AngiotensinII and NGF differentially influence microtubule proteins in PC12W cells: role of the AT2 receptor

Stroth, Ursula; Meffert, Simone; Gallinat, Stefan; Unger, Thomas

doi:10.1016/s0169-328x(97)00298-2

Cited by 85 publications

(63 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in NG108-15 cells, treatment of PC12W cells with Ang II promoted neuronal differentiation (characterized by an increase in neurite elongation) (Meffert et al 1996). In this model, Ang II-induced neurite outgrowth is also associated with enhanced levels of polymerized -tubulin and MAP2 associated with microtubules (Stroth et al 1998), but showed reduced expression of MAP1B (Stroth et al 1998) andneurofilament M (Gallinat et al 1997), two proteins specifically associated with elongation of axons (Gordon-Weeks 1991, Shea & Flanagan 2001. Angiotensin II-induced neurite outgrowth was also studied in rat neuronal cells in culture.…”

Section: At 2 Receptor and Neurite Outgrowthmentioning

confidence: 62%

The angiotensin type 2 receptor of angiotensin II and neuronal differentiation: from observations to mechanisms

Gendron¹,

Payet²,

Gallo‐Payet³

2003

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

The angiotensin II (Ang II) type 2 receptor (AT 2 ) is a member of the seven-transmembrane domain, G-protein coupled receptor family. This receptor is ubiquitously distributed in the fetus but, in most tissues, its expression dramatically falls in the first few hours after birth. Based on this observation, the hypothesis that this receptor could be involved in fetal development was raised and, over the past ten years, many studies have tried to identify a role for the AT 2 receptor using many different tissues and cell lines. To date, one of the major roles associated with the Ang II AT 2 receptor concerns its ability to induce neuronal differentiation. Indeed, in cells of neuronal origin, activation of the AT 2 receptor was shown to induce neurite outgrowth and elongation, modulate neuronal excitability, promote cellular migration and, in particular conditions, induce neuronal cell death. Regarding its signaling mechanisms, the AT 2 receptor still represents one of the most controversial G-protein coupled receptors since it does not stimulate the production of any of the classical second messengers. This review summarizes knowledge of the functions and the signaling mechanisms involved in the actions of the AT 2 receptor in neurons and cells of neuronal origin. Based on its altered expression in neurological disorders, a role for the AT 2 receptor in control of neuronal plasticity is proposed.

show abstract

Section: At 2 Receptor and Neurite Outgrowthmentioning

confidence: 62%

The angiotensin type 2 receptor of angiotensin II and neuronal differentiation: from observations to mechanisms

Gendron¹,

Payet²,

Gallo‐Payet³

2003

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…A highly reproducible signal from the AT 2 receptor seems to be coupling to ion-channel proteins (Fig. 7), such as the hypothalamic neuronal delayed rectifier potassium channel (Kang et al, 1993(Kang et al, , 1994, the T-type calcium channel (Buisson et al, 1992(Buisson et al, , 1995 in other cells, which can suppress or induce cellular growth and differentiation Stoll et al, 1995;Laflamme et al, 1996;Meffert et al, 1996;Munzenmaier and Greene, 1996;Tsuzuki et al, 1996a,b;Gallinat et al, 1998;Stroth et al, 1998;Cote et al, 1999;Gendron et al, 1999) as well as support apoptosis (Yamada et al, 1996;Chamoux et al, 1999;Gallinat et al, 1999).…”

Section: E Signalingmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. XCIX. Angiotensin Receptors: Interpreters of Pathophysiological Angiotensinergic Stimuli

et al. 2015

View full text Add to dashboard Cite

“…However, Ang II prevented the fetal calf serum-as well as epidermal growth factor-induced activation of ERK1 and ERK2 (Bedecs et al 1997). Furthermore, Ang II-induced neurite outgrowth is associated with changes in the levels of microtubuleassociated proteins (MAPs), such as up-regulation of MAP2c in NG108-15 cells and MAP2 in PC12 W cells, and downregulation of MAP1B in PC12 W cells (Laflamme et al 1996;Stroth et al 1998). These studies indicate a specific role for the AT 2 receptor in neuronal cell differentiation and nerve regeneration via regulation of the microtubule network.…”

Section: Discussionmentioning

confidence: 99%

Contribution of bradykinin and nitric oxide to AT₂ receptor‐mediated differentiation in PC12 W cells

