Anisakid Parasites of Fishes Caught on the African Shelf

Abstract

Anisakids of Scomber japonicus, Trachyrincus scabrus, Sardina pilchardus, Merluccius polli, Trachurus trachurus, and Hoplostethus cadenati from African shelf are reported. Parasites were extensively studied with respect to their morphology and sequence analysis of the rDNA fragment (containing internal transcribed spacer [ITS]-1, 5.8S subunit and ITS-2); all of them are approximately 1,000 base pairs. Digestion of the amplified region and its sequencing revealed the presence of a sibling species (A. simplex s.s. and A. pegreffii). Still other sequences represented closely related, but not sibling, species, i.e., Anisakis brevispiculata, A. ziphidarum, and A. physeteris; there were also 2 undescribed anisakid species. These species were temporarily described as Anisakis sp. HC-2005 and Anisakis sp. MP-2005 (found, respectively, in Hoplostethus cadenati and Merluccius polli). The study shows that the parasitic fauna of fishes reflects not only the geographic range of anisakid nematodes but also the feeding behavior of the hosts serving as links in the parasites' life cycles.

Anisakis (Nematoda: Ascaridoidea) from Indonesia

“…Similarly, the genotype Anisakis sp. HC-2005 so far has been reported from 3 different teleost hosts in Indonesia (Sufflamen fraenatum, present study; Lepturacanthus savala, Kuhn et al 2013;and Epinephelus areolatus, Kleinertz et al 2014a), and from northwest Africa (Hoplostethus cadenati from the African shelf, see Kijewska et al 2009), and seems to be not able to extend the host range into the more northern and southern regions. This underlines the importance of low host-specificity to infect a range of first intermediate hosts to extend the host distribution in the anisakid nematodes, as earlier suggested by Kellermanns et al (2007).…”

“…They were then aligned with sequences from other Anisakis species from GenBank for species and genotype identification as follows: A. typica (s.s.) from the spinner dolphin Stenella longirostris from Brazil (AY826724; see Nadler et al 2005), A. simplex C, now A. berlandi from Mirounga angustirostris from California (AY 821739; see Nadler et al 2005), A. pegreffii and A. physeteris from mackerels from Japan (AB277823 and AB277821 respectively; see Umehara et al 2008), and Anisakis sp. HC-2005 from Hoplostethus cadenati from the African shelf (EU718474; see Kijewska et al 2009). We hereby apply the subspecific entity Anisakis typica var.…”

Parasites as biological tags for stock identification of blackspot seabream, <i>Pagellus bogaraveo</i>, in Portuguese northeast Atlantic waters

“…PB-2010, which was not detected elsewhere but showed remarkable genetic similarity, and is probably conspecific with Anisakis sp. HC-2005 detected by Kijewska et al (2009) in fish from the African shelf (Hermida et al 2012). The definitive host of Anisakis sp.…”

Occurrence of anisakid nematodes in Atlantic cod (Gadus morhua) and Greenland cod (Gadus ogac), West Greenland

“…The amplification and digestions products were separated by electrophoresis in respectively 1% routine use agarose (Sigma) and 4% high resolution agarose (Sigma) gel stained with ethidium bromide (10 mg/ml) and visualized under UV light. Both spacers were sequenced in both orientations using the primer sets NC5, NC13 (reverse; 5'-ATCGATGAAGAACGCAGC-3'; Zhu et al 1998) and NC2, Anc13 (forward; 5'-CTTAGTGCTCAATGTGTCTG-3'; Kijewska et al 2009). DNA sequencing was preformed by Macrogen Inc. (Korea).…”