2002
DOI: 10.1016/s1388-9842(02)00005-3
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Angiotensin II subtype 1 (AT1) receptors contribute to ischemic contracture and regulate chemomechanical energy transduction in isolated transgenic rat (αMHC‐hAT1)594–17 hearts

Abstract: Background: The role of AT1 receptors in myocardial ischemia/reperfusion injury is unclear. We, therefore, investigated the effects of the AT1 receptor antagonist irbesartan (Irb) in isolated hearts of selective myocardial AT1 overexpressing transgenic [transgenic(αMHC‐hAT1)594–17] and Sprague–Dawley rats (SD) subjected to ischemia/reperfusion injury. Methods and results: Hearts of 4‐week‐old male SD or transgenic rats were isolated and perfused with Krebs–Henseleit buffer with or without 10 μM Irb in Langendo… Show more

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Cited by 3 publications
(2 citation statements)
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References 33 publications
(49 reference statements)
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“…The animals were anesthetized by an intraperitoneal injection of sodium pentobarbital (50 mg/kg), and heparin (200 U) was administered through the femoral vein to avoid clotting of blood. Then the hearts were rapidly excised, freed of adhering tissue and mounted intact on the Langendorff heart apparatus and perfused retrogradely with Krebs-Henseleit solution [13] . The Krebs-Henseleit solution consisted of the following (mmol/l): NaCl, 118.0; KCl, 4.7; MgCl 2 ؒ 6H 2 O, 0.5; NaHCO 3 , 25.0; NaH 2 PO 4 , 1.0; glucose, 10.0, and CaCl 2 ؒ 2H 2 O, 2.2.…”
Section: Isolated Heart Preparationsmentioning
confidence: 99%
“…The animals were anesthetized by an intraperitoneal injection of sodium pentobarbital (50 mg/kg), and heparin (200 U) was administered through the femoral vein to avoid clotting of blood. Then the hearts were rapidly excised, freed of adhering tissue and mounted intact on the Langendorff heart apparatus and perfused retrogradely with Krebs-Henseleit solution [13] . The Krebs-Henseleit solution consisted of the following (mmol/l): NaCl, 118.0; KCl, 4.7; MgCl 2 ؒ 6H 2 O, 0.5; NaHCO 3 , 25.0; NaH 2 PO 4 , 1.0; glucose, 10.0, and CaCl 2 ؒ 2H 2 O, 2.2.…”
Section: Isolated Heart Preparationsmentioning
confidence: 99%
“…Germline transmission transgenic technology has been used to overexpress the AT 1 R in an attempt to address structure-function aspects of the receptor with limited success. [15][16][17][18][19] Recently, a new technology involving the use of protein transduction domains (PTDs) has been developed as an alternative for an efficient delivery of protein in vivo. 20 It is based on the observation that an 11-amino-acid sequence from the HIV-Tat protein possesses the ability to transport any protein linked to it across the plasma membrane.…”
mentioning
confidence: 99%