Anisotropic silk nanofiber layers as regulators of angiogenesis for optimized bone regeneration

Fan, Zhihai; Liu, Hongxiang; Shi, Shilei; Ding, Zhaozhao; Zhang, Zhen; Lü, Qiang; Kaplan, David L.

doi:10.1016/j.mtbio.2022.100283

Cited by 10 publications

(22 citation statements)

References 52 publications

(90 reference statements)

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“…Higher blood vessel density appeared in the SNF6-D group at week 4 and then in the SNF10-D group at week 8. Similar to our recent study, [18] the more sustained release of DFO, the better the angiogenesis outcomes at the defect site. The quality of the regenerated bones was also improved in the DFO-laden cryogel groups (Figure 8).…”

Section: Bone Regeneration and Vascularization In Vivosupporting

confidence: 90%

“…Previous studies revealed that DFO facilitated the osteodifferentiation. [18,[56][57][58][59][60] Compared to DFO-free cryogels, the DFO-laden cryogels with same silk concentrations supported higher expression of RUNX2, suggesting synergistic action of DFO and mechanical cues in the cryogels. Thus, the highest RUNX2 expression was achieved in the SNF10-D group (Figure 5b).…”

Section: In Vitro Osteogenic Differentiation Of Rbmscs On Cryogelsmentioning

confidence: 99%

“…Our recent study revealed that dynamic angiogenesis was achieved through sustained release of DFO to optimize the osteogenesis capacity. [18] The sections collected at 4 and 8 weeks after implantation were stained with CD 31 and 𝛼-smooth muscle actin (𝛼-SMA) to visualize angiogenesis (Figure 7a). The DFO-free groups showed inferior angiogenic stimulating action and maintained low blood vessel density at weeks 4 and 8 after surgery.…”

Section: Bone Regeneration and Vascularization In Vivomentioning

confidence: 99%

“…[12][13][14][15] In particular, sustained and dynamic osteogenesis and angiogenesis were critical determinants in the bone repair process. [16][17][18] Bioactive drugs, growth factors, and stem cells were loaded into cryogels to provide multiple chemical and biological cues to facilitate bone regeneration. [5,19] However, growth factors and cytokines are prone to rapid inactivation, while small drugs are often released rapidly from cryogels.…”

Section: Introductionmentioning

confidence: 99%

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Tough Porous Silk Nanofiber‐Derived Cryogels with Osteogenic and Angiogenic Capacity for Bone Repair

Hou

Ding

Zheng

et al. 2023

Adv Healthcare Materials

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Tough porous cryogels with angiogenesis and osteogenesis features remain a design challenge for utility in bone regeneration. Here, building off of the recent efforts to generate tough silk nanofiber‐derived cryogels with osteogenic activity, deferoxamine (DFO) is loaded in silk nanofiber‐derived cryogels to introduce angiogenic capacity. Both the mechanical cues (stiffness) and the sustained release of DFO from the gels are controlled by tuning the concentration of silk nanofibers in the system, achieving a modulus above 400 kPa and slow release of the DFO over 60 days. The modulus of the cryogels and the released DFO induce osteogenic and angiogenic activity, which facilitates bone regeneration in vivo in femur defects in rat, resulting in faster regeneration of vascularized bone tissue. The tunable physical and chemical cues derived from these nanofibrous‐microporous structures support the potential for silk cryogels in bone tissue regeneration.

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Section: Bone Regeneration and Vascularization In Vivosupporting

confidence: 90%

Section: In Vitro Osteogenic Differentiation Of Rbmscs On Cryogelsmentioning

confidence: 99%

Section: Bone Regeneration and Vascularization In Vivomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tough Porous Silk Nanofiber‐Derived Cryogels with Osteogenic and Angiogenic Capacity for Bone Repair

Hou

Ding

Zheng

et al. 2023

Adv Healthcare Materials

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“…In this review, we are more focused on its unique application in the fields of cell-material interactions, soft tissue engineering, and flexible bioelectronic devices. As for the other important biomedical applications, such as wound dressing, hard tissue engineering and drug delivery etc ., extensive descriptions could be seen from previous publications [ 28 , 29 , [109] , [110] , [111] , [112] , [113] , [114] , [115] , [116] , [117] ].…”

Section: Advanced Biomedical Applications Of Rsf Materialsmentioning

confidence: 99%

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Yao

Zou

Fan

et al. 2022

Materials Today Bio

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Simulation of Cortical and Cancellous Bone to Accelerate Tissue Regeneration

Fan

Liu

Ding

et al. 2023

Adv Funct Materials

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Different tissues have complex anisotropic structures to support biological functions. Mimicking these complex structures in vitro remains a challenge in biomaterials designs. Here, inspired by different types of silk nanofibers, a composite materials strategy is pursued toward this challenge. A combination of fabrication methods is utilized to achieve separate control of amorphous and beta‐sheet rich silk nanofibers in the same solution. Aqueous solutions containing two types of silk nanofibers are simultaneously treated with an electric field and with ethylene glycol diglycidyl ether (EGDE). Under these conditions, the beta‐sheet rich silk nanofibers in the mixture responded to the electric field while the amorphous nanofibers are active in the crosslinking process with the EGDE. As a result, cryogels with anisotropic structures are prepared, including mimics for cortical‐ and cancellous‐like bone biomaterials as a complex osteoinductive niche. In vitro studies revealed that mechanical cues of the cryogels induced osteodifferentiation of stem cells while the anisotropy inside the cryogels influenced immune reactions of macrophages. These bioactive cryogels also stimulated improved bone regeneration in vivo through modulation of inflammation, angiogenesis and osteogenesis responses, suggesting an effective strategy to develop bioactive matrices with complex anisotropic structures beneficial to tissue regeneration.

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Anisotropic silk nanofiber layers as regulators of angiogenesis for optimized bone regeneration

Cited by 10 publications

References 52 publications

Tough Porous Silk Nanofiber‐Derived Cryogels with Osteogenic and Angiogenic Capacity for Bone Repair

Tough Porous Silk Nanofiber‐Derived Cryogels with Osteogenic and Angiogenic Capacity for Bone Repair

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Simulation of Cortical and Cancellous Bone to Accelerate Tissue Regeneration

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