Angiotensin Converting Enzyme Inhibitors, Captopril and Enalaprilat, Augment Bradykinin-Induced Prostacyclin Synthesis in Cultured Rat Vascular Smooth Muscle Cells

Simultaneous Measurements of Cytosolic Free Calcium Level and Prostaglandin Synthesis Reveal a Correlation between Them in Perfused Monolayer of Cultured Rat Vascular Smooth Muscle Cells: Effects of Bradykinin and Angiotensin II.

“…The lower limit of measured 6-keto-PGFIa in the perfusion solution was 3.25 pg/ml. The perfusion solution contained captopril (1,uM) to prevent the kininase activity in VSM cells (Takeuchi et al 1989d) and to detect fully BK-induced 6-keto-PGFIa production. Dose-response relationship study Dose-response relationship between BK and [Ca2+] i or that between BK and 6-ketoPGFIa was examined.…”