Animal Cells Used in Manufacturing

Bardouille, Claudia

doi:10.1002/0471250589.ebt016

Cited by 2 publications

(2 citation statements)

References 75 publications

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“…Organotypical systems, as, e.g., artificial liver, bone, or skin are constructed using three-dimensional matrices. Encapsulation of cells followed by transplantation into patients could provide treatment or cure for various illnesses (BARDOUILLE, 1999). Since new methods for the derivation of embryonic stem (ES) cells and progenitor cells, their expansion ex vivo and modification have been successfully established, multiple cell types might be available in the future (HEATH, 2000).These could be used for tissue and organ repair as well as tumor modeling investigating the use of vaccines or gene therapy.…”

Section: Trends and Future Developmentsmentioning

confidence: 99%

Maintenance of Cell Cultures‐with Special Emphasis on Eukaryotic Cells

Bardouille¹

2001

Biotechnology

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Section: Trends and Future Developmentsmentioning

confidence: 99%

Maintenance of Cell Cultures‐with Special Emphasis on Eukaryotic Cells

Bardouille¹

2001

Biotechnology

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“…[1][2][3] The production of viral vaccines in mammalian cell lines is nowadays of tremendous magnitude.…”

Section: Introductionmentioning

confidence: 99%

Development of an animal‐component free medium for vero cells culture

Rourou

Ark²,

Velden³

et al. 2009

Biotechnology Progress

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This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that affect cell growth. Using Medium 199 or MEM as a basal medium, a serum-free medium (SFM) referred as IPT-SFM that only enclosed transferrin as a component of animal origin was developed at first. Then, the composition of IPT-SFM was further improved to obtain an animal-component free medium named IPT-AFM. IPT-AFM contains M199 as a basal medium, plant hydrolysates, epidermal growth factor, ethanolamine, ferric citrate, and vitamin C. Among various plant hydrolysates, specific combinations of soy (Hypep 1510) and wheat gluten (Hypeps 4601 and 4605) hydrolysates, were identified to promote cell growth; whereas individual Hypeps had a minor positive effect on cell growth. Nevertheless, the removal of serum did influence cell attachment. Coating tissue-culture flasks with teleostean, a product extracted from cold water fish skin, had not only enhanced cell attachment but also improved cell growth performance in static cultures. Different non-animal proteases were also assessed as an alternative to trypsin. TrypLE Select, a recombinant trypsin, gave the best cell growth performances. Kinetics of cell growth in IPT-AFM were investigated in T-flasks, cell growth was comparable with that obtained in MEM+10% fetal calf serum (FCS). A mean cell division number equal to 2.26 +/- 0.18 and a specific growth rate micro 0.019 +/- 0.003 h(-1) were achieved in IPT-AFM.

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Animal Cells Used in Manufacturing

Cited by 2 publications

References 75 publications

Maintenance of Cell Cultures‐with Special Emphasis on Eukaryotic Cells

Maintenance of Cell Cultures‐with Special Emphasis on Eukaryotic Cells

Development of an animal‐component free medium for vero cells culture

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