Angiogenic therapy of acute myocardial infarction by intrapericardial injection of basic fibroblast growth factor and heparin sulfate: An experimental study

Uchida, Yasumi; Yanagisawa‐Miwa, Atsuko; Nakamura, Fúmitaka; Yamada, Kazuo; Tomaru, Takanobu; Kimura, Kenjiro; Morita, Toshihiro

doi:10.1016/0002-8703(95)90140-x

Cited by 113 publications

(50 citation statements)

References 20 publications

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“…Uchida, et al have reported that bFGF administered into the pericardial space promotes angiogenesis and reduces infarction size in dogs with acute myocardial infarction. 26) bFGF levels in pericardial fluid were not significantly different between patients with ischemic and nonischemic heart disease in this study. This finding may be due to the timing and grade of myocardial ischemia, since there were no patients with acute myocardial infarction in this study.…”

Section: Discussioncontrasting

confidence: 63%

Concentrations of Hepatocyte Growth Factor, Basic Fibroblast Growth Factor, and Vascular Endothelial Growth Factor in Pericardial Fluid and Plasma

et al. 2004

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SUMMARYSome angiogenic factors, including hepatocyte growth factor (HGF), basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF), have been reported to promote angiogenesis and improve myocardial perfusion in experimental models of ischemic heart disease. These factors are produced in various tissues, including myocardium. We measured the concentrations of HGF, bFGF, and VEGF by enzyme-linked immunosorbent assay in plasma and in pericardial fluid sampled during open heart surgery (12 patients with ischemic heart disease and 17 with nonischemic heart disease). HGF levels were significantly higher in plasma than in pericardial fluid (12.0 ± 1.8 versus 0.26 ± 0.04 ng/mL, P < 0.0001). On the other hand, bFGF levels were significantly higher in pericardial fluid than in plasma (243.5 ± 50.9 versus 49.6 ± 7.8 pg/mL, P = 0.009). VEGF levels were not significantly different between pericardial fluid and plasma (47.2 ± 17.6 versus 24.5 ± 3.6 pg/mL, P = 0.23). Concentrations of angiogenic factors in pericardial fluid and in plasma were not significantly different between patients with ischemic and nonischemic heart disease. These results suggest that the production, secretion, and kinetics of HGF, bFGF, and VEGF are different. These angiogenic factors may have different pathophysiologic roles. (Jpn Heart J 2004; 45: 989-998)

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Section: Discussioncontrasting

confidence: 63%

Concentrations of Hepatocyte Growth Factor, Basic Fibroblast Growth Factor, and Vascular Endothelial Growth Factor in Pericardial Fluid and Plasma

et al. 2004

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“…After confirmation of the coronary anatomy by angiography, the left anterior descending artery was occluded by injecting a self-expandable polymer ball (2 × 2 mm) into the artery. 5,14) After recovery from anesthesia, the animals were cared for according to the university guidelines.…”

Section: Myocardial Infarction Modelmentioning

confidence: 99%

“…In experimental studies of therapeutic angiogenesis and arteriogenesis, 5,[11][12][13][14] we identified circulating mononuclear cells (MNCs) expressing β-actin, ie, β-MNCs, in canine coronary microvessels during myocardial ischemia. We therefore examined through what route(s) they migrate into the existing coronary vessels and participate in arteriogenesis and venogenesis.…”

mentioning

confidence: 99%

Migration of Mononuclear Cells Expressing β-Actin Through the Adventitia into Media and Intima in Coronary Arteriogenesis and Venogenesis in Ischemic Myocardium

Uchida

Maezawa

et al. 2012

Int. Heart J.

