Annexin V FITC conjugated as a radiation toxicity indicator in lymphocytes following radiation overexposure in radiotherapy programs

Tavakoli, Mahdi; Kheirollahi, Majid; Kiani, Ali Asghar; Kazemi, Mohammad; Javanmard, Shaghayegh Haghjooy; Mohebat, Leili

doi:10.4103/2277-9175.158025

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…Detection of fluorescence signals was under the fluorescence microscope (BX51, Olympus, Japan). 15 Fluorescence Intensity Analysis. All of the photos of the control and treated granulosa cells were from the same slide and scanning settings.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

Wang

Luo

et al. 2018

J. Agric. Food Chem.

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Organophosphorus insecticide diazinon (DZN) is diffusely used in agriculture, home gardening, and crop peats. Much work so far has focused on the link between DZN exposure and the occurrence of neurological diseases, while little is known on the reproductive toxicological assessment on DZN exposure. This research aimed to investigate the underlying mechanisms of toxic hazards for DZN exposure on cultured porcine ovarian granulosa cells. We analyzed the oxidative stress, energy metabolism, DNA damage, apoptosis, and autophagy by using high-throughput RNA-seq, immunofluorescence, Western blotting, and real-time PCR. The combined data demonstrated that DZN exposure could cause excessive ROS and DNA damage, which induced apoptosis and autophagy by inhibiting the PI3K-AKT pathway. The down-regulated CYP19A1 protein and granulosa cell deaths increase the risk for developing premature ovarian failure and follicular atresia. In conclusion, DZN exposure has obvious reproductive toxicity by induction of granulosa cell death through pathways connected to DNA damage and oxidative stress by inhibiting the PI3K-AKT pathway.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Then, the 400 μL of binding buffer was incubated for 15 min at 25 °C in the dark box. Detection of fluorescence signals was under the fluorescence microscope (BX51, Olympus, Japan) …”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

Wang

Luo

et al. 2018

J. Agric. Food Chem.

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“…This anticoagulant protein may detect apoptotic cells exhibiting on their surface negatively charged phospholipids like phosphatidylserine. Several studies emphasized annexin V as a promising candidate biomarker for evaluating radiation toxicity in oncologic patients [108,109].…”

Section: Apoptosis Biomarkersmentioning

confidence: 99%

Molecular Biomarkers for Predicting Cancer Patient Radiosensitivity and Radiotoxicity in Clinical Practice

Gkikoudi,

Kalospyros,

Triantopoulou

et al. 2023

Applied Sciences

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Radiotherapy (RT) is a major part of cancer treatment. The reported variability in patient response to this modality can interfere with the continuation of best-possible care, promote side effects, and lead to long-term morbidity. Tools to predict a patient’s response to radiation could be highly useful in improving therapeutic outcomes while minimizing unnecessary and toxic exposure to radiation. This study investigates the potential of using molecular biomarkers as predictors of radiosensitivity in clinical practice. We review relative studies researching the positive correlation between various molecular biomarkers and patient radiosensitivity, including DNA damage response and repair proteins, inflammation and apoptosis markers, cell cycle regulators, and other biological markers. The clinical perspectives and applicability of these biomarkers in the prediction of radiosensitivity are also critically discussed. Conclusively, we underline the dynamics of molecular biomarkers to improve the efficacy and safety of radiotherapy in clinical practice and highlight the need for further research in this field. Identification of the most prominent markers is crucial for the personalization of therapies entailing ionizing radiation.

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“…The apoptotic process can be modulated by dietary chemopreventive substances [ 25 ]. Angiogenesis (the development of new blood vessels) is a complex vital process involving angiogenic substances generated by cancer cells and immune cells.…”

Section: Introductionmentioning

confidence: 99%

The potential preventive effect of dietary phytochemicals In Vivo

et al. 2023

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Introduction Chemoprevention refers to using specific substances during oncogenesis. Curcumin and catechins are both polyphenol types of phytochemicals present in curcuma longa and green tea. The effect of curcumin is synergistic with epigallocatechin gallate, the most abundant polyphenol in tea. Aim To evaluate and compares the chemopreventive effect of both green tea and curcumin (each individually and in combination) through induction of hamster buccal pouch carcinoma. Materials and methods Squamous cell carcinoma was chemically induced in fifty Syrian golden hamsters divided into 5 groups (10 each). The first group was used as a normal control group. The second group received the carcinogenic agent only. The other three groups received green tea, curcumin, and a combination of both, respectively. Flow cytometry, immunofluorescence, and immunohistochemical assays were used to evaluate apoptosis, proliferation, and angiogenesis. ANOVA test was used to analyze the results between the study groups. Results The cells of the positive control group (B) resulted in 11.57% apoptosis. In the study groups, treatment of the cells with green tea (C), and curcumin (D) and both of them (E) showed increased apoptosis. The fluorescent image in group B showed an increase of the red fluorescence in the nucleus and cytoplasm of the squamous cell carcinoma cells while groups C, D, and E showed a decrease of the red fluorescence in the nuclei of the squamous cell carcinoma cells. The microvessel density was higher in the positive control group as compared to the treated groups. Conclusions The combination of green tea and curcumin has a significant chemopreventive effect against oral carcinogenesis.

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Annexin V FITC conjugated as a radiation toxicity indicator in lymphocytes following radiation overexposure in radiotherapy programs

Cited by 7 publications

References 15 publications

Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

Molecular Biomarkers for Predicting Cancer Patient Radiosensitivity and Radiotoxicity in Clinical Practice

The potential preventive effect of dietary phytochemicals In Vivo

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