Angiotensin-Converting enzyme content of human spermatozoa and its release during capacitation

Foresta, Carlo; Indino, M.; Manoni, Fabio; Scandellari, C.

doi:10.1016/s0015-0282(16)59236-x

Cited by 37 publications

(30 citation statements)

References 11 publications

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“…The ACE activity in semen is enhanced by sexual stimulation (282,313) but is normal in oligospermic men (281). A role of the tACE in capacitation has been suggested by several authors (211,658). Previous reports have suggested that ACE is released during sperm capacitation (356), which was confirmed after exposure to ACE inhibition that affected the acrosome reaction and the ability of human sperm to penetrate an egg (212).…”

Section: Male Reproductive Tractmentioning

confidence: 94%

Physiology of Local Renin-Angiotensin Systems

Paul

Mehr²,

Kreutz³

2006

Physiological Reviews

1,512

1,371

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Since the first identification of renin by Tigerstedt and Bergmann in 1898, the renin-angiotensin system (RAS) has been extensively studied. The current view of the system is characterized by an increased complexity, as evidenced by the discovery of new functional components and pathways of the RAS. In recent years, the pathophysiological implications of the system have been the main focus of attention, and inhibitors of the RAS such as angiotensin-converting enzyme (ACE) inhibitors and angiotensin (ANG) II receptor blockers have become important clinical tools in the treatment of cardiovascular and renal diseases such as hypertension, heart failure, and diabetic nephropathy. Nevertheless, the tissue RAS also plays an important role in mediating diverse physiological functions. These focus not only on the classical actions of ANG on the cardiovascular system, namely, the maintenance of cardiovascular homeostasis, but also on other functions. Recently, the research efforts studying these noncardiovascular effects of the RAS have intensified, and a large body of data are now available to support the existence of numerous organ-based RAS exerting diverse physiological effects. ANG II has direct effects at the cellular level and can influence, for example, cell growth and differentiation, but also may play a role as a mediator of apoptosis. These universal paracrine and autocrine actions may be important in many organ systems and can mediate important physiological stimuli. Transgenic overexpression and knock-out strategies of RAS genes in animals have also shown a central functional role of the RAS in prenatal development. Taken together, these findings may become increasingly important in the study of organ physiology but also for a fresh look at the implications of these findings for organ pathophysiology.

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Section: Male Reproductive Tractmentioning

confidence: 94%

Physiology of Local Renin-Angiotensin Systems

Paul

Mehr²,

Kreutz³

2006

Physiological Reviews

1,512

1,371

View full text Add to dashboard Cite

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“…Angiotensin II is not the only RAS component ascribed with functionality in the testicular RAS. Recent studies have established that Ang (1-7) activation of the Mas receptor is necessary for spermatogenesis in mice and rats [37] and that testicular ACE is necessary for capacitation and subsequent fertilization [38][39][40][41][42]. Thus, this novel Ang II/Ang III binding site could participate in Ang II-regulated male reproductive functions.…”

Section: Tissue Distributionmentioning

confidence: 99%

Distribution of a novel binding site for angiotensins II and III in mouse tissues

Rabey

Karamyan

Speth

2010

Regulatory Peptides

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A novel binding site for angiotensins II and III that is unmasked by parachloromercuribenzoate has been reported in rat, mouse and human brains. Initial studies of this binding site indicate that it is not expressed in the adrenal, liver or kidney of the rat and mouse. To determine if this binding site occurs in other mouse tissues, 8 tissues were assayed for expression of this binding site by radioligand binding assay and compared with the expression of this binding site in the forebrain. Particulate fractions of homogenates of testis, epididymis, seminal vesicles, heart, spleen, pancreas, lung, skeletal muscle, and forebrain were incubated with (125)I-sarcosine(1), isoleucine(8) angiotensin II in the presence or absence of 0.3mM parachloromercuribenzoate plus 10microM losartan and 10microM PD123319 (to saturate AT(1) and AT(2) receptors). Specific (3microM angiotensin II displaceable) high affinity binding occurred in the testis>forebrain>epididymis>spleen>pancreas>lung when parachloromercuribenzoate was present. Binding could not be reliably observed in heart, skeletal muscle and seminal vesicles. High affinity binding of (125)I-sarcosine(1), isoleucine(8) angiotensin II was observed in the absence of parachloromercuribenzoate in the pancreas on occasion. This suggests that this novel angiotensin binding site may have a functional role in these tissues.

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“…Males generated by gene targeting that lack sACE but retain tACE are normally fertile, demonstrating that sACE in males is not essential for their fertility (Hagaman et al ., 1998). In addition, ACE is released from human spermatozoa during acrosome reaction and capacitation (Foresta et al ., 1987). Sperm release of ACE during capacitation may have a physiological role in regulation of the mechanisms that allow the sperm acrosome reaction and thus their fertilizing ability (Foresta et al ., 1991).…”

Section: Introductionmentioning

confidence: 99%

Assay of testicular angiotensin‐converting enzyme activity in human spermatozoa

Kamata¹,

Hu²,

Shibahara

et al. 2001

Int J of Andrology

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The testicular isozyme of angiotensin-converting enzyme (ACE) is associated with male fertility. Spermatozoa from mice lacking ACE showed defects in transport within the oviducts and in binding to zonae pellucidae although the animals had normal sperm count, morphology and motility. In fact, unexplained infertility is difficult to be predicted by conventional parameters such as sperm count. We measured membrane testicular ACE activity in a sperm suspension in PBS and total testis ACE activity in spermatozoa by solubilization with Triton X-100. Total testis ACE activity and membrane testis ACE activity of the same subject were compared in 12 control subjects. We demonstrated that testicular ACE is stable in spermatozoa and the assay of testicular ACE activity is possible. Total testicular ACE activity was approximately twice the membrane testicular ACE activity in all of the subjects tested. The assay of testicular ACE activity in human spermatozoa could be a new method for the assessment of sperm function.

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Angiotensin-Converting enzyme content of human spermatozoa and its release during capacitation

Cited by 37 publications

References 11 publications

Physiology of Local Renin-Angiotensin Systems

Physiology of Local Renin-Angiotensin Systems

Distribution of a novel binding site for angiotensins II and III in mouse tissues

Assay of testicular angiotensin‐converting enzyme activity in human spermatozoa

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