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SummaryIt was previously thought that arteriogenesis and venogenesis are induced not only by proliferation of vessel-resident smooth muscle cells (SMCs) and endothelial cells (ECs) but also by migration of their precursors. However, it is not well understood through what route(s) the precursors migrate into the existing vessels.We examined through what route or routes circulating mononuclear cells expressing β-actin (β-MNCs), which we identified in canine coronary vessels, migrate into coronary vessel walls and cause arteriogenesis and venogenesis at 1, 2, 4 and 8 weeks after induction of myocardial infarction.The following changes were observed: (1) The β-MNCs migrated via coronary microvessels to the interstitial space at one week; (2) β-MNCs traversed the adventitia into the media and settled in parallel with pre-existing smooth muscle cells (SMCs) in arterioles and arteries and lost β-actin and acquired α-smooth muscle actin (α-SMA) to become mature SMCs at 2-4 weeks; (3) at the same time, other β-MNCs migrated across the adventitia and media into the intima and settled in parallel with pre-existing endothelial cells (ECs) and lost β-actin, while acquiring CD 31 , to become mature ECs, resulting in arteriogenesis; (4) Similarly, β-MNCs migrated into venular and venous walls and became SMCs or ECs, resulting in venogenesis.β-MNCs in the interstitial space expressed CD 34 but not other major vascular cell markers. β-MNCs, possibly a vascular progenitor, migrate not from the lumen but across the adventitia into the media or intima of coronary vessels and transit to SMCs or ECs, and participate in arteriogenesis and venogenesis in ischemic myocardium. (Int Heart J 2012; 53: 54-63)

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“…14,15) The usefulness of EB for regression of atherosclerosis and prevention of coronary re-stenosis has been demonstrated. 16,17) Therefore, this dye can be used clinically not only to evaluate SMBF but also to achieve pin-point guidance of transendocardial angiogenic and myogenic therapies [20][21][22][23][24][25] and intracardiac surgery such as laser myocardial revascularization. 26) Conclusions: SMBF was evaluated by DSC using EB as an indicator of myocardial blood flow in patients with coronary artery disease.…”

Section: Discussionmentioning

confidence: 99%

Imaging of Subendocardial Myocardial Blood Flow by Dye-Staining Cardioscopy in Patients With Coronary Artery Disease

Uchida

Sakurai

et al. 2010

Int. Heart J.

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SummaryThis study was carried out to image subendocardial myocardial blood flow (SMBF) by dye-staining cardioscopy (DSC) in patients with coronary artery disease.In patients with epicardial coronary artery disease, SMBF plays a direct and critical role in determining the extent and severity of cardiac function and symptoms. If SMBF could be clinically imaged instantaneously, the effects of medical and interventional treatment on it can be directly evaluated. However, there are no clinically available methods for direct and real-time imaging of SMBF.Twenty-three patients [6 with chest pain syndrome (CPS); 3 with vasospastic angina pectoris (VSA); 9 with angina pectoris due to organic coronary stenosis (AP); 5 with old myocardial infarction OMI)] underwent DSC of the left ventricle by selective intracoronary injection of 1 mL of 2.5% Evans blue dye solution (EB). Five patients with acute myocardial infarction (AMI) underwent DSC before and after coronary stent deployment.The endocardial surface was stained diffusely blue with EB indicating normal blood flow in patients with CPS; stained in a patchy fashion indicating patchy blood flow in patients with VSA; and stained in a patchy fashion or not stained indicating patchy or no blood flow in those with AP and OMI. Myocardial staining with EB was observed after coronary stent deployment in all patients with AMI, indicating restoration of the SMBF.It is evident that SMBF could be imaged by DSC. This imaging modality is useful for the evaluation of therapies and accurate guidance of transendocardial therapies of the ischemic myocardium. (Int Heart J 2010; 51: 308-311) Key words: Myocardial blood flow, Coronary artery disease, Dye-staining cardioscopy, Evans blue dye S tructurally, the left ventricular wall of the heart comprises 3 myocardial layers, namely the inner oblique, middle circular, and outer oblique myocardial layers.

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Angiogenic therapy of acute myocardial infarction by intrapericardial injection of basic fibroblast growth factor and heparin sulfate: An experimental study

Cited by 113 publications

References 20 publications

Concentrations of Hepatocyte Growth Factor, Basic Fibroblast Growth Factor, and Vascular Endothelial Growth Factor in Pericardial Fluid and Plasma

Concentrations of Hepatocyte Growth Factor, Basic Fibroblast Growth Factor, and Vascular Endothelial Growth Factor in Pericardial Fluid and Plasma

Migration of Mononuclear Cells Expressing β-Actin Through the Adventitia into Media and Intima in Coronary Arteriogenesis and Venogenesis in Ischemic Myocardium

Imaging of Subendocardial Myocardial Blood Flow by Dye-Staining Cardioscopy in Patients With Coronary Artery Disease

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