Zhao

Biermann

Luther

et al. 2003

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

We investigated the effect of angiotensin II on intracellular cyclic GMP content and neurite outgrowth as an indicator of cell differentiation in PC12 W cells. Neurite outgrowth was examined by phase-contrast microscopy. Outgrown neurites were classified as small, medium and large, and were expressed as neurites per 100 cells. Angiotensin II (10 )7 M) increased the outgrowth of medium and large neurites by mean ± SEM 20.2 ± 2.3 and 6.6 ± 1.4 compared with 1.66 ± 0.5 and 0.1 ± 0.06 neurites per 100 cells in control. Cellular cyclic GMP content increased by 50-250% with angiotensin II at concentrations of 10 )6 -10 )4 M. Both blockade of AT 2 receptors and of nitric oxide synthase markedly reduced angiotensin II-induced neurite outgrowth and cyclic GMP production. In contrast, B 2 receptor blockade had no effect or even increased these angiotensin II effects. Sodium nitroprusside and 8-bromo-cyclic GMP both mimicked the effects of angiotensin II on cell differentiation. The protein kinase G inhibitor KT-5823 inhibited the neurite outgrowth induced by both angiotensin II and 8-bromo-cyclic GMP. Our results demonstrate that angiotensin II can stimulate cell differentiation in PC12 W cells by nitric oxide-related and cyclic GMP-dependent mechanisms. The effects of angiotensin II on cell differentiation and cyclic GMP production were mediated via the AT 2 receptor and further enhanced by bradykinin B 2 receptor blockade. Keywords: angiotensin II, AT 2 receptor, cell differentiation, bradykinin, cyclic GMP, nitric oxide. Two major angiotensin II (Ang II) receptor subtypes, AT 1 and AT 2 , have been identified using specific receptor antagonists such as losartan and PD 123319. Most of the established physiological actions of Ang II, such as regulation of blood pressure and electrolyte homoeostasis, as well as its growth promoting actions, are mediated by the AT 1 receptor. However, results of recent studies in various tissues and cell lines have highlighted a number of actions of Ang II that are mediated by activation of AT 2 receptors, including growth inhibition, differentiation, apoptosis and nerve regeneration (Nakajima et al. 1995;Stoll et al. 1995;Yamada et al. 1996;Lucius et al. 1998).Two major strategies have been developed to block the renin-angiotensin system, first, the blockade of angiotensinconverting enzyme (ACE) by the action of ACE inhibitors and, second, the selective blockade of the AT 1 receptor by AT 1 receptor antagonists. ACE inhibitors inhibit the generation of Ang II and block the effects of the peptide on both the AT 1 and the AT 2 receptor. In addition, ACE inhibition in part prevents the degradation of bradykinin, leading to a stimulation of bradykinin B 2 receptor-mediated release of paracrine mediators, such as nitric oxide (NO) and/or prostaglandins. Bradykinin-mediated actions appear to be of major importance for the organ protective effects of ACE inhibitors . On the other hand, AT 1 receptor antagonists selectively block the AT 1 receptor, leave the AT 2 receptor unopposed and do...

show abstract

AngiotensinII and NGF differentially influence microtubule proteins in PC12W cells: role of the AT2 receptor

Cited by 85 publications

References 36 publications

The angiotensin type 2 receptor of angiotensin II and neuronal differentiation: from observations to mechanisms

The angiotensin type 2 receptor of angiotensin II and neuronal differentiation: from observations to mechanisms

International Union of Basic and Clinical Pharmacology. XCIX. Angiotensin Receptors: Interpreters of Pathophysiological Angiotensinergic Stimuli

Contribution of bradykinin and nitric oxide to AT₂ receptor‐mediated differentiation in PC12 W cells

Contact Info

Product

Resources

About

AngiotensinII and NGF differentially influence microtubule proteins in PC12W cells: role of the AT2 receptor

Cited by 85 publications

References 36 publications

The angiotensin type 2 receptor of angiotensin II and neuronal differentiation: from observations to mechanisms

The angiotensin type 2 receptor of angiotensin II and neuronal differentiation: from observations to mechanisms

International Union of Basic and Clinical Pharmacology. XCIX. Angiotensin Receptors: Interpreters of Pathophysiological Angiotensinergic Stimuli

Contribution of bradykinin and nitric oxide to AT2 receptor‐mediated differentiation in PC12 W cells

Contact Info

Product

Resources

About

Contribution of bradykinin and nitric oxide to AT₂ receptor‐mediated differentiation in PC12 W cells