Anisotropic linear media: opto-mechanical analogies between discontinuity surfaces

Bellver-Cebreros, Consuelo; Rodríguez-Danta, Marcelo

doi:10.1364/josaa.36.000089

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…where is the time-averaged Maxwell stress tensor and is the vector pointing from the cylinder’s geometric center to its surface. For an optically anisotropic trapping particle (for quartz, n e = 1.5428 and n o = 1.5341 at 1064 nm (34)), the Maxwell stress tensor can be written as (35)…”

Section: Resultsmentioning

confidence: 99%

Optical Torque Calculations and Measurements for DNA Torsional Studies

Hong,

Ye,

Qian

et al. 2024

Preprint

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The angular optical trap (AOT) is a powerful instrument for measuring the torsional and rotational properties of a biological molecule. Thus far, AOT studies of DNA torsional mechanics have been carried out using a high numerical aperture oil-immersion objective, which permits strong trapping, but inevitably introduces spherical aberrations due to the glass-aqueous interface. However, the impact of these aberrations on torque measurements is not fully understood experimentally, partly due to a lack of theoretical guidance. Here, we present a numerical platform based on the finite element method to calculate forces and torques on a trapped quartz cylinder. We have also developed a new experimental method to accurately determine the shift in the trapping position due to the spherical aberrations by using a DNA molecule as a distance ruler. We found that the calculated and measured focal shift ratios are in good agreement. We further determined how the angular trap stiffness depends on the trap height and the cylinder displacement from the trap center and found full agreement between predictions and measurements. As further verification of the methodology, we showed that DNA torsional properties, which are intrinsic to DNA, could be determined robustly under different trap heights and cylinder displacements. Thus, this work has laid both a theoretical and experimental framework that can be readily extended to investigate the trapping forces and torques exerted on particles with arbitrary shapes and optical properties.SIGNIFICANCEWe developed a simulation platform based on the finite element method for force and torque calculation for particles in an angular optical trap (AOT), with considerations of tightly focused Gaussian beam, spherical aberrations, and optically anisotropic particles. Experimental measurements of focal shift ratio, force, and torque under multiple conditions were in good agreement with predictions from the simulations. We also demonstrated that intrinsic DNA torsional properties can be robustly measured under different AOT measurement conditions, strongly validating our simulations and calibrations. Our platform can facilitate trapping particle design for single-molecule assays using the AOT.

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