Background: The long-term survival of patients on hemodialysis (HD) is dependent on adequate HD which depends on quality and degree of functionality of vascular access. The aim of our study is to detect the role of color Doppler ultrasonography in evaluation of arteriovenous fistula (AVF). Materials and methods: Between January 2017 and October 2017, 60 patients had complicated AVF at Qena General Hospital & Qena University Hospital as primary and tertiary centers respectively with the same age group between 18 and 50 years old, had no diabetes mellitus were evaluated using color duplex-Doppler ultrasound (CDUS) after AVF maturation for detection of complications and comparison between primary and tertiary centers of its incidence. Results: The overall results revealed that 11 patients from 30 patients that had been referred to tertiary centers have complicated AVF (36.7%), and 19 patients had no shunt complication (63.3%). Twenty-two patients from 30 patients that had been referred to primary HD centers had complicated shunts. Thrombosis at tertiary centers represents 13.3%, stenosis 3.3% (1 patient), aneurysmal formation had 6.6% for (2 patients), hematoma 6.6%(2 patients), infection 3.3% (1 patient), venous hypertension 3.3% (1 patient), thrombosis and hematoma at primary centers 16.6% (5 patients for each), aneurysmal formation and infection 13.3% for each, and stenosis 10%. Conclusions: CDUS is a readily-available, non-invasive, risk-free, bedside technique that allows precise anatomic knowledge, qualitative, and quantitative data of the upper limb vascular system which greatly help in preoperative planning of AVF creation, assessment of prime time for puncture (maturation), early detection of complications, and choice of appropriate therapeutic procedure for their treatment.