Anionic surfactant biodegradability studies by Warburg respirometry

Brink, Robert H.; Meyers, Joseph A.

doi:10.1007/bf02682412

Cited by 3 publications

References 3 publications

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Evaluation of reference carbon and inocula sources used in biodegradation test systems that measure oxygen consumption

Stainken¹,

Stevenson²,

Blum³

1983

Enviro Toxic and Chemistry

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Numerous procedures are used to evaluate the biodegradation potential of organic chemicals. Optimal conditions for tests, efficiency of tests, effects of microbe sources and appropriate reference media often are not defined. This study determined the effect of three inocula [resh activated sludge, yeast (Saccharomyces sp.) and laboratory‐maintained sludge culture] and four reference compounds (glucose, sodium acetate, linear alkylate sulfonate and diethylene glycol) in BOD5, BOD20 (biological oxygen demand) and manometric experiments. The effects of variable nitrogen sources [NH4Cl or (NH2)SO4] in the buffer were examined, and optimal inocula, reference compounds and buffer compositions were determined in each test system. In the BOD5 test using NH4Cl, yeast had the greatest activity, and glucose was the preferred carbon source of all three inocula. When (NH4)2SO4 replaced NH4Cl, fresh sludge had the greatest activity and lowest variability, with sodium acetate being the preferred carbon source. BOD20 tests indicated that fresh sludge and glucose were the optimal combination in buffer with NH4Cl. Manometric results indicated that fresh sludge, sodium acetate and buffer with NH4Cl were the optimal combination.

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