Animal models of chronic obstructive pulmonary disease

Dawkins, Paul

doi:10.1136/thorax.56.12.972

Cited by 51 publications

(30 citation statements)

References 42 publications

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“…These models provide rapid, significant and reproducible results. 25 Many previous studies have investigated exercise training and emphysema, but until now, to our knowledge, there have been no studies that aimed to compare the effects of high and moderate exercise training in an animal model of emphysema. The protocol of treadmill high intensity exercise training that we used was similar to the protocols used in rats in previous study in our laboratory.…”

Section: B Discussionmentioning

confidence: 99%

High intensity exercise training worsens alveolar destruction in pulmonary emphysema rats

Barnabé¹,

Lopes²,

Saraiva-Romanholo³

et al. 2014

Medical Express

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OBJECTIVE: Investigation of standard intensities of physical exercise is important to better comprehend and develop rehabilitation programs for emphysema. We aimed to evaluate the effects of different intensities (moderate and high-intensity) of physical exercise on the development of a protease-induced (papain intratracheal instillation) emphysema in rats. METHODS: Male Wistar rats were randomly separated into five groups that received intratracheal instillation of papain solution or vehicle: (i) papain high intensity exercise, (ii) papain moderate exercise, (iii) saline high intensity exercise, (iv) saline sedentary and (v) papain sedentary. Forty days after intratracheal instillation, the exercise groups were submitted to an exercise-training protocol on a treadmill during 10 weeks, 5 days/week, at 0.9 km/h (Papain and saline high exercise), or at 0.6 km/h (papain moderate exercise). We measured respiratory system elastance and resistance, the collagen fiber lung parenchyma, and the pulmonary mean linear intercept. RESULTS: All animal groups that received papain instillation presented higher alveolar wall destruction compared to animals that received only saline solution. The papain high intensity exercise group presented higher values of mean linear intercept compared to emphysema groups that were trained at a moderate intensity or not submitted to exercise. CONCLUSION: High intensity exercise training worsened alveolar destruction in an experimental model of emphysema in rats when compared to moderate intensity exercise, or to no exercise.

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Section: B Discussionmentioning

confidence: 99%

High intensity exercise training worsens alveolar destruction in pulmonary emphysema rats

Barnabé¹,

Lopes²,

Saraiva-Romanholo³

et al. 2014

Medical Express

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“…To functionally characterize genes identified from human lung function GWAS, animal models are a useful tool to better understand the role of a given gene within the whole organism and the lung in situ (Dawkins & Stockley, 2001). The use of mice in research has always been a controversial issue, and a full understanding of both advantages and disadvantages to the study of human health and disease is essential.…”

Section: Mouse Models For Respiratory Research and The Translation Ofmentioning

confidence: 99%

Translating Lung Function Genome-Wide Association Study (GWAS) Findings

Kheirallah

Miller

Hall

et al. 2016

Advances in Genetics

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“…According to Dawkins and Stockley (2001), the rats represent the best option for animal models of illness, because its genoma has been extensively studied and its sequence shows similarities with the human genoma 8 . Meuwissen and Berns (2005) affirm that the spontaneous lung tumors in rats are similar in morphology, hystopathology and molecular characteristics of the human adenocarcinomas.…”

Section: Discussionmentioning

confidence: 99%

“…As such, they have had a major impact on the investigation of many medicals conditions. The mouse provides the best choice for an animal model because the mouse genome has been extensively studied and sequenced and close similarities exist with the human genome 8 . In parallel with studies in humans, these animal models can be used to evaluate methods of possible chemoprevention and early detection 9 .…”

Section: Introductionmentioning

confidence: 99%

Experimental model of pulmonary carcinogenesis in Wistar rats

Silva¹,

Silva²,

Pereira³

et al. 2007

Acta Cir. Bras.

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Purpose:To elaborate an experimental model of pulmonary carcinogenesis in Wistar rats. Methods: Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%, a polycyclic aromatic hydrocarbon widely known by its power of tumoral induction. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 10 mg/kg; e B[a]P Group 20mg/ kg, submitted to euthanasia 08, 10, 12 and 14 weeks after the experimental procedure. The pulmonary sections had been colored by hematoxilin-eosin (HE) and submitted to the morphometrical analysis to describe the tissue alterations. Results: The presence of diffuse inflammatory alterations was observed in all groups, however, at the analysis of the pulmonary tissue of the experimental groups, it had been observed hyperplasic alterations (BALT hyperplasia), and in one of the animals of the experimental group 20mg/kg (12 weeks), it was noticed the presence of cellular epithelial tracheal pleomorphism, suggesting the adenocarcinoma formation in situ. Conclusion: The main secondary alterations to the intra-pulmonary instillation of B[a]P in Wistar rats were: cellular proliferation, inflammatory alterations of several degrees and nodular lymphoid hyperplasias. The association of an activator agent of the pulmonary metabolic reply is necessary to establish the ideal reply-dose to the development of the lung cancer. Key words: Carcinogenesis. Lung Cancer. Benzo[a]pyrene. Neoplasms. Experimental model. RESUMOObjetivo: Elaborar um modelo experimental de carcinogênese pulmonar em ratos wistar. Métodos: Rattus norvegicus albinus, linhagem Wistar foram submetidos a instilação intra-pulmonar da diluição em álcool 70% de Benzo[a]pireno (B[a]P), um hidrocarboneto aromático policíclico amplamente conhecido por seu poder de indução tumoral. Foram formados três grupos experimentais com 08 animais cada: Grupo Controle (álcool 70%); Grupo B[a]P 10 mg/kg; e Grupo B[a]P 20mg/ kg, submetidos a eutanásia 08, 10, 12 e 14 semanas após o procedimento experimental. As secções pulmonares foram coradas por HE e submetidas a análise morfométrica para descrição das alterações teciduais. Resultados: em todos os grupos observou-se a presença de alterações inflamatórias difusas, porém na análise do tecido pulmonar dos grupos experimentais, observou-se alterações hiperplásicas (hiperplasia de BALT), e em um dos animais do grupo experimental 20mg/kg (12 semanas) notou-se a presença de pleomorfismo celular epitelial traqueal, sugerindo a formação de adenocarcinoma in situ. Conclusão: as principais alterações secundárias à instilação intra-pulmonar de B[a]P em ratos Wistar foram: proliferação celular, alterações inflamatórias de diversos graus e hiperplasias nodulares linfóides. A associação de um agente ativador da resposta metabólica pulmonar pode ser necessária para estabelecimento da dose-resposta ideal ao desenvolvimento do câncer de pulmão. Descritores: Carcinogênese. Câncer de...

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Animal models of chronic obstructive pulmonary disease

Cited by 51 publications

References 42 publications

High intensity exercise training worsens alveolar destruction in pulmonary emphysema rats

High intensity exercise training worsens alveolar destruction in pulmonary emphysema rats

Translating Lung Function Genome-Wide Association Study (GWAS) Findings

Experimental model of pulmonary carcinogenesis in Wistar rats

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