Angiopoietin-like protein 2 is a potential biomarker for gastric cancer

Yoshinaga, Takuma; Shigemitsu, Takamasa; Nishimata, Hiroto; Takei, Takayuki; Yoshida, Masahiro

doi:10.3892/mmr.2014.3040

Cited by 17 publications

(17 citation statements)

References 14 publications

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“…Gao et al[39] found that ANGPTL2 inhibited apoptosis by disturbing the tumor intracellular signaling pathway, which accelerated cell growth and facilitated invasion. Other studies have indicated that ANGPTL2 can enhance the activity of the Rec-1/NF-кB pathway, promoting angiogenesis and facilitating the migration of THP-1 by conjugation with integrin[40], and playing a critical role in tumor proliferation and invasion[41-43]. As a consequence, we hypothesize that ANGPTL2 is likely to reduce apoptosis through conjugation with integrin, which facilitates the expression of certain proteins, and activates the corresponding pathway to accelerate gastric cancer cell invasion and migration.…”

Section: Discussionmentioning

confidence: 99%

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Sheng¹,

Chen²,

Tu³

et al. 2016

WJG

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AIMTo explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer.METHODSWestern blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells.RESULTSCompared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells.CONCLUSIONANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer.

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Section: Discussionmentioning

confidence: 99%

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Sheng¹,

Chen²,

Tu³

et al. 2016

WJG

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“…Those reports raised the possibility that ANGPTL2 may also be a candidate biomarker for other types of cancer. We previously demonstrated that ANGPTL2 is upregulated in a gastric cancer cell line and in gastric cancer patients, demonstrating that this protein is a clinically useful biomarker for gastric cancer (11). However, the diagnostic usefulness of ANGPTL2 in CRC has not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Angiopoietin-like protein 2 as a potential biomarker for colorectal cancer

Yoshinaga¹,

Shigemitsu

Nishimata³

et al. 2015

Molecular and Clinical Oncology

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Abstract. Colorectal cancer (CRC) is the third most common malignancy worldwide. Disease progression leads to its spread to other organs, such as the liver, and is associated with higher mortality rates. Early CRC detection is therefore crucial for maximizing the chances of complete cure. The measurement of serum-based tumor biomarkers has shown great potential for the detection of CRC. In this study, we investigated the feasibility of using angiopoietin-like protein 2 (ANGPTL2) as a candidate biomarker for CRC. We first investigated ANGPTL2 expression in 7 CRC cell lines, among which Colo320, NCC-CoCK-115P, Caco-2 and Colo205 exhibited comparatively high ANGPTL2 expression. The serum levels of ANGPTL2 in CRC patients (3.45±1.30 ng/ml) were higher compared with those in healthy controls (2.74±0.64 ng/ml) (P<0.05). A receiver operating characteristic analysis demonstrated that the diagnostic performance of ANGPTL2 was marginally lower compared with that of the established biomarker C-reactive protein, but higher compared with that of carbohydrate antigen 19-9. These results suggested that the simultaneous measurement of ANGPTL2, along with previously established serum biomarkers, may increase the likelihood of early detection of CRC.

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“…Yoshinaga et al [29] studied Ang-like protein 2 (ANGPTL2), a protein that regulates angiogenesis, and reported that the expression and production of ANGPTL2 were both higher in undifferentiated cells (P<0.001 and P<0.050, respectively). Levels of ANGPTL2 in the serum of patients with GC were higher than those in healthy controls (P<0.010).…”

Section: Ang-like Familymentioning

confidence: 99%

Gastric Cancer and Angiogenesis: Is VEGF a Useful Biomarker to Assess Progression and Remission?

et al. 2017

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Gastric cancer (GC) has high mortality owing to its aggressive nature. Tumor angiogenesis plays an essential role in the growth, invasion, and metastatic spread of GC. The aim of this work was to review the angiogenic biomarkers related to the behavior of GC, documented in the literature. A search of the PubMed database was conducted with the MeSH terms: “Stomach neoplasms/blood [MeSH] or stomach neoplasms/blood supply [MeSH] and angiogenic proteins/blood [Major]”. A total of 30 articles were initially collected, and 4 were subsequently excluded. Among the 26 articles collected, 16 examined the role of vascular endothelial growth factor (VEGF), 4 studied endostatin, 3 investigated angiopoietin (Ang)-2, 2 studied the Ang-like protein 2 (ANGTPL2), and 1 each examined interleukin (IL)-12, IL-8, and hypoxia inducible factor. Regarding VEGF, 6 articles concluded that the protein was related to lymph node metastasis or distant metastases. Five articles concluded that VEGF levels were elevated in the presence of GC and decreased following tumor regression, suggesting that VEGF levels could be a predictor of recurrence. Four articles concluded that high VEGF levels were correlated with poor prognosis and lower survival rates. Ang-2 and ANGTPL2 were elevated in GC and associated with more aggressive disease. Endostatin was associated with intestinal GC. VEGF is the most extensively studied angiogenic factor. It is associated with the presence of neoplastic disease and lymph node metastasis. It appears to be a good biomarker for disease progression and remission, but not for diagnosis. The data regarding other biomarkers are inconclusive.

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Angiopoietin-like protein 2 is a potential biomarker for gastric cancer

Cited by 17 publications

References 14 publications

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Angiopoietin-like protein 2 as a potential biomarker for colorectal cancer

Gastric Cancer and Angiogenesis: Is VEGF a Useful Biomarker to Assess Progression and Remission?

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