). They also exhibit a significantly reduced tolerance for physical activities such as walking and cycling (2, 13). This reduction in exercise tolerance leads to functional impairments that are similar to those observed in patients with heart failure (31) and contributes to their impaired mobility, reduced quality of life, and elevated risk for the development of cardiovascular complications (17).Impaired limb blood flow is commonly assumed to be the main casual factor of the reduction in exercise tolerance in PAD but cannot entirely explain it. For example, restoration of blood flow capacity with percutaneous endovascular revascularizat...

show abstract

“…In previous studies (18, 25), we have shown that in populations with lower levels of muscle VEGF compared with young healthy individuals, the increase in muscle interstitial VEGF levels during exercise are lower. Moreover, in these subjects, training increased both muscle VEGF protein levels and the magnitude of increase in interstitial VEGF with exercise (18,25). It is, however, unclear whether these findings reflect a direct relationship.…”

Section: Capillary-to-fiber Ratio and Vegfmentioning

confidence: 81%

“…Moreover, basal muscle interstitial levels of TSP-1 in healthy humans have also been reported to be unaltered after 4 wk of moderate intensity exercise training (23). In peripheral arterial disease patients, basal levels of muscle interstitial TSP-1 protein have been reported to be elevated, most likely reflecting capillary regression (25). This is the first evaluation of how resveratrol affects interstitial TSP-1 in skeletal muscle.…”

Section: Muscle Timp-1 and Tsp-1 Levelsmentioning

confidence: 84%

Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men

Gliemann

Olesen

Biensø

et al. 2014

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

modulates the angiogenic response to exercise training in skeletal muscles of aged men. Am J Physiol Heart Circ Physiol 307: H1111-H1119, 2014. First published August 15, 2014; doi:10.1152/ajpheart.00168.2014In animal studies, the polyphenol resveratrol has been shown to influence several pathways of importance for angiogenesis in skeletal muscle. The aim of the present study was to examine the angiogenic effect of resveratrol supplementation with parallel exercise training in aged men. Forty-three healthy physically inactive aged men (65 Ϯ 1 yr) were divided into 1) a training group that conducted 8 wk of intense exercise training where half of the subjects received a daily intake of either 250 mg trans-resveratrol (n ϭ 14) and the other half received placebo (n ϭ 13) and 2) a nontraining group that received either 250 mg trans-resveratrol (n ϭ 9) or placebo (n ϭ 7). The group that trained with placebo showed a ϳ20% increase in the capillary-to-fiber ratio, an increase in muscle protein expression of VEGF, VEGF receptor-2, and tissue inhibitor of matrix metalloproteinase (TIMP-1) but unaltered thrombospodin-1 levels. Muscle interstitial VEGF and thrombospodin-1 protein levels were unchanged after the training period. The group that trained with resveratrol supplementation did not show an increase in the capillary-to-fiber ratio or an increase in muscle VEGF protein. Muscle TIMP-1 protein levels were lower in the training and resveratrol group than in the training and placebo group. Both training groups showed an increase in forkhead box O1 protein. In nontraining groups, TIMP-1 protein was lower in the resveratrol-treated group than the placebo-treated group after 8 wk. In conclusion, these data show that exercise training has a strong angiogenic effect, whereas resveratrol supplementation may limit basal and training-induced angiogenesis.aging; antioxidant; physical activity; capillary; vascular endothelial growth factor SUFFICIENT O2 SUPPLY to the working skeletal muscle is vital to exercise performance, and the capillary network readily adapts according to demand in young healthy subjects (51). With advancing age, as with inactivity, the capillary supply to skeletal muscle decreases (7) and physical performance is reduced (66), eventually affecting activities of daily living and life quality (44,45). Acute physical activity increases the expression of various angiogenic factors in the skeletal muscle of both young and aged subjects (16,57,58), and, over weeks, regular physical activity leads to an increase in skeletal muscle capillarization (1,5,8).

show abstract

Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease

Cited by 54 publications

References 54 publications

Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease

Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease

Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men

Contact Info

Product

Resources

About