Annelida: Environmental Interactions and Ecotoxicity in Relation to the Earthworm Immune System

Roubalová, Radka; Płytycz, Barbara; Procházková, Petra; Pacheco, Natividad Isabel Navarro; Bilej, Martin

doi:10.1007/978-3-319-76768-0_27

Cited by 4 publications

(3 citation statements)

References 123 publications

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“…In the event of excessive ingestion of Ag that can be not managed conventionally by storage in proteins and excretion, another mechanism might help limit the levels of Ag in the body. For example, Roubalová et al (2018) showed that when earthworms are confronted by aggression, earthworms can expel coelomic fluid with coelomocytes via the dorsal pores. This mechanism would quickly remove from the body a large amount of metals trapped in the coelomocytes.…”

Section: Discussionmentioning

confidence: 99%

Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida

Courtois¹,

Rorat²,

Lemière³

et al. 2020

Environ Sci Pollut Res

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The use of silver nanoparticles (AgNPs) in agriculture and many consumer products has led to significant release of Ag in the environment. Although Ag toxicity in terrestrial organisms has been studied extensively, very little is known about the accumulation capacity and coping mechanisms of organisms in Agcontaminated soil. In this context, we exposed Eisenia fetida earthworms to artificial OECD soil spiked with a range of concentrations of Ag (AgNPs or AgNO 3 ). The main aims were to (1) identify the location and form of accumulation of Ag in the exposed earthworms and (2) better understand the physiological mechanisms involved in Ag detoxification. The results showed that similar doses of AgNPs or AgNO 3 did not have the same effect on E. fetida survival. The two forms of Ag added to soil exhibited substantial differences in speciation at the end of exposure, but the Ag speciation and content of Ag in earthworms were similar, suggesting that biotransformation of Ag occurred. Finally, 3D images of intact earthworms obtained by X-ray micro-computed tomography revealed that Ag accumulated preferentially in the chloragogen tissue, coelomocytes and nephridial epithelium.Thus, E. fetida bioaccumulates Ag, but a regulation mechanism limit its impact in a very efficient manner. The location of Ag in the organism, the competition between Ag and Cu, and the speciation of internal Ag suggest a link between Ag and the thiol-rich proteins that are widely present in these tissues, most probably metallothioneins, which are key proteins in the sequestration and detoxification of metals.

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Section: Discussionmentioning

confidence: 99%

Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida

Courtois¹,

Rorat²,

Lemière³

et al. 2020

Environ Sci Pollut Res

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“…The uniformly reddish Eisenia andrei (Ea) and striped 'tiger worms' E. fetida (Ef) are widely used in toxicology, ecotoxicology, ecology, environmental studies [1,2] and in biomedicine as a source of bioactive molecules [3,4], all of which require accurate species identification. However the existence of hybrids was revealed by mixed patterns of electromorphs of esterase activity in laboratory mated specimens [5] and then relicts of past hybridization between Ea and Ef were recognized in natural populations from Scandinavia [6].…”

Section: Introductionmentioning

confidence: 99%

Polymorphic microsatellite markers demonstrate hybridization and interspecific gene flow between lumbricid earthworm species, Eisenia andrei and E. fetida

et al. 2022

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The lumbricid earthworms Eisenia andrei (Ea) and E. fetida (Ef) have been used as model organisms for studies on hybridization. Previously they have been identified by species specific sequences of the mitochondrial COI gene of maternal origin (‘a’ or ‘f’) and the nuclear 28S gene of maternal/paternal origin (‘A’ or ‘F’). In experimental crosses, these hermaphroditic species produce progeny of genotypes Ea (aAA), Ef (fFF) and hybrids (aAF and fFA) originating by self-fertilization or cross-fertilization. To facilitate studies on new aspects of the breeding biology and hybridization of earthworms, polymorphic microsatellite markers were developed based on 12 Ea and 12 Ef specimens and validated on DNA samples extracted from 24 genotyped specimens (aAA, fFF, aAF and fFA) from three laboratory-raised families and 10 of them were applied in the present study. The results indicate that microsatellite markers are valuable tools for tracking interspecific gene flow between these species.

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“…This is primarily due to the fact that the main criterion of distribution is morphological characters, which are interpreted in different ways by scientists. Confusion still arises because of the terms, because in the outdated literature such a concept as "leukocytes" is used, which is not entirely true [3]. Factors of the human immune system are important bioindicators of exogenous influences [4][5][6][7].…”

mentioning

confidence: 99%

Identification of Lumbricus Terrestris Immune Cells

Sokolenko¹,

Sokolenko²,

Zubenko³

2021

Theoretical and Empirical Scientific Research: Concept and Trends Volume1

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Annelida: Environmental Interactions and Ecotoxicity in Relation to the Earthworm Immune System

Cited by 4 publications

References 123 publications

Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida

Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida

Polymorphic microsatellite markers demonstrate hybridization and interspecific gene flow between lumbricid earthworm species, Eisenia andrei and E. fetida

Identification of Lumbricus Terrestris Immune Cells

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