Angiopoietin 1 and angiopoietin 2 in follicular fluid of women undergoing a long protocol

Nishigaki, Akemi; Okada, Hidetaka; Tsuzuki, Tomoko; Cho, Hisayuu; Yasuda, Katsuhiko; Kanzaki, Hideharu

doi:10.1016/j.fertnstert.2011.09.031

Cited by 31 publications

(31 citation statements)

References 40 publications

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“…2005a, 2005b). Recently, it has been suggested that the change in ANGPT1 and ANGPT2 levels may be associated with follicular growth and angiogenesis during the preovulatory period (Nishigaki et al 2011). We observed a higher expression level of ANGPTL1 in the CC MII groups in our study and considered that the results further testified the relationship between angiogenesis and folliculogenesis.…”

Section: Discussionsupporting

confidence: 76%

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

Huang¹,

Hao²,

Shen³

et al. 2013

Reproduction

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Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The abnormalities of endocrine and intra-ovarian paracrine interactions may change the microenvironment for oocyte development during the folliculogenesis process and reduce the developmental competence of oocytes in PCOS patients who are suffering from anovulatory infertility and pregnancy loss. In this microenvironment, the cross talk between an oocyte and the surrounding cumulus cells (CCs) is critical for achieving oocyte competence. The aim of our study was to investigate the gene expression profiles of CCs obtained from PCOS patients undergoing IVF cycles in terms of oocyte maturation by using human Genome U133 Plus 2.0 microarrays. A total of 59 genes were differentially expressed in two CC groups. Most of these genes were identified to be involved in one or more of the following pathways: receptor interactions, calcium signaling, metabolism and biosynthesis, focal adhesion, melanogenesis, leukocyte transendothelial migration, Wnt signaling, and type 2 diabetes mellitus. According to the different expression levels in the microarrays and their putative functions, six differentially expressed genes (LHCGR, ANGPTL1, TNIK, GRIN2A, SFRP4, and SOCS3) were selected and analyzed by quantitative RT-PCR (qRT-PCR). The qRT-PCR results were consistent with the microarray data. Moreover, the molecular signatures (LHCGR, TNIK, and SOCS3) were associated with developmental potential from embryo to blastocyst stage and were proposed as biomarkers of embryo viability in PCOS patients. Our results may be clinically important as they offer a new potential strategy for competent oocyte/embryo selection in PCOS patients.

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Section: Discussionsupporting

confidence: 76%

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

Huang¹,

Hao²,

Shen³

et al. 2013

Reproduction

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“…The high ANGPT-2:ANGPT-1 ratio in developing follicles in vitro was found in a granulosa cell study, where nonluteinized cells produced more ANGPT-2 than ANGPT-1 during a short-term culture. There is also evidence of similar characteristics in the follicular fluid of "lead" follicles from controlled ovarian stimulation protocols in women [28,29].…”

Section: Angiogenesis and Ovarian Functionmentioning

confidence: 92%

“…They are recruited to the endometrium during the transition of endometrial secretory phase and support the deciduas [24]. The main function of uterine natural killer cells is the secretion of cytokines that assist in successful implantation and placental development [29]. Many of those cytokines direct angiogenesis during early pregnancy and influence spiral arteriole modifications later in pregnancy [29].…”

Section: Angiogenesis In Placentation and Embryo Developmentmentioning

confidence: 99%

“…The main function of uterine natural killer cells is the secretion of cytokines that assist in successful implantation and placental development [29]. Many of those cytokines direct angiogenesis during early pregnancy and influence spiral arteriole modifications later in pregnancy [29]. Such angiogenic and angiomodulatory cytokines are ANGPT-1 and ANGPT-2, placental growth factor, VEGF-C, interleukin (IL)-18, and interferon-g [29].…”

Section: Angiogenesis In Placentation and Embryo Developmentmentioning

confidence: 99%

“…Many of those cytokines direct angiogenesis during early pregnancy and influence spiral arteriole modifications later in pregnancy [29]. Such angiogenic and angiomodulatory cytokines are ANGPT-1 and ANGPT-2, placental growth factor, VEGF-C, interleukin (IL)-18, and interferon-g [29]. T and B lymphocytes (and macrophages) surround the embryo after implantation and secrete cytokines [21], such as tumor necrosis factor-a, a proinflammatory cytokine that affects angiogenesis in a context-dependent manner [21].…”

Section: Angiogenesis In Placentation and Embryo Developmentmentioning

confidence: 99%

See 2 more Smart Citations

Molecular regulation and role of angiogenesis in reproduction

Rizov¹,

Andreeva

Dimova

2017

Taiwanese Journal of Obstetrics and Gynecology

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Angiogenesis is an essential process for proper functioning of the female reproductive system and for successful pregnancy realization. The multitude of factors required for physiological angiogenesis and the complexity of regulation of their temporal-spatial activities contribute to aberrations in human fertilization and pregnancy outcomes. In this study, we reviewed the current knowledge of the temporal expression patterns, functions, and regulatory mechanisms of angiogenic factors during foliculogenesis, early implantation/placentation and embryo development, as well as recurrent spontaneous abortions. Angiogenic factors including vascular endothelial growth factors and angiopoietins have documented roles in the development of primordial follicles into mature antral follicles. They also participate in decidualization, which is accompanied by the creation of an extensive network of vessels in the stromal bed that support the growth of the embryo and the placenta, and maintain early pregnancy. During placentation angiogenic and angiomodulatory cytokines, T and B lymphocytes and macrophages affect angiogenesis in a context-dependent manner. Defects in angiogenesis at the maternal-fetal interface contribute to miscarriage in humans. The establishment of more polymorphisms in the genes involved in angiogenesis/vasculogenesis, and their pathological phenotype and expression could give opportunities for prediction, creating a therapeutic strategy, and treatment of diseases related to female reproductive health and problematic conception.

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Platelet‐derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients

Scotti

Parborell

Irusta

et al. 2014

Molecular Reproduction Devel

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Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growth factor (VEGF) are increased in PCOS patients. In this study, we examined the protein levels of platelet-derived growth factor (PDGF) BB and DD (PDGFBB and PDGFDD), angiopoietin 1 and 2 (ANGPT1 and ANGPT2), and their soluble receptor sTIE2 in FF from PCOS and control patients undergoing assisted reproductive techniques. We also analyzed the effect of FF from PCOS and control patients on tight and adherens junction protein expression in an endothelial cell line. PDGFBB and PDGFDD were significantly lower whereas ANGPT1 concentration was significantly higher in FF from PCOS patients than from control patients. No changes were found in the concentration of ANGPT2 or sTIE2. Expression of claudin-5 was significantly increased in endothelial cells incubated for 24 hr in the presence of FF from PCOS versus from control patients, while vascular-endothelial cadherin, β-catenin, and zonula occludens 1 expression were unchanged. The changes observed in the levels of PDGF isoforms and ANGPT1 may prevent VEGF-induced vascular permeability in the PCOS ovary by regulating endothelial-cell-junction protein levels. Restoring the levels of angiogenic factors may provide new insights into PCOS treatment and the prevention of ovarian hyperstimulation syndrome in affected women.

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Angiopoietin 1 and angiopoietin 2 in follicular fluid of women undergoing a long protocol

Cited by 31 publications

References 40 publications

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

Molecular regulation and role of angiogenesis in reproduction

Platelet‐derived growth factor BB and DD and angiopoietin1 are altered in follicular fluid from polycystic ovary syndrome patients

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