It is well known that 3D printed parts prepared by fused deposition modeling (FDM) exhibit large anisotropy of mechanical properties. In this article, poly(lactic acid; PLA)/carbon fiber (CF) composites with different built orientations (X, Y, Z) were prepared by FDM. The effects of printing temperature, speed, orientations, and layer thickness on the mechanical properties of the composites were systematically investigated. The mechanical properties of PLA/CF composites show more significant anisotropy. The orientation of the fibers along the printing direction is displayed by scanning electron microscopy. Printing parameters bring almost no effect on mechanical properties of the X‐construct oriented specimen, and bring obvious effect on those of the Y‐construct oriented specimen and Z‐construct oriented specimen. According to the analysis, carbon fiber can amplify this anisotropy from layer fashion, and the key factors from printing parameters are porosity and bond strength between fuses. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48786.