Anisotropic analysis of multi‐component T<sub>2</sub> and T<sub>1ρ</sub> relaxations in achilles tendon by NMR spectroscopy and microscopic MRI

Wang, Nian; Xia, Yang

doi:10.1002/jmri.24012

Cited by 41 publications

(33 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Case 2 situation did not occur in this articular cartilage study, but can happen in the studies of nasal cartilage and tendon, both have no complex zonal structures in their morphology [23; 39]. The Case 3 situation occurs in this study when the tissue is soaked in saline, where each of the three components represents each of the three structural zones.…”

Section: Discussionmentioning

confidence: 86%

Experimental issues in the measurement of multi-component relaxation times in articular cartilage by microscopic MRI

Wang

Xia

2013

Journal of Magnetic Resonance

Self Cite

View full text Add to dashboard Cite

A number of experimental issues in the measurement of multi-component T2 and T1ρ relaxations in native and enzymatically digested articular cartilage were investigated by microscopic MRI (μMRI). The issues included the bath solutions (physiological saline and phosphate buffered saline (PBS)), the imaging resolution (35 to 140 μm), the specimen orientations (0° and 55°), and the strength of spin-lock frequencies (0.5 to 2 kHz) in the T1ρ experiments. In addition to cartilage, the samples of agar gel and doped water solution were also used in the investigation. Two imaging sequences were used: CPMG-SE and MSME. All raw data were analyzed by the non-negative least square (NNLS) method. The MSME sequence was shown to result in the observation of multi-component T2, even in the gel and liquid samples, demonstrating the artificial uncleanness of this sequence in the multi-component measurements. The soaking of cartilage in PBS reduced the observable T2 components to one at both 0° and 55°, suggesting the effect of phosphate ions on proton exchange between different pools of water molecules. The cartilage orientation with respect to the external magnetic field and the spin-lock strengths in the T1ρ experiment both affected the quantification of the multi-component relaxation. The transitions between a mono-component and multi-components in cartilage under various experimental conditions call for the extra caution in interpreting the relaxation results.

show abstract

Section: Discussionmentioning

confidence: 86%

Experimental issues in the measurement of multi-component relaxation times in articular cartilage by microscopic MRI

Wang

Xia

2013

Journal of Magnetic Resonance

Self Cite

View full text Add to dashboard Cite

show abstract

“…The different water pools in articular cartilage may have different angular dependent behaviors. The orientation at or near the magic angle can manipulate the exchange process between different pools of water molecules, hence changing the population characteristics for each pool of water molecules 31 . As a result, short and long T2* fractions vary as a function of the angular orientation of the specimen.…”

Section: Discussionmentioning

confidence: 99%

UTE bi-component analysis of T2* relaxation in articular cartilage

Shao

Chang

Pauli

et al. 2016

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

SUMMARY Objectives To determine T2* relaxation in articular cartilage using ultrashort echo time (UTE) imaging and bi-component analysis, with an emphasis on the deep radial and calcified cartilage. Methods Ten patellar samples were imaged using two-dimensional (2D) UTE and Car-Purcell-Meiboom-Gill (CPMG) sequences. UTE images were fitted with a bi-component model to calculate T2* and relative fractions. CPMG images were fitted with a single-component model to calculate T2. The high signal line above the subchondral bone was regarded as the deep radial and calcified cartilage. Depth and orientation dependence of T2*, fraction and T2 were analyzed with histopathology and polarized light microscopy (PLM), confirming normal regions of articular cartilage. An interleaved multi-echo UTE acquisition scheme was proposed for in vivo applications (n = 5). Results The short T2* values remained relatively constant across the cartilage depth while the long T2* values and long T2* fractions tended to increase from subchondral bone to the superficial cartilage. Long T2*s and T2s showed significant magic angle effect for all layers of cartilage from the medial to lateral facets, while the short T2* values and T2* fractions are insensitive to the magic angle effect. The deep radial and calcified cartilage showed a mean short T2* of 0.80 ± 0.05 ms and short T2* fraction of 39.93 ± 3.05% in vitro, and a mean short T2* of 0.93 ± 0.58 ms and short T2* fraction of 35.03 ± 4.09% in vivo. Conclusion UTE bi-component analysis can characterize the short and long T2* values and fractions across the cartilage depth, including the deep radial and calcified cartilage. The short T2* values and T2* fractions are magic angle insensitive.

