2021
DOI: 10.1080/21655979.2021.1985341
|View full text |Cite
|
Sign up to set email alerts
|

Angiopoietin-2 silence alleviates lipopolysaccharide-induced inflammation, barrier dysfunction and endoplasmic reticulum stress of intestinal epithelial cells by blocking Notch signaling pathway

Abstract: Angiopoietin-2 silence alleviates lipopolysaccharide-induced inflammation, barrier dysfunction and endoplasmic reticulum stress of intestinal epithelial cells by blocking Notch signaling pathway

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
6
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 10 publications
(6 citation statements)
references
References 32 publications
0
6
0
Order By: Relevance
“…Recent studies have also identified some new therapeutic pathways and targets. Silencing angiopoietin-2 to block the Notch signaling pathway reduces lipopolysaccharide-induced inflammation, barrier dysfunction, and endoplasmic reticulum stress in intestinal epithelial cells [ 133 ]. The regulatory role of IL1-β on intestinal epithelial cell tight junctions and potential targeted therapies has also been studied [ 134 ].…”
Section: Treatment Of Necmentioning
confidence: 99%
“…Recent studies have also identified some new therapeutic pathways and targets. Silencing angiopoietin-2 to block the Notch signaling pathway reduces lipopolysaccharide-induced inflammation, barrier dysfunction, and endoplasmic reticulum stress in intestinal epithelial cells [ 133 ]. The regulatory role of IL1-β on intestinal epithelial cell tight junctions and potential targeted therapies has also been studied [ 134 ].…”
Section: Treatment Of Necmentioning
confidence: 99%
“…Cells were placed in the radioimmunoprecipitation assay lysis buffer (Beyotime) containing protease inhibitors (PMSF, Beyotime) then collected and lysed [ 22 ]. The bicinchoninic acid assay kit (Beyotime) was used to determine the concentration of protein, and protein was transferred to the polyvinylidene fluoride membranes (Roche).…”
Section: Methodsmentioning
confidence: 99%
“…L6 myotube survival was evaluated using a Cell Counting Kit-8 (CCK8; Beyotime, Shanghai, China) [ 15 ]. Briefly, L6 myotubes were digested and seeded into 96-well plates at density of 1 × 10 4 cells/100 μL/well in triplicate after treatment or transfection, and incubated in a humidified incubator with 5% CO 2 at 37°C.…”
Section: Cell Survival and Apoptosis Assaymentioning
confidence: 99%
“…Absorbance was measured at 450 nm using a microplate reader (Bio-Rad). In addition, the apoptosis of L6 myotubes was measured using the Annexin V-FITC Apoptosis Detection Kit (BD Bioscience, San Jose, CA, USA) using a BD FACSCanto Flow Cytometer (BD Bioscience) [ 15 ].…”
Section: Cell Survival and Apoptosis Assaymentioning
confidence: 99%