Animal models of ischemic heart disease for in vivo cardiac MR imaging research

Feng, Yuanbo; Xie, Yi; Chen, Feng; Li, Junjie; Jin, Lixin; Dymarkowski, Steven; Yu, Jing; Jiang, Yansheng; Peeters, Ronald; Marchal, Guy; Ni, Yicheng

doi:10.1504/ijmic.2010.032809

Cited by 3 publications

(3 citation statements)

References 270 publications

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“…This is the first study where PWI was elaborated at a clinical 3.0T MR scanner to evaluate perfusion deficit in a midsized animal model of MI. Comparing to pigs and dogs, rabbits are of multiple advantages and can provide an ideal animal model for cardiovascular imaging research 29 , 46 - 48 . Rabbit models are beneficial due to their low costs, high reproductivity, and ease of housing and handing during the experiment.…”

Section: Discussionmentioning

confidence: 99%

Towards Stratifying Ischemic Components by Cardiac MRI and Multifunctional Stainings in a Rabbit Model of Myocardial Infarction

Feng¹,

Chen²,

Ma³

et al. 2014

Theranostics

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Objectives: We sought to identify critical components of myocardial infarction (MI) including area at risk (AAR), MI-core and salvageable zone (SZ) by using cardiac magnetic resonance imaging (cMRI) and multifunctional stainings in rabbits.Materials and Methods: Fifteen rabbits received 90-min coronary artery (CA) ligation and reopening to induce reperfused MI. First-pass perfusion weighted imaging (PWI90') was performed immediately before CA reperfusion. Necrosis avid dye Evans blue (EB) was intravenously injected for later MI-core detection. One-day later, cMRI with T2-weighted imaging (T2WI), PWI24h and delayed enhancement (DE) T1WI was performed at a 3.0T clinical scanner. The heart was excised and CA was re-ligated with aorta infused by red-iodized-oil (RIO). The heart was sliced into 3-mm sections for digital radiography (DR), histology and planimetry with myocardial salvage index (MSI) and perfusion density rate (PDR) calculated.Results: There was no significant difference between MI-cores defined by DE-T1WI and EB-staining (31.13±8.55% vs 29.80±7.97%; p=0.74). The AAR was defined similarly by PWI90' (39.93±9.51%), RIO (38.82±14.41%) and DR (38.17±15.98%), underestimated by PWI24h (36.44±5.31%), but overestimated (p<0.01) by T2WI (56.93±8.87%). Corresponding MSI turned out to be 24.17±9.5% (PWI90'), 21.97±9.41% (DR) and 22.68±9.65% (RIO), which were significantly (p<0.01) higher and lower than that with PWI24h (15.15±7.34%) and T2WI (45.52±7.5%) respectively. The PDR differed significantly (p<0.001) between normal myocardium (350.6±33.1%) and the AAR (31.2±15%), suggesting 11-times greater blood perfusion in normal myocardium over the AAR.Conclusion: The introduced rabbit platform and new staining techniques together with the use of a 3.0T clinical scanner for cMRI enabled visualization of MI components and may contribute to translational cardiac imaging research for improved theranostic management of ischemic heart disease.

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Section: Discussionmentioning

confidence: 99%

Towards Stratifying Ischemic Components by Cardiac MRI and Multifunctional Stainings in a Rabbit Model of Myocardial Infarction

Feng¹,

Chen²,

Ma³

et al. 2014

Theranostics

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“…The rabbit appears an ideal animal for cardiac research [1,3,10,27]. Comparing to large animals such as pigs, sheep, and dogs, the mid-size rabbit can be easily handled in addition to its inexpensiveness.…”

Section: Discussionmentioning

confidence: 99%

“…The moderate size rabbit model of occlusion/reperfusion-induced myocardial infarction (MI) has been used in cardiac imaging research [1-3]. In particular, its versatile advantages have been demonstrated in cardiac magnetic resonance imaging (cMRI) studies at a 1.5 T clinic magnet [2].…”

Section: Introductionmentioning

confidence: 99%

Lipomatous metaplasia identified in rabbits with reperfused myocardial infarction by 3.0 T magnetic resonance imaging and histopathology

et al. 2013

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BackgroundCardiac lipomatous metaplasia (LM) occurs in patients with chronic ischemic heart disease and heart failure with unclear mechanisms. We studied coronary occlusion/reperfusion-induced myocardial infarction (MI) in rabbits during a 9-months follow-up using 3.0 T magnetic resonance scanner, and confirmed the presence of MI in acute phase and LM in chronic phase using histopathology.MethodsMI was surgically induced in 10 rabbits by 90-min coronary artery occlusion and reperfusion. Forty-eight hours later, multiparametric cardiac magnetic resonance imaging (cMRI) was performed at a 3.0 T clinical scanner for MI diagnosis and cardiac function analysis. Afterwards, seven rabbits were scarified for histochemical staining with triphenyltetrazolium chloride (TTC), and hematoxylin-eosin (HE), and 3 were scanned with cMRI at 2 days, 2 weeks, 2 months and 9 months for longitudinal observations of morphological and functional changes, and the fate of the animals. Post-mortem TTC, HE and Masson's trichrome (MTC) were studied for chronic stage of MI.ResultsThe size of acute MI correlated well between cMRI and TTC staining (r2=0.83). Global cardiac morphology-function analysis showed significant correlation between increasing acute MI size and decreasing ejection fraction (p<0.001). During 9 months, cMRI documented evolving morphological and functional changes from acute MI to chronic scar transformation and fat deposition with a definite diagnosis of LM established by histopathology.ConclusionsAcute MI and chronic LM were induced in rabbits and monitored with 3.0 T MRI. Studies on this platform may help investigate the mechanisms and therapeutic interventions for LM.

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Bifunctional staining forex vivodetermination of area at risk in rabbits with reperfused myocardial infarction

Feng

Chen

et al. 2013

WJM

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Animal models of ischemic heart disease for in vivo cardiac MR imaging research

Cited by 3 publications

References 270 publications

Towards Stratifying Ischemic Components by Cardiac MRI and Multifunctional Stainings in a Rabbit Model of Myocardial Infarction

Towards Stratifying Ischemic Components by Cardiac MRI and Multifunctional Stainings in a Rabbit Model of Myocardial Infarction

Lipomatous metaplasia identified in rabbits with reperfused myocardial infarction by 3.0 T magnetic resonance imaging and histopathology

Bifunctional staining forex vivodetermination of area at risk in rabbits with reperfused myocardial infarction

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