Anisotropic, porous hydrogels templated by lyotropic chromonic liquid crystals

Wang, Suitu; Maruri, Daniel P.; Boothby, Jennifer M.; Lu, Xili; Rivera‐Tarazona, Laura K.; Varner, Victor D.; Ware, Taylor H.

doi:10.1039/d0tb00904k

Cited by 14 publications

(15 citation statements)

References 58 publications

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“…Samples were immersed in PBS buffer at 37°C for 24 h to swell fully and then lyophilized overnight; after that, the cross-sections of dried hydrogels were prepared using a blade and coated with a thin layer of sputtered gold for 120 s. The cross-sectional morphology of the hydrogel samples was determined using a scanning electron microscope (JSM-5900, JEOL, Japan). ImageJ software (Nation Institutes of Health, Bethesda, MD, USA) was used to analyze the pore size and distribution through SEM images. , …”

Section: Methodsmentioning

confidence: 99%

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Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering

Choi

Jang

et al. 2021

ACS Omega

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Gelatin methacryloyl (GelMA) has been widely studied as a biomaterial for tissue engineering. Most studies focus on mammalian gelatin, but certain factors, such as mammalian diseases and diet restrictions, limit the use of mammalian gelatin. Thus, fish gelatin has received much attention as a substitute material in recent years. To develop a broadly applicable hydrogel with excellent properties, an interpenetrating polymer network (IPN) hydrogel was synthesized, since IPN hydrogels consist of at least two different hydrogel components to combine their advantages. In this study, we prepared GelMA using type A and fish gelatin and then synthesized IPN hydrogels using GelMA with alginate. GelMA singlenetwork hydrogels were used as a control group. The favorable mechanical properties of type A and fish hydrogels improved after the synthesis of the IPN hydrogels. Type A and fish IPN hydrogels showed different mechanical properties (mechanical strength, swelling ratio, and degradation rate) and different cross-sectional morphologies, since the degree of mechanical enhancement in fish IPN hydrogels was less than that in type A; however, the cell biocompatibilities were not significantly different. Therefore, these findings could serve as a reference for future studies when selecting GelMA as a biological material for tissue engineering.

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Section: Methodsmentioning

confidence: 99%

“…ImageJ software (Nation Institutes of Health, Bethesda, MD, USA) was used to analyze the pore size and distribution through SEM images. 37,38 2.4. Compressive Modulus Test.…”

Section: Cross-sectional Morphologymentioning

confidence: 99%

Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering

Choi

Jang

et al. 2021

ACS Omega

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“…Nuclear alignment on different micropatterns of aligned collagen fibrils and control was computed using "Fit Ellipse" in ImageJ software as outlined previously. 28 The angle between the major axis of the nuclei that fitted into an ellipse and the horizontal axis of the image was measured. These angles were used to measure the orientation index (OI) as an indicator of the overall cell alignment with 100% OI indicating alignment of the nucleus with the underlying fibrils (i.e., along the horizontal).…”

Section: Coating Of Pdms-coated Glass Coverslips With Monomericmentioning

confidence: 99%

Fabrication of Micropatterns of Aligned Collagen Fibrils

Subramanian,

Tjahjono,

Hernandez

et al. 2024

Langmuir

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Many tissues in vivo contain aligned structures such as filaments, fibrils, and fibers, which expose cells to anisotropic structural and topographical cues that range from the nanometer to micrometer scales. Understanding how cell behavior is regulated by these cues during physiological and pathological processes (e.g., wound healing, cancer invasion) requires substrates that can expose cells to anisotropic cues over several length scales. In this study, we developed a novel method of fabricating micropatterns of aligned collagen fibrils of different geometry onto PDMS-coated glass coverslips that allowed us to investigate the roles of topography and confinement on corneal cell behavior. When corneal cells were cultured on micropatterns of aligned collagen fibrils in the absence of confinement, the degree of cell alignment increased from 40 ± 14 to 82 ± 5% as the size of the micropattern width decreased from 750 to 50 μm. Although the cell area (∼2500 μm 2 ), cell length (∼160 μm), and projected nuclear area (∼175 μm 2 ) were relatively constant on the different micropattern widths, cells displayed an increased aspect ratio as the width of the aligned collagen fibril micropatterns decreased. We also observed that the morphology of cells adhering to the surrounding uncoated PDMS was dependent upon both the size of the aligned collagen fibril micropattern and the distance from the micropatterns. When corneal cells were confined to the micropatterns of aligned collagen fibrils by a Pluronic coating to passivate the surrounding area, a similar trend in increasing cell alignment was observed (35 ± 10 to 89 ± 2%). However, the projected nuclear area decreased significantly (∼210 to 130 μm 2 ) as the micropattern width decreased from 750 to 50 μm. The development of this method allows for the deposition of aligned collagen fibril micropatterns of different geometries on a transparent and elastic substrate and provides an excellent model system to investigate the role of anisotropic cues in cell behavior.

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“…Various hydrogel structures are obtained by harnessing LCLC phases, obtained by just varying polymerization temperatures. 34…”

Section: Molecular Design Of Lyotropic Chromonic Liquid Crystalline Materialsmentioning

confidence: 99%

Recent progress in functional materials from lyotropic chromonic liquid crystals

Bosire

Ndaya

Kasi

2020

Polymer International

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Research on lyotropic chromonic liquid crystals has grown tremendously from fundamental studies to designing functional materials for applications in biological fields and other technological areas. The majority of the research has centered on utilizing chromonic small molecules predominantly dissolved in aqueous solvents. But these often present processing difficulties and a host of other unattractive features including poor mechanical and chemical stabilities. Mesophase properties of chromonic liquid crystals as characterized using X-ray diffraction and scattering techniques, spectroscopy and light microscopy are explored. We review potential applications and future perspectives of this subfield where methods of transforming chromonic molecules into functional materials are highlighted.

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Anisotropic, porous hydrogels templated by lyotropic chromonic liquid crystals

Abstract:
Approaches to control the microstructure of hydrogels enable the control of cell–material interactions and the design of stimuli-responsive materials.

Cited by 14 publications

References 58 publications

Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering

Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering

Fabrication of Micropatterns of Aligned Collagen Fibrils

Recent progress in functional materials from lyotropic chromonic liquid crystals

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Anisotropic, porous hydrogels templated by lyotropic chromonic liquid crystals

Abstract: Approaches to control the microstructure of hydrogels enable the control of cell–material interactions and the design of stimuli-responsive materials.

Cited by 14 publications

References 58 publications

Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering

Mammalian and Fish Gelatin Methacryloyl–Alginate Interpenetrating Polymer Network Hydrogels for Tissue Engineering

Fabrication of Micropatterns of Aligned Collagen Fibrils

Recent progress in functional materials from lyotropic chromonic liquid crystals

Contact Info

Product

Resources

About

Abstract:
Approaches to control the microstructure of hydrogels enable the control of cell–material interactions and the design of stimuli-responsive materials.