Angiotensin-I converting enzyme inhibitory and antioxidant activity of bioactive peptides produced by enzymatic hydrolysis of skin from grass carp (Ctenopharyngodon idella)

Abstract

Grass carp skin pieces were homogenized in water and hydrolyzed by Alcalase®, collagenase, proteinase K, and/or trypsin at their optimum conditions. Samples were taken at various degrees of hydrolysis and were evaluated for antioxidant, antimicrobial, and angiotensin-converting enzyme inhibitory activities. Alcalase and collagenase completely hydrolyzed the skin with different rates, and released peptides with antioxidant and angiotensin-converting enzyme-inhibitory activity. These activities increased linearly with increasing degrees of hydrolysis. Subsequent incubation of the collagenase hydrolysates with trypsin slightly increased the antioxidant activity. Proteinase K, although only partially hydrolyzing the skin, also catalyzed the release of peptides with antioxidant and angiotensin-converting enzyme-inhibitory activities. These results show that skin byproducts from grass carp can be a source of bioactive peptides produced by a one-step reaction. Such hydrolysates may be applied in food products to prolong shelf life and provide beneficial effects on blood pressure.

Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells

“…The profile registered by RP-HPLC analysis presented a split peak at 1.174 and 1.343 min ( Figure 1 b). The peaks corresponding to retention times under 5 min were likely to contain small, hydrophilic fragments or free amino acids, as reported for other enzymatic fish hydrolysates [ 36 ].…”

Use of Alcalase in the production of bioactive peptides: A review

“…Collagenase, Proteinase K, Alcalase, and/or trypsin at their optimum conditions were used for the hydrolysis of grass carp (Ctenopharyngodon idella) skin pieces [493].…”

“…Alcalase and collagenase released peptides with angiotensin I-converting enzyme inhibitory activity [493]. In another work, angiotensin I-converting enzyme inhibitory and anticoagulant peptides from tuna cooking juice were prepared by enzymatic hydrolysis with Flavourzyme, pancreatin, Alcalase and pepsin [494].…”

Estudo Retrospectivo Para Pesquisa Do Vírus Zika E Alfavírus Em Amostras De Primatas Não Humanos, Em 2015, No Brasil

“…Esses dados corroboram a pesquisa de Yi et al (2017) no qual a partir da hidrólise enzimática da pele de carpa-do-limo (Ctenopharyngodon idella) encontraram uma capacidade que quelação de ferro de 40% e 60% para os hidrolisados obtidos pela ação associada de colagenase e tripsina por 20,4 horas e 21,9 horas, respectivamente, e de 95% para os hidrolisados gerados pela alcalase® por 1,6 horas. De forma menos expressiva quanto a quelação de ferro, WU et al (2017) através da hidrólise enzimática da gelatina de bacalhau do Pacífico, identificaram uma capacidade de quelação deste íon de 12%, 6% e 9% para os hidrolisados obtidos da proteólise da tripsina, alcalase e flavoenzima, respectivamente, por 120 minutos em condições ótimas para cada enzima.…”