Angiopteris cochinchinensis de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition

Abstract

Growing demand for treatment options against acute lung injury (ALI) emphasizes studies on plant extracts harboring anti-inflammatory effects. According to GC-MS analysis, Angiopteris cochinchinensis de Vriese consists of various flavonoids with anti-inflammatory activities. Thus, in this study, the anti-inflammatory effects of an extract of Angiopteris cochinchinensis de Vriese (Ac-EE) were assessed using RAW264.6 murine macrophages and a lipopolysaccharide (LPS)-induced ALI model. Ac-EE reduced the nitric oxide production in murine macrophages increased by LPS induction. Moreover, protective effects of Ac-EE on lung tissue were demonstrated by shrinkage of edema and lung injury. Reduced neutrophil infiltration and formation of hyaline membranes were also detected in lung tissues after H&E staining. Semiquantitative RT-PCR, quantitative real-time PCR, and ELISA showed that Ac-EE inhibits the production of proinflammatory mediators, including iNOS and COX-2, and cytokines, such as TNF-α, IL-1β, and IL-6. An Ac-EE-mediated anti-inflammatory response was derived from inhibiting the NF-κB signaling pathway, which was evaluated by luciferase reporter assay and Western blotting analysis. A cellular thermal shift assay revealed that the prime target of Ac-EE in alleviating inflammation was Src. With its direct binding with Src, Angiopteris cochinchinensis de Vriese significantly mitigates lung injury, showing possibilities of its potential as an effective botanical drug.

Antiallergic Effects of Callerya atropurpurea Extract In Vitro and in an In Vivo Atopic Dermatitis Model

“…GC/MS analysis of Ca-EE was performed with an Agilent 8890 GC instrument (Santa Clara, CA, USA) equipped with an Agilent J&W DB-624 Ultra Insert GC column (60 m in length × 250 μm in diameter × 1.40 μm in thickness); mass spectrometry was conducted with an Agilent 5977B MSD instrument (Santa Clara, CA, USA), equipped with a Series II triple-axis detector with a high-energy dynode and long-life electron multiplier, from the Cooperative Center for Research Facilities of Sungkyunkwan University (Gyeonggi-do, Korea). The spectrum of phytochemicals in the National Institute of Standards and Technology library was used to identify the unknown phytochemicals in Ca-EE, as reported previously [ 62 , 63 ].…”

Ginsenosides from Panax ginseng as Key Modulators of NF-κB Signaling Are Powerful Anti-Inflammatory and Anticancer Agents

“…Pulmonary inflammation accumulates neutrophils and macrophages in the alveolar space, interrupting gas exchange [ 114 ]. The TNF-α-induced necrosis of alveolar epithelial cells also results in pulmonary edema via the release of cellular fluid into the airway [ 115 , 116 ]. Moreover, it has been known that individuals with asthma or COPD exhibit the sustained activation of NF-κB signaling in the lung epithelium [ 117 ].…”

Antioxidant, Anti-Inflammatory, Anti-Menopausal, and Anti-Cancer Effects of Lignans and Their Metabolites

“…For instance, the Janus kinase (JAK)-signal transduction and activator of transcription (STAT) signaling pathway is related to T-cell differentiation and B-cell class switching by receiving signals from both type I and type II cytokines [ 66 ]. Meanwhile, the NF-κB and MAPK pathways can be activated by phosphorylation and translocation of NF-κB and AP-1 subunits, respectively [ 67 , 68 ]. Both transcription factors upregulate pro-inflammatory cytokines, including TNF-α, interleukin-1 beta (IL-1β), IL-6, COX-2, and inducible NO synthase (iNOS) [ 69 , 70 , 71 ].…”