Animal and human enteric viruses in water and sediment samples from dairy farms

Staggemeier, Rodrigo; Bortoluzzi, Marina; Heck, Tatiana Moraes da Silva; Luz, Roger Bordin da; Fabres, Rafael Bandeira; Soliman, Mayra Cristina; Rigotto, Caroline; Baldasso, Nelson Antônio; Spilki, Fernando Rosado; Almeida, Sabrina Esteves de Matos

doi:10.1016/j.agwat.2015.01.010

Cited by 33 publications

(18 citation statements)

References 51 publications

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“…Similar trends have been observed in viral abundance in marine and estuarine sediment (Table 1), however, the relative difference in water/sediment abundance cannot be assessed due to the small sample size. Nonetheless, Staggemeier et al (2015a,b) directly compared the concentrations of adenoviruses in corresponding water and sediment samples derived from freshwater streams, dams, and springs and found that the viral abundance in sediment was significantly higher than in the overlying water. Importantly, they found that adenoviruses may be present in sediment in the absence of the virus in the water column.…”

Section: Abundance and Distribution Of Fecally Derived Bacteria And Vmentioning

confidence: 99%

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

et al. 2016

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The long term survival of fecal indicator organisms (FIOs) and human pathogenic microorganisms in sediments is important from a water quality, human health and ecological perspective. Typically, both bacteria and viruses strongly associate with particulate matter present in freshwater, estuarine and marine environments. This association tends to be stronger in finer textured sediments and is strongly influenced by the type and quantity of clay minerals and organic matter present. Binding to particle surfaces promotes the persistence of bacteria in the environment by offering physical and chemical protection from biotic and abiotic stresses. How bacterial and viral viability and pathogenicity is influenced by surface attachment requires further study. Typically, long-term association with surfaces including sediments induces bacteria to enter a viable-but-non-culturable (VBNC) state. Inherent methodological challenges of quantifying VBNC bacteria may lead to the frequent under-reporting of their abundance in sediments. The implications of this in a quantitative risk assessment context remain unclear. Similarly, sediments can harbor significant amounts of enteric viruses, however, the factors regulating their persistence remains poorly understood. Quantification of viruses in sediment remains problematic due to our poor ability to recover intact viral particles from sediment surfaces (typically <10%), our inability to distinguish between infective and damaged (non-infective) viral particles, aggregation of viral particles, and inhibition during qPCR. This suggests that the true viral titre in sediments may be being vastly underestimated. In turn, this is limiting our ability to understand the fate and transport of viruses in sediments. Model systems (e.g., human cell culture) are also lacking for some key viruses, preventing our ability to evaluate the infectivity of viruses recovered from sediments (e.g., norovirus). The release of particle-bound bacteria and viruses into the water column during sediment resuspension also represents a risk to water quality. In conclusion, our poor process level understanding of viral/bacterial-sediment interactions combined with methodological challenges is limiting the accurate source apportionment and quantitative microbial risk assessment for pathogenic organisms associated with sediments in aquatic environments.

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Section: Abundance and Distribution Of Fecally Derived Bacteria And Vmentioning

confidence: 99%

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

et al. 2016

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“…No Estado do Rio Grande do Sul, no município de Tenente Portela, a presença de AdV na porção de confinamento do SAG demonstrou contaminação em 100% dos pontos amostrados em fazendas de produção leiteira (Spilki et al, 2013), podendo indicar contaminação proveniente das áreas de recarga do aquífero. O município de Riozinho apresentou contaminação por AdV e RV em 50% dos poços de propriedades rurais e em Rolante, 66,6% e 55,5%, respectivamente (Staggemeier et al, 2015). Em amostras provenientes do município de Taquara (RS), a frequência de contaminação era de 66,6% para RV, 50% para AdV e 16,6% para EV (dados não publicados).…”

Section: Resultsunclassified

Contaminação viral e bacteriana em águas subterrâneas na porção aflorante do Aquífero Guaraní, município de Ivoti, RS

