Annexin-A1 protein and its relationship to cortisol in human saliva

Fowkes, Robert C.; Moradi-Bidhendi, Niloufar; Branceleone, Vincenzo; Zariwala, Mohammed Gulrez; Brady, Dorothy S.; Jessop, David S.; Perretti, Mauro; Renshaw, Derek

doi:10.1016/j.psyneuen.2012.08.003

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…On arrival at the study center the samples were re-bagged and labelled with the dog’s study ID, and stored in a refrigerator at −20°C until the day of the assay. Cortisol levels in the samples were determined with a commercial salivary cortisol assay kit and performed according to methods described previously (Salimetrics, Newmarket, UK; [30, 31]). Briefly, samples were first finely cut into short sections to prevent tangling during pulverization and weighed.…”

Section: Methodsmentioning

confidence: 99%

What can we learn from the hair of the dog? Complex effects of endogenous and exogenous stressors on canine hair cortisol

et al. 2019

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Hair is an emerging biological matrix in which to measure chronic HPA axis activity, offering a longer term view into an animal’s life. We explored effects of exogenous (e.g. lifestyle, medications, social environment) and endogenous (e.g. disease, behaviour) stressors on hair cortisol concentration (HCC) in a population of Border Collies (BCs). Owners of BCs were recruited and reported their dog’s lifestyle, clinical history, anxiety-related behaviour, and collected a white hair sample from their dog’s dorsal neck region. HCC was determined using established methods with a commercial cortisol assay kit. Samples from 135 BCs were analysed, with 91 healthy controls and 44 diagnosed with epilepsy as a model disease. Factors associated with higher HCC included psychosocial stressors (living with three or more other dogs) and lifestyle (engaging in competitive flyball); while factors associated with lower HCC included anxiety (stranger-directed and non-social), health (epilepsy diagnosis, with number of seizures to date negatively correlated with HCC) and medication (certain anti-epileptic drugs were associated with elevated or reduced HCC). These novel results highlight the potential of chronic stress with frequent or persisting HPA-axis hyperactivity leading to a state of hypocortisolism, and the need to consider stressor recency and recurrence when interpreting HCC data.

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Section: Methodsmentioning

confidence: 99%

What can we learn from the hair of the dog? Complex effects of endogenous and exogenous stressors on canine hair cortisol

et al. 2019

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“…Cortisol in the samples was determined with a commercial salivary cortisol assay kit (Salimetrics, Newmarket, UK) (Fowkes et al 2013, Meyer et al 2014. Briefly, 2-3mg of whole hair shafts were cut finely then immersed in 2ml of absolute methanol (18 h, 20 o C) before centrifugation (3000 g, 5 min, 4 o C).…”

Section: Cortisol Assaymentioning

confidence: 99%

WIND TURBINES CAUSE CHRONIC STRESS IN BADGERS (MELES MELES) IN GREAT BRITAIN

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Smith

Fowkes

2016

Journal of Wildlife Diseases

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There is a paucity of data with which to assess the effects of wind turbines noise on terrestrial wildlife, despite growing concern about the impact of infrasound from wind farms on human health and wellbeing. The study was undertaken to assess whether the presence of turbines impact the stress levels of badgers in nearby setts. This was achieved by using their hair cortisol level to determine if the badgers are physiological stressed. The results reveal that the hair of badgers living within 1 km of a wind farm have a 264% higher cortisol level than control badgers. This demonstrates that affected badgers suffer from enhanced hypothalamo-pituitary-adrenal activity and thus are physiologically stressed. No differences were found between the cortisol levels of badgers living near wind farms operational since 2009 and 2012, indicating that the animals do not become habituated to turbine disturbance. Cortisol levels in the affected badgers did not vary in relation to the distance from turbines within 1km, wind farm annual power output or number of turbines. We propose that the rise in cortisol levels in affected badgers is caused by the turbines' sound. This could have detrimental impacts on the animals' immune system which could result in the increased risk of infection and disease and thus facilitating the spread of disease within the badger population.

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“…It was described as anti-inflammatory protein and to be positively regulated by cortisol in saliva. 45 Cortisol is known to increase with age, thus leading to higher salivary concentrations of ANXA1 and other proteins regulated by the glucocorticoid. 46 Neutrophil gelatinase-associated lipocalin (NGAL) was highly associating and increasing with age in our analysis.…”

Section: ■ Discussionmentioning

confidence: 99%

Cross-Sectional Association of Salivary Proteins with Age, Sex, Body Mass Index, Smoking, and Education

et al. 2017

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Whole saliva is gaining more and more attention as a diagnostic tool to study disease-specific changes in human subjects. Prior to the actual disease-related analyses, it is important to understand the influence of various demographic variables and coupled phenotypes on salivary protein signatures. In a cross-sectional approach, we analyzed the influence of age, sex, body mass index (BMI), smoking, and education on salivary protein signatures in whole saliva samples of 187 individuals. Subjects were randomly selected from the population-based Study of Health in Pomerania (SHIP-Trend). Stimulated whole saliva was collected, and proteins were precipitated and proteolytically digested. Samples were analyzed by label-free tandem mass spectrometry. Of the 602 human proteins identified in at least 40% of the saliva samples, we used 304 proteins, which could be identified with at least two unique peptides, for statistical analyses. Univariate and multivariate linear models were used to reveal associations with the phenotypes. The largest number of proteins was associated with smoking status. Moreover, age had a distinct influence on the salivary protein composition. The study discloses the influence of common phenotypes on the salivary protein pattern of human subjects. These results should be considered when studying disease-related proteome signatures in saliva.

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Annexin-A1 protein and its relationship to cortisol in human saliva

Cited by 8 publications

References 21 publications

What can we learn from the hair of the dog? Complex effects of endogenous and exogenous stressors on canine hair cortisol

What can we learn from the hair of the dog? Complex effects of endogenous and exogenous stressors on canine hair cortisol

WIND TURBINES CAUSE CHRONIC STRESS IN BADGERS (MELES MELES) IN GREAT BRITAIN

Cross-Sectional Association of Salivary Proteins with Age, Sex, Body Mass Index, Smoking, and Education

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