Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies

Fang, Yi; Lin, Chiou Feng; Liao, Pao Chi; Kuo, Yu Min; Wang, Shuying; Yeh, Trai Ming; Shieh, Chi Chang; Su, Ih Jen; Lei, Huan Yao; Lin, Yee Shin

doi:10.1016/j.molimm.2009.11.019

Cited by 37 publications

(59 citation statements)

References 78 publications

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“…Further, they observed that RV colocalizes with Src, PI3K, and Akt in membrane rafts. These and several related findings demonstrate that lipid raft-mediated signaling pathways are regulated during viral infections and play critical role in disease pathogenesis (Bentley et al 2007a;Silva et al 2011;Ebihara et al 2008;Huang et al 2009b;Fang et al 2010) (Fig. 2).…”

Section: Blue Tongue Virussupporting

confidence: 61%

Unraveling the role of membrane microdomains during microbial infections

Bagam

Singh

Inda³

et al. 2017

Cell Biol Toxicol

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Infectious diseases pose major socioeconomic and health-related threats to millions of people across the globe. Strategies to combat infectious diseases derive from our understanding of the complex interactions between the host and specific bacterial, viral, and fungal pathogens. Lipid rafts are membrane microdomains that play important role in life cycle of microbes. Interaction of microbial pathogens with host membrane rafts influences not only their initial colonization but also their spread and the induction of inflammation. Therefore, intervention strategies aimed at modulating the assembly of membrane rafts and/or regulating raft-directed signaling pathways are attractive approaches for the. management of infectious diseases. The current review discusses the latest advances in terms of techniques used to study the role of membrane microdomains in various pathological conditions and provides updated information regarding the role of membrane rafts during bacterial, viral and fungal infections.

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Section: Blue Tongue Virussupporting

confidence: 61%

Unraveling the role of membrane microdomains during microbial infections

Bagam

Singh

Inda³

et al. 2017

Cell Biol Toxicol

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“…Other investigators have reported that IL-6, IFN-␥, and vascular endothelial growth factor can upregulate annexin II synthesis in epithelial cells. 28,29 These peptides play critical roles in the pathogenesis of lupus nephritis 30 -32 and are synthesized by both infiltrating and resident renal cells. Interstitial inflammation, which is commonly observed in lupus nephritis, 33 may thus account for increased tubulointerstitial annexin II expression.…”

Section: Discussionmentioning

confidence: 99%

Anti-dsDNA Antibodies Bind to Mesangial Annexin II in Lupus Nephritis

Yung

Cheung

Zhang

et al. 2010

Journal of the American Society of Nephrology

148

144

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Production of anti-dsDNA antibodies is a hallmark of lupus nephritis, but how these antibodies deposit in organs and elicit inflammatory damage remains unknown. In this study, we sought to identify antigens on the surface of human mesangial cells (HMC) that mediate the binding of human anti-dsDNA antibodies and the subsequent pathogenic processes. We isolated anti-dsDNA antibodies from patients with lupus nephritis by affinity chromatography. We used multiple methods to identify and characterize antigens from the plasma membrane fraction of mesangial cells that crossreacted with the anti-dsDNA antibodies. We found that annexin II mediated the binding of anti-dsDNA antibodies to HMC. After binding to the mesangial cell surface, anti-dsDNA antibodies were internalized into the cytoplasm and nucleus. This also led to induction of IL-6 secretion and annexin II synthesis, mediated through activation of p38 MAPK, JNK, and AKT. Binding of anti-dsDNA antibodies to annexin II correlated with disease activity in human lupus nephritis. Glomerular expression of annexin II correlated with the severity of nephritis, and annexin II colocalized with IgG and C3 deposits in both human and murine lupus nephritis. Gene silencing of annexin II in HMC reduced binding of anti-dsDNA antibody and partially decreased IL-6 secretion. In summary, our data demonstrate that annexin II mediates the binding of anti-dsDNA antibodies to mesangial cells, contributing to the pathogenesis of lupus nephritis. This interaction provides a potential target for therapeutic intervention.

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“…At the cellular level, annexin A2 is expressed on endothelial cell surfaces and acts as a co-receptor for plasminogen and tissue plasminogen activator (20). Furthermore, annexins are commonly dysregulated in cancer (21) and annexin A2 is upregulated in a variety of tumors and cancer cell lines (22,23).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profile during Chondrogenesis in Human Bone Marrow derived Mesenchymal Stem Cells using a cDNA Microarray

et al. 2011

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Mesenchymal stem cells (MSCs) have the capacity to proliferate and differentiate into multiple connective tissue lineages, which include cartilage, bone, and fat. Cartilage differentiation and chondrocyte maturation are required for normal skeletal development, but the intracellular pathways regulating this process remain largely unclear. This study was designed to identify novel genes that might help clarify the molecular mechanisms of chondrogenesis. Chondrogenesis was induced by culturing human bone marrow (BM) derived MSCs in micromass pellets in the presence of defined medium for 3, 7, 14 or 21 days. Several genes regulated during chondrogenesis were then identified by reverse transcriptase-polymerase chain reaction (RT-PCR). Using an ABI microarray system, we determined the differential gene expression profiles of differentiated chondrocytes and BM-MSCs. Normalization of this data resulted in the identification of 1,486 differentially expressed genes. To verify gene expression profiles determined by microarray analysis, the expression levels of 10 genes with high fold changes were confirmed by RT-PCR. Gene expression patterns of 9 genes (Hrad6B, annexinA2, BMP-7, contactin-1, peroxiredoxin-1, heat shock transcription factor-2, synaptotagmin IV, serotonin receptor-7, Axl) in RT-PCR were similar to the microarray gene expression patterns. These findings provide novel information concerning genes involved in the chondrogenesis of human BM-MSCs.

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Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies

Cited by 37 publications

References 78 publications

Unraveling the role of membrane microdomains during microbial infections

Unraveling the role of membrane microdomains during microbial infections

Anti-dsDNA Antibodies Bind to Mesangial Annexin II in Lupus Nephritis

Gene Expression Profile during Chondrogenesis in Human Bone Marrow derived Mesenchymal Stem Cells using a cDNA Microarray

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