Anilinonaphthalene sulfonate and other synthetic ions as mitochondrial membrane penetrants: An H<sup>+</sup> pulse technique study

Jasaitis, Audrius; Chu, La Van; Skulachev, Vladimir P.

doi:10.1016/0014-5793(73)80113-9

Cited by 29 publications

(9 citation statements)

References 15 publications

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“…Jasaitis et al (1973) reached the same conclusion on the basis of observations of the rate of titration of intra-mitochondrial buffer in the presence of ANS. Barker, Barrett-Bee, Berden, McCall and Radda (1974), however, have questioned this conclusion, on the basis of measurement of the rate of penetration of ANS into liposomes using a nuclear magnetic resonance technique.…”

Section: Probe Permeabilitysupporting

confidence: 53%

“…Measurements by Jasaitis, La Van Chu and Skulachev (1973), Layton, Symmons and Williams (1974) and Gains and Dawson (1975a) all indicate that ANS can penetrate the mitochondrial membrane, thus suggesting that the correct explanation of the binding changes must lie in one of the former two categories. Of the hypotheses so far put forward, the three that remain tenable if the probes are permeant ions are those of Skulachev (1970), involving a membrane potential-linked accumulation or extrusion of the probes, Radda and his co-workers (Barrett-Bee & Radda, 1972;Barrett-Bee, Radda & Thomas, 1972;Radda & Vanderkooi, 1972), involving energy-induced conformational changes in the mitochondrial membrane, and Gains and Dawson (1975a) involving a redistribution of bound probe between different sets of binding sites.…”

mentioning

confidence: 95%

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An analysis of the binding of fluorescence probes in mitochondrial systems

Williams¹,

Layton²,

Johnston³

1977

J. Membrain Biol.

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Measurements of the binding of the fluorescent probes 8-anilinonaphthalene-1-sulfonate (ANS) and ethidium ions to whole and disruped mitochondria and submitochondrial particles suggest that the inner mitochondrial membrane is freely permeable to the two probes. Equations relating the binding of permeant probes to the electro-chemical balance across the membrane of vesicular systems are derived and these equations used to analyze Scatchard plots of the binding of the two probes to energized and nonenergized mitochondria and EDTA particles.

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Section: Probe Permeabilitysupporting

confidence: 53%

mentioning

confidence: 95%

An analysis of the binding of fluorescence probes in mitochondrial systems

Williams¹,

Layton²,

Johnston³

1977

J. Membrain Biol.

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“…The work of Jasaitis, La Van Chu and Skulachev (1973), Layton, Symmons and Williams (1974), and Gains and Dawson (1975) indicates that it is. No direct evidence has been published which shows that it is not.…”

Section: Discussionmentioning

confidence: 94%

Transmembrane electrophoresis of 8-anilino-1-naphthalenesulfonate through egg lecithin liposome membranes

Gains

Dawson

1975

J. Membrain Biol.

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Valinomycin has been shown to increase the amount of 8-anilino-1-naphthalenesulfonate (ANS) bound to egg lecithin liposomes and also to increase the maximum fluorescence value, as derived from double reciprocal plots. The assay conditions were such that addition of valinomycin would not produce a transmembrane potential. The formation of a valinomycin potassium ANS complex in the micelle membrane is proposed. This could account for the increase in the maximum fluorescence value and, by acting as an ANS transporter, could also account for the increase in ANS bound. Tributylamine was also shown to increase the binding and maximum fluorescence of ANS. In assay conditions where the addition of valinomycin would produce a transmembrane potential negative inside, the tributylamine-induced fluorescence was reversed. The fluorescense decrease is interpreted as transmembrane electrophoresis of ANS in response to a transmembrane potential.

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“…An alternative interpretation of ANS fluorescence changes during energy conservation in mitochondria is offered by Jasaitis, Kuliene & Skulachev (1971), who consider the ANS fluorescence changes a consequence of an electrophoretic movement of the probe in the electrical potential generated across the mitochondrial membrane. The conclusion of Jasaitis, Van Chu & Skulachev (1973) is based on the assumption that ANS can penetrate across the mitochondrial membrane and behaves analogously to synthetic organic cations and anions which they have utilized as probes for transmembrane potentials.…”

Section: (A) Surf Ace Potentialmentioning

confidence: 99%

The application of fluorescent probes in membrane studies

Azzi

1975

Quart. Rev. Biophys.

228

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Fluorescence has been used in biochemical studies for many years but only recently has the information content and the practical applicability of the fluorescence method been fully realized.Following the early studies of Newton (1954) and Weber (11954) and after the initial utilization of fluorescent probes by Chance and coworkers (Azziet al.1969) and Tasakiet al.(1968), in the study of membranes, the use of fluorescence to provide structural information at microscopic or molecular levels in biological membranes has become widespread. widespread. The application of the fluorescence technique to biological systems has progressed parallel to the development of a theoretical basis for fluorescence data interpretation and the synthesis of a large number of fluorescent probes, organic molecules having fluorescence characteristics that are dependent on their environment.

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Anilinonaphthalene sulfonate and other synthetic ions as mitochondrial membrane penetrants: An H⁺ pulse technique study

Cited by 29 publications

References 15 publications

An analysis of the binding of fluorescence probes in mitochondrial systems

An analysis of the binding of fluorescence probes in mitochondrial systems

Transmembrane electrophoresis of 8-anilino-1-naphthalenesulfonate through egg lecithin liposome membranes

The application of fluorescent probes in membrane studies

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Anilinonaphthalene sulfonate and other synthetic ions as mitochondrial membrane penetrants: An H+ pulse technique study

Cited by 29 publications

References 15 publications

An analysis of the binding of fluorescence probes in mitochondrial systems

An analysis of the binding of fluorescence probes in mitochondrial systems

Transmembrane electrophoresis of 8-anilino-1-naphthalenesulfonate through egg lecithin liposome membranes

The application of fluorescent probes in membrane studies

Contact Info

Product

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Anilinonaphthalene sulfonate and other synthetic ions as mitochondrial membrane penetrants: An H⁺ pulse technique study