Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity, Antioxidant Properties, Phenolic Content and Amino Acid Profiles of Fucus spiralis L. Protein Hydrolysate Fractions

Abstract

Food protein-derived hydrolysates with multi-bioactivities such as antihypertensive and antioxidant properties have recently received special attention since both activities can play significant roles in preventing cardiovascular diseases. This study reports, for the first time, the angiotensin I-converting enzyme (ACE)-inhibition and antioxidant properties of ultrafiltrate fractions (UF) with different molecular weight ranges (<1, 1–3 and ≥3 kDa) obtained from Fucus spiralis protein hydrolysate (FSPH) digested with cellulase–bromelain. The amino acids profile, recovery yield, protein, peptide and total phenolic contents of these FSPH-UF, and the in vitro digestibility of F. spiralis crude protein were also investigated. FSPH-UF ≥3 kDa presented remarkably higher ACE-inhibition, yield, peptide and polyphenolic (phlorotannins) contents. Antioxidant analysis showed that FSPH-UF <1 kDa and ≥3 kDa exhibited significantly higher scavenging of 2,2-diphenyl-1-picrylhydrazyl radical and ferrous ion-chelating (FIC) activity. FSPH-UF ≥3 kDa had also notably higher ferric reducing antioxidant power (FRAP). Strong correlations were observed between ACE-inhibition and antioxidant activities (FIC and FRAP). The results suggest that ACE-inhibition and antioxidant properties of FSPH-UF may be due to the bioactive peptides and polyphenols released during the enzymatic hydrolysis. In conclusion, this study shows the potential use of defined size FSPH-UF for the prevention/treatment of hypertension and/or oxidative stress-related diseases.

Antidiabetic Potential of Marine Brown Algae—a Mini Review

“…Three ultrafiltrate fractions of F. spiralis were obtained after obtaining the protein hydrolysate with the enzymatic digestion by the mixture of two enymes cellulase and bromelain. The results revealed the potent ACE inhibitory activity in fraction 3 (IC 50 : 0.5 mg/ml) than the fraction 1 (IC 50 : 1.85 mg/ml) and fraction 2 (IC 50 : 2 mg/ml) compared to the positive control captopril (IC 50 : 0.163 ng/ml) mainly due to the amino acid composition and phlorotannins released 7 Journal of Diabetes Research during the enzymatic digestion [45]. Cha et al [46] reported the ACE inhibitory activity of some Korean brown seaweeds Sargassum fusiforme, Ishige sinicola, Ecklonia cava, and Sargassum horneri.…”

“…Paiva et al [45] investigated the inhibitory effect of enzymatic protein hydrolysate of brown seaweed Fucus spiralis on ACE. Three ultrafiltrate fractions of F. spiralis were obtained after obtaining the protein hydrolysate with the enzymatic digestion by the mixture of two enymes cellulase and bromelain.…”

Phenolic compounds and bioactivity evaluation of aqueous and methanol extracts ofAllium mongolicumRegel

“…Pancreatic lipase is a key enzyme in the process of dietary fat absorption, which is responsible for 50%-70% of the hydrolysis of dietary triglycerides into monoacylglycerides and free fatty acids, finally been absorbed by enterocytes (Sergent, Vanderstraeten, Winand, Beguin, & Schneider, 2012). ACE belongs to the class of zinc metal proteases that catalyzes the conversion of angiotensin I to a potent vasoconstrictor angiotensin II and also promotes the degradation of the vasodilator bradykinin (Paiva, Lima, Neto, & Baptista, 2017). ACE, another enzyme that have an important impact on metabolic syndrome, plays a pivotal role in the regulation of blood pressure (Villiger, Sala, Suter, & Butterweck, 2015).…”

“…ACE, another enzyme that have an important impact on metabolic syndrome, plays a pivotal role in the regulation of blood pressure (Villiger, Sala, Suter, & Butterweck, 2015). ACE belongs to the class of zinc metal proteases that catalyzes the conversion of angiotensin I to a potent vasoconstrictor angiotensin II and also promotes the degradation of the vasodilator bradykinin (Paiva, Lima, Neto, & Baptista, 2017). Plenty of synthetic drugs, such as captopril, that used in the treatment of hypertension were ACE inhibitors.…”

Effect of Captopril on Brain Oxidative Damage in Pentylenetetrazole-Induced Seizures in Mice<br />

“…Protective Effects of Captopril in Seizure with ACE inhibitors can diminish the oxidative stress. 25,26 Captopril has been shown to act as a potent free radical scavenger. 27 It has been reported that captopril reduces oxidative stress through attenuating of lipid peroxidation in diabetic patients.…”