Animal Models of Inflammatory Bowel Disease

Mizoguchi, Atsushi

doi:10.1016/b978-0-12-394596-9.00009-3

Cited by 225 publications

(216 citation statements)

References 236 publications

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“…To date more than 60 different colitis models have been developed, which differ in several aspects, such as pathomechanism, clinical/histological picture and cytokine profile (Hoffmann et al 2002;Mizoguchi 2012;Wirtz and Neurath 2007). Consquently, a drug maybe effective in one, but ineffective in other model of intestinal inflammation depending on its mechanism of action (Park et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Analysing the effect of I1 imidazoline receptor ligands on DSS-induced acute colitis in mice

et al. 2016

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Imidazoline receptors (IRs) have been recognized as promising targets in the treatment of numerous diseases, and moxonidine and rilmenidine, agonists of I1-IRs are widely used as antihypertensive agents. Some evidence suggests that IR ligands may induce anti-inflammatory effects acting on I1-IRs or other molecular targets, which could be beneficial in patients with inflammatory bowel disease (IBD). On the other hand, several IR ligands may stimulate also alpha2-adrenoceptors, which was earlier shown to inhibit, but in more recent studies to rather aggravate colitis. Hence, this study aimed to analyse for the first time the effect of various I1-IR ligands on intestinal inflammation. Colitis was induced in C57BL/6 mice by adding dextran sulfate sodium (DSS) to the drinking water for 7 days. Mice were treated daily with different IR ligands; moxonidine and rilmenidine (I1-IR agonists), AGN 192403 (highly selective I1-IR ligand, putative antagonist), efaroxan (I1-IR antagonist), as well as with the endogenous IR agonists agmatine and harmane. It was found that moxonidine and rilmenidine at clinically relevant doses, similarly to the other IR ligands, do not have a significant impact on the macroscopic and histological signs of DSS-evoked inflammation. Likewise, colonic myeloperoxidase and serum interleukin-6 levels remained unchanged in response to these agents. Thus, our study demonstrates that imidazoline ligands do not influence significantly the severity of DSS-colitis in mice and suggest that they probably neither affect the course of IBD in humans. However, the translational value of these findings needs to be verified with other experimental colitis models and human studies.

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Section: Discussionmentioning

confidence: 99%

Analysing the effect of I1 imidazoline receptor ligands on DSS-induced acute colitis in mice

et al. 2016

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“…In mice, there is a wide range of genetic, spontaneous and chemical models [23][24][25] that have been used in an attempt to find answers to different about IBD issues. Beginning some years ago, an increasing variety of zebrafish IBD chemical models have arisen, which are based on models previously tested in mice.…”

Section: Ibd Modelsmentioning

confidence: 99%

Inflammatory diseases modelling in zebrafish

Fénero

Flores

Câmara

2016

WJEM

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The ingest of diets with high content of fats and carbohydrates, low or no physical exercise and a stressful routine are part of the everyday lifestyle of most people in the western world. These conditions are triggers for different diseases with complex interactions between the host genetics, the metabolism, the immune system and the microbiota, including inflammatory bowel diseases (IBD), obesity and diabetes. The incidence of these disorders is growing worldwide; therefore, new strategies for its study are needed. Nowadays, the majority of researches are in use of murine models for understand the genetics, physiopathology and interaction between cells and signaling pathways to find therapeutic solutions to these diseases. The zebrafish, a little tropical water fish, shares 70% of our genes and conserves anatomic and physiological characteristics, as well as metabolical pathways, with mammals, and is rising as a new complementary model for the study of metabolic and inflammatory diseases. Its high fecundity, fast development, transparency, versatility and low cost of maintenance makes the zebrafish an interesting option for new researches. In this review, we offer a discussion of the existing genetic and induced zebrafish models of two important Western diseases that have a strong inflammatory component, the IBD and the obesity. Core tip:The western lifestyle with a high fat and carbohydrates diet, lack of physical activity and stress, is a trigger for different diseases with complex interactions between the host genetics, the metabolism, the immune system and the microbiota, as the inflammatory bowel disease (IBD), obesity and diabetes. The zebrafish has 70% homology with our genes, shares anatomic and physiological characteristics with mammals, and emerges as a new model for the study of metabolic and inflammatory diseases. In this review, we examine the existing genetic and induced zebrafish models of two important Western diseases with strong inflammatory component, IBD and obesity.Morales Fénero CI, Colombo Flores AA, Câmara NOS. Inflammatory diseases modelling in zebrafish. World J Exp Med 2016; 6(1): 9-20 Available from:

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“…Inflammatory bowel disease includes ulcerative colitis and Crohn's disease; it is one of the most common chronic conditions involving inflammation of the intestine. Numerous animal models of these diseases have been developed, with no single model fully reflecting the pathogenesis observed in humans (Mizoguchi, 2012;Neurath, 2012), suggesting a need for research to improve model relevance. Several models of inflamed intestine have been used to assess NM adhesion and absorption.…”

Section: Models Of Inflammationmentioning

confidence: 99%

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

Venema²,

et al. 2014

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Engineered metal/mineral, lipid and biochemical macromolecule nanomaterials (NMs) have potential applications in food. Methodologies for the assessment of NM digestion and bioavailability in the gastrointestinal tract are nascent and require refinement. A working group was tasked by the International Life Sciences Institute NanoRelease Food Additive project to review existing models of the gastrointestinal tract in health and disease, and the utility of these models for the assessment of the uptake of NMs intended for food. Gastrointestinal digestion and absorption could be addressed in a tiered approach using in silico computational models, in vitro non-cellular fluid systems and in vitro cell culture models, after which the necessity of ex vivo organ culture and in vivo animal studies can be considered. Examples of NM quantification in gastrointestinal tract fluids and tissues are emerging; however, few standardized analytical techniques are available. Coupling of these techniques to gastrointestinal models, along with further standardization, will further strengthen methodologies for risk assessment.

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Animal Models of Inflammatory Bowel Disease

Cited by 225 publications

References 236 publications

Analysing the effect of I1 imidazoline receptor ligands on DSS-induced acute colitis in mice

Analysing the effect of I1 imidazoline receptor ligands on DSS-induced acute colitis in mice

Inflammatory diseases modelling in zebrafish

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

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