Animal‐cell culture media: History, characteristics, and current issues

Yao, Tatsuma; Asayama, Yuta

doi:10.1002/rmb2.12024

Cited by 394 publications

(335 citation statements)

References 134 publications

(177 reference statements)

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“…For this experiment, we test the OECT device using a DMEM solution that contains fetal bovine serum with a biologically relevant glucose concentration (15 mM). Cell culture medium contains several electroactive components, which can influence the drain current output, at the given potential (Yao & Asayama, ) as seen in the precondition stage of Figure a. The magnitude of the current drastically drops to the μA range when Fc is added.…”

Section: Resultsmentioning

confidence: 99%

A highly sensitive molecular structural probe applied to in situ biosensing of metabolites using PEDOT:PSS

Pappa²,

Pitsalidis³

et al. 2019

Biotech & Bioengineering

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A large amount of research within organic biosensors is dominated by organic electrochemical transistors (OECTs) that use conducting polymers such as poly(3,4‐ethylene dioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS). Despite the recent advances in OECT‐based biosensors, the sensing is solely reliant on the amperometric detection of the bioanalytes. This is typically accompanied by large undesirable parasitic electrical signals from the electroactive components in the electrolyte. Herein, we present the use of in situ resonance Raman spectroscopy to probe subtle molecular structural changes of PEDOT:PSS associated with its doping level. We demonstrate how such doping level changes of PEDOT:PSS can be used, for the first time, on operational OECTs for sensitive and selective metabolite sensing while simultaneously performing amperometric detection of the analyte. We test the sensitivity by molecularly sensing a lowest glucose concentration of 0.02 mM in phosphate‐buffered saline solution. By changing the electrolyte to cell culture media, the selectivity of in situ resonance Raman spectroscopy is emphasized as it remains unaffected by other electroactive components in the electrolyte. The application of this molecular structural probe highlights the importance of developing biosensing probes that benefit from high sensitivity of the material's structural and electrical properties while being complimentary with the electronic methods of detection.

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Section: Resultsmentioning

confidence: 99%

A highly sensitive molecular structural probe applied to in situ biosensing of metabolites using PEDOT:PSS

Pappa²,

Pitsalidis³

et al. 2019

Biotech & Bioengineering

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“…The high concentration of TAFs explains why MED12 mutant cells are quickly lost from the primary culture of LMs (25,26); fibroblasts attach the culture dish better and grow faster than SMCs in standard culture conditions, conditions originally developed to optimize fibroblast growth (27). Overgrowth of fibroblasts is a classic problem for the primary culturing of SMCs including MM, and many techniques to repress the growth of fibroblasts in SMC culture have been proposed (20,28–32).…”

Section: Discussionmentioning

confidence: 99%

Subtype-Specific Tumor-Associated Fibroblasts Contribute to the Pathogenesis of Uterine Leiomyoma

Wu¹,

Serna²,

Thomas³

et al. 2017

Cancer Research

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Recent genomic studies have identified subtypes of uterine leiomyoma (LM) with distinctive genetic alterations. Here we report the elucidation of the biological characteristics of the two most prevalent LM subtypes, MED12 mutant (MED12-LM) and HMGA2-overexpressing (HMGA2-LM) LM. Since each tumor carries only one genetic alteration, both subtypes are considered to be monoclonal. Approximately 90% of cells in HMGA2-LM were smooth muscle cells (SMC) with HMGA2 overexpression. In contrast, MED12-LM consisted of similar numbers of SMC and non-SMC, which were mostly tumor-associated fibroblasts (TAF). Paradoxically, TAF carried no mutations in MED12, suggesting an interaction between SMC and TAF to coordinate their growth. The higher amount of ECM in MED12-LM than HMGA2-LM was partially due to the high concentration of collagen-producing TAF. SMC growth in a xenograft assay was driven by progesterone in both LM subtypes. In contrast, TAF in MED12-LM proliferated in response to estradiol, whereas progesterone had no effect. The high concentration of estrogen-responsive TAF in MED12-LM explains the inconsistent discoveries between in vivo and in vitro studies on the mitogenic effect of estrogen and raises questions regarding the accuracy of previous studies utilizing MED12-LM cell culture. In addition, the differential effects of estradiol and progesterone on these LM subtypes emphasize the importance of subtypes and genotypes in designing non-surgical therapeutic strategies for LM.

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“…The composition of this medium is standardized and therefore wear markers can be easily quantified using a wide range of assays and analysis methods. In addition, cell-culture medium is able to nourish the tissue and keep it alive throughout testing [10]. Despite these positive characteristics, the rheological properties of cell-culture medium make it a poor substitute for the joints natural lubricant, synovial fluid (SF).…”

Section: Introductionmentioning

confidence: 99%

Phospholipid Vesicles in Media for Tribological Studies against Live Cartilage

et al. 2018

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Introduction Pre-clinical testing of hemiarthroplasty devices requires that the tribological conditions present in vivo with live cartilage be closely duplicated. A current limitation in the tribological testing of live cartilage involves the use of cell-culture media as lubricant. Study Aim to develop and test a new hyaluronan-phospholipid based medium (HA–phospholipid medium) that combines the rheological and frictional properties of synovial fluid with the nourishing properties of culture media to keep cells alive. Materials and Methods The HA–phospholipid medium consisted of culture medium with added phospholipid dipalmitoylphosphatidylcholine (0.3 mg/mL), and hyaluronic acid (2.42 mg/mL). A standard cell culture medium was used as the control. The rheology of each medium was determined using a flat plate configuration. Bovine calf cartilage was used to assess cell viability and friction in each medium. For friction measurements, a cobalt-chrome alloy ball was articulated against cartilage disks immersed in medium. Results Lipid vesicles 0.1 to 50 μm in diameter were identified in the HA–phospholipid medium. Cartilage cell viability was significantly higher in the HA–phospholipid medium (62% ± 8%, 95% CI) than in control medium (49.5% ± 5%) (p = 0.009). The HA–phospholipid medium exhibited strong shear-thinning behavior, similar to synovial fluid, with viscosities ~100-fold higher at 10 s−1 and 5-fold higher at 20,000 s−1 than the approximately Newtonian control medium. The HA–phospholipid medium also yielded 20% lower friction values than the control medium after one hour of testing. Conclusions The rheological and friction results indicate that the HA–phospholipid medium is superior to the control cell culture medium in emulating the shear thinning and lubricative properties of natural synovial fluid, making it more clinically relevant for in vitro wear and friction testing with live cartilage.

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Animal‐cell culture media: History, characteristics, and current issues

Cited by 394 publications

References 134 publications

A highly sensitive molecular structural probe applied to in situ biosensing of metabolites using PEDOT:PSS

A highly sensitive molecular structural probe applied to in situ biosensing of metabolites using PEDOT:PSS

Subtype-Specific Tumor-Associated Fibroblasts Contribute to the Pathogenesis of Uterine Leiomyoma

Phospholipid Vesicles in Media for Tribological Studies against Live Cartilage

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