Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Abstract

Surface plasmon resonance (SPR) is a real-time, label-free, and high-sensitive detection technology. SPR has been widely used in many applications such as biomolecular interaction analysis, environmental monitoring, and medical diagnostics. However, conventional SPR sensor systems usually require expensive equipment and complicated optics. In this paper, we have demonstrated a rapid prototyping of angle-scanning SPR for bioanalytical investigation. Rapid prototyping was attained by utilizing the FDM (fused deposition modeling) based 3D (three-dimensional) printing technology. Two rotating platforms were employed to drive the laser source and photodiode, respectively. A temperature regulation unit was incorporated to maintain the system temperature in order to reduce the temperature effect. The proposed SPR rapid prototyping yielded a refractive index resolution of 6.4×10 −6 RIU (refractive index unit), and the biotin-avidin system validated the kinetics parameters measurement capability. The obtained results indicated that the FDM 3D printing has great potential for developing rapid-prototyping SPR system.

3-D Printed Instrumentation for Point-of-Use Leaky Waveguide Biochemical Sensor

“…Thus, the RI resolution of the two instruments was broadly comparable. The RI resolution of the LW obtained using the 3D printed instrument was comparable to the values reported in literature for 3D printed SPR instrument [30][31][32][33][34][35][36] (2.37×10 -6 RIU versus 1.7×10 −6 RIU). Based on the RI resolution of the LW obtained using the 3D printed instrument and typical (dn/dc) of 1.89×10 -4 L g −1 for proteins [40], we estimate the LOD for IgG (M w : 150,000 g) to be ~83 nM in the absence of antibodies to enhance the local concentration of protein in the waveguide.…”

“…As a result, 3D printing has increasingly been used to develop a wide range of biochemical analytical systems [27][28][29]. For example, 3D printing has been used to develop point-of-use instrumentation for SPR with a resolution of 6.4×10 −6 to 10 −4 RIU −1 [30,31]. The low cost of SLA and FFF 3D printers means that instruments can be produced in low resource settings without requiring dedicated production lines, and that designs can be modified quickly as requirements change.…”

Gaussian Beam Shaping and Multivariate Analysis in Plasmonic Sensing

“…Sensor sensitivity can be expressed in different units depending on the measured signal or parameter (angle, wavelength, intensity, phase shift, or other SPR-related parameters); moreover, different definitions for resolution can be found even when the sensor resolution is always measured with the same unit, which is, in this case, RIU. For example, according to the definition by Chung et al ., our two SPR systems, with and without beam shaping, present a resolution very similar to some homemade SPR systems − or the portable Corgi IIF SPR instrument from Plasmetrix. However, the definition by Chung et al .…”

Peptide-based antifouling aptasensor for cardiac troponin I detection by surface plasmon resonance applied in medium sized Myocardial Infarction

“…T h e angle-scanning surface plasmon resonance biosensor used in this work was self-designed to detect the interactions between biomolecules on the chip surface [38,40]. The equipment applied the Kretschmann con iguration to achieve attenuated total re lection combined with a liquid low analysis system.…”