“…The paraffin-embedded tissue sections (4μm thick) were dewaxed in xylene, and rehydrated through graded alcohols as previously reported. 9 Briefly, the sections were incubated overnight with primary antibodies including a mouse monoclonal antibody against macrophages (1:200, Millipore, CA, USA) and a monoclonal antibody against α-smooth muscle actin (SMA, 1:800, Sigma Chemical Co., MO, USA), respectively. The slides were incubated with a biotinylated horse anti-rabbit IgG or an anti-mouse IgG (Vector Laboratories), stained using the ABC-peroxidase kit or ABC-AP (Alkaline phosphatase, Vector Laboratories, CA, USA) and visualized with 3,3'-diaminobenzidine tetrahydrochloride or alkaline phosphatase substrate kit (Sigma, MO, USA).…”