ANKK1/DRD2 gene Taq1A polymorphism (rs1800497) as a possible genetic marker of food-addiction-related eating disturbances in overweight patients

Abstract

Controlling the epidemic of overweight and obesity is one of the major challenges to modern healthcare. One of the possible causes of overweight and obesity can be food addiction manifesting as overeating and other eating disturbances (ED).We conducted a cross-sectional study to test the association between addiction-related ANKK1/DRD2 gene Taq1A polymorphism (rs1800497) and ED in overweight patients. Overall, 527 outpatients (469 (89,0%) females, 58 (11,0%) males; mean ± SD: BMI — 35,8±7,3 kg/m2; age — 46,7±11,8 years) of European ancestry were included in the study. Eating Disorder Examination — Questionnaire (EDE-Q), Eating Attitudes Test (EAT-26) and Dutch Eating Behavior Questionnaire (DEBQ) were used to assess the ED. We also used Hospital Anxiety and Depression Scale (HADS), Hamilton Rating Scale for Depression (HRDS), Hamilton Anxiety Rating Scale (HARS) and Yale-Brown Obsessive-Compulsive Scale (YBOCS) to assess depressive, anxiety and obsessive-compulsive disturbances respectively. DNA was extracted from blood samples and polymorphism rs1800497 was detected by RT-PCR.According to the dominant genetic model, carriers of minor T allele (“CT+TT” group) in contrast to wild-type allele homozygous patients (“CC” group) were younger (p=0,075) and demonstrated higher scores of EDE-Q (p=0,085) and emotional eating subscale of DEBQ (p=0,063). Moreover, among them the proportion of patients with high ED risk was significantly increased vs. CC group: 1) EAT-26 score: 15,2% vs. 9,0%, OR=1,82 (CI95% (1,054-3,134), p=0,03; 2) EDE-Q score: 46,6% vs. 37,5%, OR=1,45 (CI95% (1,015-2,072), p=0,041. BMI was correlated with emotional eating subscale of DEBQ score in “CT+TT” group only (ρs =0,199, p=0,004). Correlation analysis showed weaker connections between ED and affective disturbances in “CT+TT” group.Our results show that carriers of the addiction-related T allele of ANKK1/DRD2 gene Taq1A polymorphism comprise the at-risk group for eating disturbances in overweight individuals. This may support the role of food addiction in overweight and obesity.

Working Memory Gating in Obesity: Insights from a Case-Control fMRI Study

“…Critically, carrying the A-allele has been linked to reduced striatal D2 receptor density (Thompson et al, 1997; Pohjalainen et al, 1998; Jönsson et al, 1999; Eisenstein et al, 2016) and is often considered a risk factor for obesity (Cardel et al, 2019, Kibitov et al, 2020, Johnson & Kenny, 2010) or other addictive-like behaviors (Smith et al, 2008; Nacak et al, 2012; Comings & Blum, 2000, Gorwood et al, 2012). Of course, we cannot say what, if any, came first - dopamine alterations or obesity.…”