‘Annexins’ themed section

Flower, Rod; Perretti, Mauro

doi:10.1111/bph.13089

Cited by 4 publications

(4 citation statements)

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“…ANXA2 is a member of the annexin family, which contains 14 proteins in humans. Annexin proteins are involved in endocytosis, exocytosis, membrane trafficking and remodeling, and cytoskeleton interactions ( 18 , 19 ). Annexins interact with anionic phospholipids in a Ca 2+ -dependent manner ( 20 ), and different annexin proteins bind to distinct phospholipids in different intracellular environments ( 21 – 23 ).…”

Section: Introductionmentioning

confidence: 99%

“…Annexins interact with anionic phospholipids in a Ca 2+ -dependent manner ( 20 ), and different annexin proteins bind to distinct phospholipids in different intracellular environments ( 21 – 23 ). ANXA2 mainly binds phosphatidylethanolamine (PE), phosphatidylinositol bisphosphate PI (4,5) P2, and cholesterol ( 19 ). ANXA2 is both membrane-associated and cytosolic ( 24 ).…”

Section: Introductionmentioning

confidence: 99%

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Annexin A2 facilitates endocytic trafficking of antisense oligonucleotides

et al. 2016

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Chemically modified antisense oligonucleotides (ASOs) designed to mediate site-specific cleavage of RNA by RNase H1 are used as research tools and as therapeutics. ASOs modified with phosphorothioate (PS) linkages enter cells via endocytotic pathways. The mechanisms by which PS-ASOs are released from membrane-enclosed endocytotic organelles to reach target RNAs remain largely unknown. We recently found that annexin A2 (ANXA2) co-localizes with PS-ASOs in late endosomes (LEs) and enhances ASO activity. Here, we show that co-localization of ANXA2 with PS-ASO is not dependent on their direct interactions or mediated by ANXA2 partner protein S100A10. Instead, ANXA2 accompanies the transport of PS-ASOs to LEs, as ANXA2/PS-ASO co-localization was observed inside LEs. Although ANXA2 appears not to affect levels of PS-ASO internalization, ANXA2 reduction caused significant accumulation of ASOs in early endosomes (EEs) and reduced localization in LEs and decreased PS-ASO activity. Importantly, the kinetics of PS-ASO activity upon free uptake show that target mRNA reduction occurs at least 4 hrs after PS-ASOs exit from EEs and is coincident with release from LEs. Taken together, our results indicate that ANXA2 facilitates PS-ASO trafficking from early to late endosomes where it may also contribute to PS-ASO release.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Annexin A2 facilitates endocytic trafficking of antisense oligonucleotides

et al. 2016

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“…Annexin is highly conserved and ubiquitously distributed in various body cell types and accounts for about 0.5–2% of the total cell protein [ 1 , 2 ]. It is involved in membrane transport and a series of calmodulin-dependent activities on the surface of the cell membrane, including vesicular transport, membrane fusion in exocytosis, signal transduction, and formation of calcium channels, and also regulates inflammatory response, cell differentiation, and interaction between cytoskeletal proteins [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Annexin A3 as a Prognostic Biomarker for Breast Cancer: A Retrospective Study

Zhou

Yong

Yang

et al. 2017

BioMed Research International

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To validate the correlation between ANXA3 expression and prognosis in breast cancer, a retrospective study encompassing 309 breast cancer patients was performed. The expression of ANXA3 was determined by the immunohistochemical examination of tissue sections by the Max Vision™ method. The ANXA3 levels in the patient samples were validated for the prognosis based on age, menopause status, tumor size, tumor node, metastasis stage, the number of lymphatic metastases, oncology grade, and molecular subtyping. An elevated expression of ANXA3 was detected in breast cancer samples, compared to adjacent tissue samples, and significant correlation depending on the number of lymphatic metastases (P = 0.001) and histological grade (P = 0.004) was observed. The number of lymphatic metastases and ANXA3 expression were identified as independent risk factors affecting the disease-free survival and overall survival. Significantly (P < 0.002) higher level of ANXA3 was detected in triple-negative breast cancer compared to other subtypes. There was no significant (P > 0.05) change in the expression of ANXA3 with respect to age, menopausal status, tumor size, and clinical stage. The findings implicate the expression of ANXA3 with the natural progression of breast cancer and associate it with increased lymphatic metastasis. The study validates the use of ANXA3 as a potential prognosis biomarker for breast cancer.

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“…Annexins are a conserved family of Ca 2+ and phospholipid binding proteins that participate in membrane transport and a range of calcium‐regulated activities on the cell membrane surface. They are ubiquitously distributed in various body cell types and account for about 0.5–2% of the total cell protein (Flower & Perretti, ; Schlaepfer & Haigler, ). Despite numerous studies having been conducted on annexins, the function of annexin3 (ANX3) is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

The potential role of Annexin 3 in diapause embryo restart of Artemia sinica and in response to stress of low temperature

Yao

Huang

et al. 2019

Molecular Reproduction Devel

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Annexins are highly conserved and ubiquitous in various somatic cell types. They are involved in membrane transport and a range of calcium-regulated activities on the cell membrane surface, including vesicular transport, membrane fusion in exocytosis, signal transduction, and formation of calcium channels. They also regulate inflammatory response, cell differentiation, and interaction between cytoskeletal proteins. In this study, for the first time, an ANX3 gene from Artemia sinica (As-anx3) was cloned. The As-anx3 full-length complementary DNA comprises 1,024 bp and has a 948 bp open reading frame encoding a 315-amino-acid polypeptide with four ANX domains. The profiles of both As-ANX3 mRNA and protein expression exhibited peaks at the 0 hr stage and had the same significant downregulation trend throughout the post-diapause embryo development stage. The ERK1/2, the phosphorylation levels of ERK1/2, and cell cycle-related protein (CDK4) expressions were analyzed by western blot analysis. The results showed that CDK4 presented a significantly ascending trend from 0 and 40 hr, although the phosphorylation levels of ERK1/2 did not increase significantly. The transcriptional and protein expressions of As-ANX3 were highly upregulated when the temperature was lowered from 25 to 15°C, but the expressions showed a gradual downward trend when the temperature was further lowered to 5°C. These results indicated that As-ANX3 plays a crucial role in restarting diapause and low-temperature stress in A. sinica. K E Y W O R D Sannexin 3, Artemia sinica, diapause embryo development

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‘Annexins’ themed section

Abstract: Linked Articles This article is part of a themed section on Annexins VII Programme. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2015.172.issue-7

Cited by 4 publications

References 10 publications

Annexin A2 facilitates endocytic trafficking of antisense oligonucleotides

Annexin A2 facilitates endocytic trafficking of antisense oligonucleotides

Annexin A3 as a Prognostic Biomarker for Breast Cancer: A Retrospective Study

The potential role of Annexin 3 in diapause embryo restart of Artemia sinica and in response to stress of low temperature

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