Angle-dependent strength of a single chemical bond by stereographic force spectroscopy

Abstract

Stereographic force spectroscopy reveals that a chemical bond ruptures along a fixed pathway such that the apparent bond strength strongly depends on the angle of force application.

Single-Chain Inherent Elasticity of Macromolecules: From Concept to Applications

“…In the middle of the 20th century, Flory and other pioneers put forward several statistical mechanical models to semiquantitatively describe the single-chain elasticities of polymers in theory. , With the advent of single-molecule atomic force microscopy (AFM), the elastic behaviors of individual macromolecules have been extensively researched experimentally since the 1990s. − Although great progress has been made toward understanding the single-chain properties of macromolecules, many challenges still remain in this field. − With a backbone and side chains, a polymer chain is more complex than a small molecule. Moreover, a single polymer chain is usually placed in a complicated environment such as solvent, cosolute, and solid surface, which significantly affects the behaviors of the chain.…”

Effects of hydrogen bonds on the single-chain mechanics of chitin

“…14,16 Pyranose ring-based polysaccharides are deeply involved in many vital biological activities such as structural support, energy storage, and cell response. 3,17,18 The use of atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] assists in understanding the structural and functional properties of polysaccharides at the single-molecule level. For a-linked polysaccharides, the chair conformation of sugar rings changes to a boat conformation when the single chain is extended, which will result in a fingerprint plateau in the single-chain force-extension (F-E) curve.…”

Anisotropic Friction in a Ligand-Protein Complex

“…43 As a simple test system, we focus on the well-established biotinstreptavidin (SA) complex. 26,42,44−46 Defining the pulling direction in spherical coordinates, we perform stereographic pulling experiments 29 at different polar angles θ, with unknown azimuthal angle φ, i.e., orientation of the protein. Complementarily, we carry out SMD simulations using a harmonic restraint with well-defined angles θ and φ.…”