show abstract

“…Reiter et al 14 identified three exchangeable water components within bovine nasal cartilage on nuclear magnetic resonance (NMR) spectroscopy: water tightly bound to collagen, water tightly bound to proteoglycan, and bulk water with T 2 relaxation times of 2.3 msec, 25.2 msec, and 96.3 msec, respectively. Zheng and Xia 27 and Wang et al 28 described three distinct water components in bovine tendon on NMR spectroscopy with T 2 relaxation times of 3.7 msec, 11.0 msec, and 26.1 msec, which were thought to represent water tightly bound within the triple helical ultrastructure of collagen, water loosely bound to collagen and other macromolecules, and bulk water, respectively. The echo time of the mcDESPOT sequence could not capture signal from the extremely short relaxing water components of cartilage and tendon measured in previous NMR studies.…”

Section: Discussionmentioning

confidence: 99%

Rapid in vivo multicomponent T₂ mapping of human knee menisci

Liu

Samsonov

Wilson

et al. 2015

Magnetic Resonance Imaging

View full text Add to dashboard Cite

Purpose To compare multicomponent T2 parameters of menisci measured using Multicomponent Driven Equilibrium Single Pulse Observation of T1 and T2 (mcDESPOT) in asymptomatic volunteers and osteoarthritis (OA) patients with intact and torn menisci. Materials and Methods The prospective study was performed with Institutional Review Board approval and with all subjects signing written informed consent. mcDESPOT was performed on the knee joint of 12 asymptomatic volunteers and 14 patients with knee OA. Single-component T2 relaxation time (T2Single), T2 relaxation time of the fast relaxing water component (T2F), and the slow relaxing water component (T2S), and fraction of the fast relaxing water component (FF) of the medial and lateral menisci were measured. Multivariate linear regression models were used to compare mcDESPOT parameters between normal menisci in asymptomatic volunteers, intact menisci in OA patients, and torn menisci in OA patients with adjustment for differences in age between subjects. Results The mean mcDESPOT parameters for normal menisci in asymptomatic volunteers, intact menisci in OA patients, and torn menisci in OA patients were respectively 16.1 msec, 18.8 msec, and 22.7 msec for T2Single; 9.0 msec, 10.0 msec, and 11.1 msec for T2F; 24.4 msec, 27.7 msec, and 31.4 msec for T2S; and 34%, 32%, 27% for FF. There were significant differences (P<0.05) in T2Single, T2F, T2S, and FF between the three groups of menisci. Conclusion The menisci of OA patients had significantly higher T2Single, T2F, and T2S and significantly lower FF than normal menisci in asymptomatic volunteers with greater changes in multicomponent T2 parameters noted in torn than intact menisci in OA patients.

show abstract

Anisotropic analysis of multi‐component T₂ and T_1ρ relaxations in achilles tendon by NMR spectroscopy and microscopic MRI

Cited by 41 publications

References 38 publications

Experimental issues in the measurement of multi-component relaxation times in articular cartilage by microscopic MRI

Experimental issues in the measurement of multi-component relaxation times in articular cartilage by microscopic MRI

UTE bi-component analysis of T2* relaxation in articular cartilage

Rapid in vivo multicomponent T₂ mapping of human knee menisci

Contact Info

Product

Resources

About

Anisotropic analysis of multi‐component T2 and T1ρ relaxations in achilles tendon by NMR spectroscopy and microscopic MRI

Cited by 41 publications

References 38 publications

Experimental issues in the measurement of multi-component relaxation times in articular cartilage by microscopic MRI

Experimental issues in the measurement of multi-component relaxation times in articular cartilage by microscopic MRI

UTE bi-component analysis of T2* relaxation in articular cartilage

Rapid in vivo multicomponent T2 mapping of human knee menisci

Contact Info

Product

Resources

About

Anisotropic analysis of multi‐component T₂ and T_1ρ relaxations in achilles tendon by NMR spectroscopy and microscopic MRI

Rapid in vivo multicomponent T₂ mapping of human knee menisci