Annotation and overview of the <i>Pseudomonas savastanoi</i> pv. savastanoi NCPPB 3335 draft genome reveals the virulence gene complement of a tumour‐inducing pathogen of woody hosts

Rodrı́guez-Palenzuela, Pablo; Matas, Isabel; Murillo, Jesús; López‐Solanilla, Emilia; Bardaji, Leire; Pérez‐Martínez, Isabel; Rodríguez-Moskera, Martín E.; Penyalver, Ramón; López, Marı́a M.; Quesada, José Miguel; Biehl, Bryan S.; Perna, Nicole T.; Glasner, Jeremy D.; Cabot, Eric L.; Neeno-Eckwall, Eric C.; Ramos, Cayo

doi:10.1111/j.1462-2920.2010.02207.x

Cited by 83 publications

(111 citation statements)

References 67 publications

(99 reference statements)

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“…tomato DC3000 and P. savastanoi pv. savastanoi NCPPB3335, and it was highly conserved among P. syringae bacteria belonging to genome species 3 [64,68]. In the promoter region upstream of the matE gene of Psn23, an hrp box sequence was found, suggesting its HrpL-dependent transcription.…”

Section: Discussionmentioning

confidence: 92%

Indole-3-acetic acid in plant–pathogen interactions: a key molecule for in planta bacterial virulence and fitness

Cerboneschi

Decorosi

Biancalani

et al. 2016

Research in Microbiology

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The plant pathogenic bacterium Pseudomonas savastanoi, the causal agent of olive and oleander knot disease, uses the so-called "indole-3-acetamide pathway" to convert tryptophan to indole-3-acetic acid (IAA) via a two-step pathway catalyzed by enzymes encoded by the genes in the iaaM/iaaH operon. Moreover, pathovar nerii of P. savastanoi is able to conjugate IAA to lysine to generate the less biologically active compound IAA-Lys via the enzyme IAA-lysine synthase encoded by the iaaL gene. Interestingly, iaaL is now known to be widespread in many Pseudomonas syringae pathovars, even in the absence of the iaaM and iaaH genes for IAA biosynthesis.Here, two knockout mutants, DiaaL and DiaaM, of strain Psn23 of P. savastanoi pv. nerii were produced. Pathogenicity tests using the host plant Nerium oleander showed that DiaaL and DiaaM were hypervirulent and hypovirulent, respectively and these features appeared to be related to their differential production of free IAA. Using the Phenotype Microarray approach, the chemical sensitivity of these mutants was shown to be comparable to that of wild-type Psn23. The main exception was 8 hydroxyquinoline, a toxic compound that is naturally present in plant exudates and is used as a biocide, which severely impaired the growth of DiaaL and DiaaM, as well as growth of the non-pathogenic mutant DhrpA, which lacks a functional Type Three Secretion System (TTSS). According to bioinformatics analysis of the Psn23 genome, a gene encoding a putative Multidrug and Toxic compound Extrusion (MATE) transporter, was found upstream of iaaL. Similarly to iaaL and iaaM, its expression appeared to be TTSS-dependent. Moreover, auxin-responsive elements were identified for the first time in the modular promoters of both the iaaL gene and the iaaM/iaaH operon of P. savastanoi, suggesting their IAA-inducible transcription. Gene expression analysis of several genes related to TTSS, IAA metabolism and drug resistance confirmed the presence of a concerted regulatory network in this phytopathogen among virulence, fitness and drug efflux.

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Section: Discussionmentioning

confidence: 92%

Indole-3-acetic acid in plant–pathogen interactions: a key molecule for in planta bacterial virulence and fitness

Cerboneschi

Decorosi

Biancalani

et al. 2016

Research in Microbiology

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“…savastanoi (Marchi et al, 2009;Matas et al, 2009;Pérez-Martínez et al, 2008Ramos et al, 2012;Rodríguez-Moreno et al, 2009;Scortichini et al, 2004;Surico et al, 1985). The description of several virulence factors Iacobellis et al, 1994;Matas et al, 2009;Matas et al, 2012;Pérez-Martínez et al, 2010;Ramos et al, 2012;Rodríguez-Moreno et al, 2008Rodríguez-Palenzuela et al, 2010;Rojas et al, 2004;Surico et al, 1985) and the normal localization on the intercellular space of the parenchymal tissue as a biofilm structure are, to our knowledge, the main recent discoveries (Rodríguez-Moreno et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The Pathological input file was prepared according to the instructions in the Pathway Tools (version 16.5) user's guide by feeding the software with the latest annotated files of Pseudomonas savastanoi pv. savastanoi and E. toletana (Passos da Silva et al, 2013;Rodríguez-Palenzuela et al, 2010) draft genomes, and merged files of the Pseudomonas savastanoi pv. savastanoi/E.…”

Section: Methodsmentioning

confidence: 99%

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Bacterial multispecies studies and microbiome analysis of a plant disease

et al. 2014

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Although the great majority of bacteria found in nature live in multispecies communities, microbiological studies have focused historically on single species or competition and antagonism experiments between different species. Future directions need to focus much more on microbial communities in order to better understand what is happening in the wild. We are using olive knot disease as a model to study the role and interaction of multispecies bacterial communities in disease establishment/development. In the olive knot, non-pathogenic bacterial species (e.g. Erwinia toletana) co-exist with the pathogen (Pseudomonas savastanoi pv. savastanoi); we have demonstrated cooperation among these two species via quorum sensing (QS) signal sharing. The outcome of this interaction is a more aggressive disease when co-inoculations are made compared with single inoculations. In planta experiments show that these two species co-localize in the olive knot, and this close proximity most probably facilitates exchange of QS signals and metabolites. In silico recreation of their metabolic pathways showed that they could have complementing pathways also implicating sharing of metabolites. Our microbiome studies of nine olive knot samples have shown that the olive knot community possesses great bacterial diversity; however. the presence of five genera (i.e. Pseudomonas, Pantoea, Curtobacterium, Pectobacterium and Erwinia) can be found in almost all samples.

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“…It is formed by a micropropagated olive plant, coming from an in vitro germinated seed and a Psv strain (NCPPB 3335) producing the characteristic symptoms of olive knot disease in both woody and micropropagated olive plants (Pérez-Martínez et al, 2007;Rodríguez-Moreno et al, 2008). Besides, Psv strain NCPPB 3335 has been studied in depth and many genetic resources are available because its genome has been sequenced and analyzed using appropriate bioinformatic tools (Rodríguez-Palenzuela et al, 2010;Matas, 2010).…”

Section: Endophytic Populations Of Psvmentioning

confidence: 99%

Epidemiology and Control of Plant Diseases Caused by Phytopathogenic Bacteria: The Case of Olive Knot Disease Caused by Pseudomonas savastanoi pv. savastanoi

Quesada¹,

Penyalver²,

López³

2012

Plant Pathology

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Annotation and overview of the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 draft genome reveals the virulence gene complement of a tumour‐inducing pathogen of woody hosts

Cited by 83 publications

References 67 publications

Indole-3-acetic acid in plant–pathogen interactions: a key molecule for in planta bacterial virulence and fitness

Indole-3-acetic acid in plant–pathogen interactions: a key molecule for in planta bacterial virulence and fitness

Bacterial multispecies studies and microbiome analysis of a plant disease

Epidemiology and Control of Plant Diseases Caused by Phytopathogenic Bacteria: The Case of Olive Knot Disease Caused by Pseudomonas savastanoi pv. savastanoi

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