Anin vivo31P NMR study of the phosphorus metabolites in developing seeds of wheat, soybean and mustard

Abstract

In vivo 31P NMR spectra of wheat, soybean and mustard seeds were recorded during ripening. Signals were detected from phosphomonoesters, cytoplasmic and vacuolar inorganic phosphate (Pi), phytate, nucleoside triphosphate (NTP) and nucleoside diphosphosugars. The spectra of extracts showed an accumulation of phytate during development, accompanied by a decrease in inorganic phosphate, and this was reflected in the in vivo spectra. The intrinsic width of the resonances was a significant obstacle to the interpretation of the in vivo spectra in all three cases, and the problem became more severe with increasing maturity. However, it was still possible to use the chemical shift of the cytoplasmic Pi signal to monitor cytoplasmic pH in both soybean and mustard, and the results provide evidence for the existence of a hypoxic state in developing seeds during the active biosynthetic phase. © 1997 John Wiley & Sons, Ltd.

NMR metabolite profiling analysis reveals changes in phospholipid metabolism associated with the re‐establishment of desiccation tolerance upon osmotic stress in germinated radicles of cucumber

“…2a. Dealing with seeds, we were not surprised to find that the strongest signals were due to phytate molecules, which exhibit the characteristic 1:1:2:2 intensity distribution at neutral pH, and to inorganic Pi at 5.2 ppm (Gambhir, Pande & Ratcliffe 1997). Phytate was abundant in cucumber radicles of all stages (Fig.…”

“…Gly-3-P can be channelled into the glycolytic pathway at the PGA level. Furthermore, this compound and PCho and PEa are intermediary products of phospholipid catabolism that have been detected in NMR profiles of various plant tissues submitted to stress (Spickett et al 1992;Aubert et al 1994;Gambhir et al 1997). Gly-3-P was not detected in non-germinated radicles and it accumulated progressively during radicle growth (Fig.…”

“…The limitations of our NMR approach makes it well possible that metabolites, although important to DT, might have not been detected by our analysis because they are either present at very low concentrations in cucumber radicles or masked by phytate resonance (Fig. 2a; Gambhir et al. 1997).…”

High Resolution 31P NMR Spectroscopy Generates a Quantitative Evolution Profile of Phosphorous Translocation in Germinating Sesame Seed

“…When combined with prudential isotope labelling, solid state NMR spectroscopy has been proven a powerful method for studying the biochemistry of plants in vitro or in vivo , as beautifully illustrated by Schaefer group 20–22 . In the past years, NMR spectroscopy has been employed to investigate the phosphorous fate and phytate dynamics in a variety of systems from soil, manure, poultry litter, foods and fruits to living organisms 23–28 . Shand et al .…”

“…Gambhir et al . 28 studied the phosphorous metabolites of the seeds of wheat, soybean and mustard using liquid state NMR spectroscopy. The cytoplasmic 31 P signals over a range of moisture contents were recorded and examined.…”