We present new, densely sampled shear-wave splitting results from southern Minnesota and adjacent areas of neighboring states, sampling the southwestern limit of the Archean Superior Province and straddling the Proterozoic Mid-Continent Rift (MCR). The new measurements include data from the Earthscope Transportable Array (TA) as well as the Superior Province Rifting Earthscope Experiment (SPREE), yielding 99 new station-averaged measurements. The split times show a consistent decrease from 1.1 s in the NE to 0.2 s in the SW, with the lowest values being associated with the Minnesota River Valley Terrane (MRVT). From modeling and other geophysical constraints, we interpret the split time variations to represent variations in fabric strength within a thick lithosphere, rather than lithospheric thinning or a multi-layered effect, and propose that the weak fabric of the MRVT is associated with a different mechanism of formation than elsewhere in the Superior Province. The fast directions we measure range from NNE-SSW to E-W and vary on a shorter length scale than the split times, with a pattern of NE-SW splits that closely follows the axis of the MCR. We interpret this as a perturbation of the net fast direction due to anisotropy in an underplate along the rift.Plain Language Summary This study presents new measurements of oriented fabric in the Earth's lithosphere, detected from variations in the polarization of seismic waves from distant earthquakes. These results are from southern Minnesota and the surrounding area, combining results from the national grid of Earthscope Transportable Array instruments with a denser deployment of portable instruments. The dense deployment was located so as to examine the Mid-Continent Rift (MCR), a billion-year-old region where stretching of the tectonic plate caused large amounts of volcanic rock to erupt. We found that the strength of fabric was largely unaffected by the MCR, even within the rift's boundaries, and instead varies according to the much older (around two and a half billion years old) regions predating it. The direction of the fabric, however, is influenced by the rift, with a consistent change in orientation that follows the rift axis. We interpret this effect as resulting from a layer of igneous rock that formed at the base of the crust during the rifting process. FREDERIKSEN ET AL.