Anisakidae parasitism activated immune response and induced liver fibrosis in wild anadromous Coilia nasus

Abstract

Anisakidae nematode larvae is one of the most common parasites in wild anadromous Coilia nasus. This study aims to explore the mechanism of the C. nasus immune response to the parasitism of Anisakid nematode larvae. Results found that Anisakid nematode larvae parasitism caused liver injury as evidenced by histomorphology results as well as high levels of aminotransferase and aspertate aminotransferase. Furthermore, Anisakid nematode larvae parasitism induced an immune response in the host, which was characterized by the elevated populations of macrophages and neutrophils in the liver and head‐kidney in the Anisakidae‐infected group compared to the noninfected group. The expression of immunoglobulin IgM and IgD in the liver and head‐kidney was also increased in the Anisakidae‐infected group. The Anisakidae‐infected group showed higher activity of antioxidant enzymes catalase and superoxide dismutase, which indicates severe oxidative stress, and increased production of pro‐inflammatory cytokines, TNF‐α, IL‐6 as well as MCP‐1 in the liver compared with the noninfected group. As a result of inflammation, livers of hosts in the Anisakidae‐infected group showed fibrosis, and elevated expression of associated proteins including α‐smooth muscle actin, fibronectin, collagen type I and type III compared with the noninfected group. We demonstrated that Anisakid nematode larvae parasitism results in injury and fibrosis in the liver, and triggers immune cell infiltration and inflammation in the liver and head‐kidney of C. nasus. Altogether, the results provide a foundation for building an interaction between parasite and host, and will contribute to C. nasus population and fishery resource protection.

Ascaridoid Nematodes Infection in Anadromous Fish Coilia nasus from Yangtze River

“…The migration of nematodes would lead to the puncture of the stomach and other organs, which is associated with inflammation and organ damage. Previous studies have demonstrated that ascaridoid infection elicits inflammatory responses and adaptive immunity in C. nasus [28,40,41]. A high nematode infection rate may result in mortality during migration and failure to reach spawning grounds, consequently contributing to a decline in the C. nasus population.…”