Angiographic Evaluation of Carotid Artery Grafting with Prefabricated Small-Diameter, Small-Intestinal Submucosa Grafts in Sheep

Pavčnik, Dušan; Obermiller, J.; Uchida, Barry T.; Alstine, William Van; Edwards, James M.; Landry, Gregory J.; Kaufman, John A.; Keller, Frederick S.; Rösch, Josef

doi:10.1007/s00270-008-9449-7

Cited by 31 publications

(22 citation statements)

References 6 publications

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“…However, long-term implantation of cell-free SIS conduits in the arterial circulation showed aneurysm formation and vascular expansions possibly due to thrombosis and matrix degradation [Pavcnik et al, 2009]. Our experiments showed significant platelet attachment on the surface of cell-free SIS within minutes even after treatment with a clinical dose of heparin and warfarin sodium, suggesting that endothelialization is necessary for the development of implantable vascular grafts.…”

Section: Discussionmentioning

confidence: 61%

A Novel Ovine ex vivo Arteriovenous Shunt Model to Test Vascular Implantability

Peng

Schlaich²,

Row³

et al. 2011

Cells Tissues Organs

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The major objective of successful development of tissue-engineered vascular grafts is long-term in vivo patency. Optimization of matrix, cell source, surface modifications, and physical preconditioning are all elements of attaining a compatible, durable, and functional vascular construct. In vitro model systems are inadequate to test elements of thrombogenicity and vascular dynamic functional properties while in vivo implantation is complicated, labor-intensive, and cost-ineffective. We proposed an ex vivo ovine arteriovenous shunt model in which we can test the patency and physical properties of vascular grafts under physiologic conditions. The pressure, flow rate, and vascular diameter were monitored in real-time in order to evaluate the pulse wave velocity, augmentation index, and dynamic elastic modulus, all indicators of graft stiffness. Carotid arteries, jugular veins, and small intestinal submucosa-based grafts were tested. SIS grafts demonstrated physical properties between those of carotid arteries and jugular veins. Anticoagulation properties of grafts were assessed via scanning electron microscopy imaging, en face immunostaining, and histology. Luminal seeding with endothelial cells greatly decreased the attachment of thrombotic components. This model is also suture free, allowing for multiple samples to be stably processed within one animal. This tunable (pressure, flow, shear) ex vivo shunt model can be used to optimize the implantability and long-term patency of tissue-engineered vascular constructs.

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Section: Discussionmentioning

confidence: 61%

A Novel Ovine ex vivo Arteriovenous Shunt Model to Test Vascular Implantability

Peng

Schlaich²,

Row³

et al. 2011

Cells Tissues Organs

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“…In vascular tissue engineering, this paracrine signaling appears to be an important mechanism [18][19][20][21][22] by which tissueengineered blood vessels (TEBVs) remodel. Recent studies have shown they possess a dynamic environment that is repopulated with host cells, [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] such as by the inward migration of vascular smooth muscle cells (SMCs).…”

Section: Introductionmentioning

confidence: 99%

A Cautionary Tale for Autologous Vascular Tissue Engineering: Impact of Human Demographics on the Ability of Adipose-Derived Mesenchymal Stem Cells to Recruit and Differentiate into Smooth Muscle Cells

KrawiecJeffrey

Weinbaum²,

M.³

et al. 2015

Tissue Engineering Part A

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Autologous tissue-engineered blood vessels (TEBVs) generated using adult stem cells have shown promising results, but many preclinical evaluations do not test the efficacy of stem cells from patient populations likely to need therapy (i.e., elderly and diabetic humans). Two critical functions of these cells will be (i) secreting factors that induce the migration of host cells into the graft and (ii) differentiating into functional vascular cells themselves. The purpose of this study was to analyze whether adipose-derived mesenchymal stem cells (AD-MSCs) sourced from diabetic and elderly patients have a reduced ability to promote human smooth muscle cell (SMC) migration and differentiation potential toward SMCs, two important processes in stem cell-based tissue engineering of vascular grafts. SMC monolayers were disrupted in vitro by a scratch wound and were induced to close the wound by exposure to media conditioned by AD-MSCs from healthy, elderly, and diabetic patients. Media conditioned by AD-MSCs from healthy patients promoted the migration of SMCs and did so in a dose-dependent manner; heating the media to 56°C eliminated the media's potency. AD-MSCs from diabetic and elderly patients had a decreased ability to differentiate into SMCs under angiotensin II stimulation; however, only AD-MSCs from elderly donors were unable to promote SMC migration. Gender and body-mass index of the patients showed no effect on either critical function of AD-MSCs. In conclusion, AD-MSCs from elderly patients may not be suitable for autologous TEBVs due to inadequate promotion of SMC migration and differentiation.

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“…SIS from swine displays very low immunogenicity without provoking immune-mediated inflammatory reactions when transplanted. To date, SIS has been extensively used as scaffolds for tissue engineering applications of artery [7], bladder [8], intestinal tract [9,10], and tendon [11]. The US FDA has approved its use in human for urogenital procedures, such as hernia repair, cystoplasties, ureteral reconstructions, stress incontinence, peyronie's disease, penile chordee, even urethral reconstruction for hypospadias and strictures [12e16].…”

Section: Introductionmentioning

confidence: 99%