Luz¹,

Staggemeier²,

Fratta³

et al. 2017

Rev. ambiente água

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RESUMOO abastecimento de água do município de Ivoti provém unicamente de origem subterrânea. A potabilidade da água subterrânea do município foi avaliada através da análise de marcadores bacterianos (Escherichia coli e coliformes totais) e virais (adenovírus, enterovírus e rotavírus) de contaminação. Vinte e sete amostras de água provenientes de poços artesianos, poços cavados e fontes foram coletadas entre fevereiro e abril de 2011. A determinação do número mais provável (NMP) de E. coli e coliformes totais em 100 mL foi realizada pelo método dos tubos múltiplos. Os marcadores virais foram analisados em alíquotas de 500 mL por reação em cadeia da polimerase (PCR). Coliformes totais foram encontrados em 66,6% das amostras. A contaminação encontrada para coliformes termotolerantes foi de 25,9%, 40,7% para adenovírus e 22,2% para rotavírus. Nenhuma amostra apresentou enterovírus. A presença de coliformes e de vírus nas amostras indica contaminação fecal da água subterrânea no local de estudo, situado na porção aflorante do Aquífero Guarani e alerta para a necessidade de precaução quanto ao uso destes mananciais para abastecimento público, captação individual e cultivo de hortaliças.Palavras-chave: adenovírus, Escherichia coli, enterovírus, rotavírus. Viral and bacterial contamination in groundwater from the GuaraníAquifer's recharge area, Ivoti municipality, RS ABSTRACTThe water supply of the municipality of Ivoti comes solely from an underground source. The potability of groundwater in the municipality was evaluated by analyzing bacterial (Escherichia coli and total coliforms) and viral (adenovirus, enterovirus and rotavirus) markers for contamination. Twenty-seven water samples from artesian wells, dug wells and springs were collected between February and April 2011. The determination of the most probable number

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“…It was also observed that lettuces washed at the last stage of production do not represent a benefit for virus removal, since the waters used are also contaminated. Enteric viruses including EV have been used as indicators of sanitation and hygiene practices at food production sites on dairy and swine farms (Fongaro et al, 2014;Kokkinos et al, 2012;Lachapelle, Letellier, Fravalo, Brassard, & L'Homme, 2017;Maunula et al, 2013;Staggemeier et al, 2015;Yavarmanesh, Alum, & Abbaszadegan, 2015).…”

Section: Re Sults and Discussionmentioning

confidence: 99%

Dissemination of enteroviruses in the production chain of organic lettuce in Rio de Janeiro, Brazil

et al. 2018

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This study aimed to survey the environmental dissemination of enterovirus ( EV ) in a site of organic lettuce situated in the mountainous region of the state of Rio de Janeiro, Brazil. For this purpose, a total of 96 environmental samples, including water and lettuce samples obtained in different stages of the production chain (e.g., irrigation water, seedlings, lettuces grown, and washed lettuces ready‐to‐eat), were analyzed. EV genomes were detected in 12.5% (12/96) of the tested samples (eight from irrigation water and 4 from lettuce samples). Levels of viral concentration ranged from 3.37 × 10 3 to 4.72 × 10 6 genomic copies per liter (gc L −1 ) and from 2.14 × 10 4 to 5.56 × 10 4 genome copies per 25 grams (gc 25 g −1 ) for the water and lettuce samples, respectively. Such findings suggest that the use of viruses as human fecal contamination markers must be considered in order to improve food safety in organic supply chains.

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Animal and human enteric viruses in water and sediment samples from dairy farms

Cited by 33 publications

References 51 publications

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

Contaminação viral e bacteriana em águas subterrâneas na porção aflorante do Aquífero Guaraní, município de Ivoti, RS

Dissemination of enteroviruses in the production chain of organic lettuce in Rio de Janeiro, Brazil

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