Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow

Seyer, Alexandre; Boudah, Samia; Broudin, Simon; Junot, Christophe; Colsch, Benoît

doi:10.1007/s11306-016-1023-8

Cited by 42 publications

(44 citation statements)

References 59 publications

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“…Ceramide lipid species were enriched in the lower phase (organic phase) and analysed using an untargeted lipidomic approach by liquid chromatography coupled with high-resolution MS (LC-HRMS) as described [20]. MS data treatments were performed using the XCMS software (https://metlin.scripps.edu/).…”

Section: Methodsmentioning

confidence: 99%

Lipoprotein lipase in hypothalamus is a key regulator of body weight gain and glucose homeostasis in mice

et al. 2017

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Aims/hypothesis Regulation of energy balance involves the participation of many factors, including nutrients, among which are circulating lipids, acting as peripheral signals informing the central nervous system of the energy status of the organism. It has been shown that neuronal lipoprotein lipase (LPL) participates in the control of energy balance by hydrolysing lipid particles enriched in triacylglycerols. Here, we tested the hypothesis that LPL in the mediobasal hypothalamus (MBH), a well-known nucleus implicated in the regulation of metabolic homeostasis, could also contribute to the regulation of body weight and glucose homeostasis. MethodsWe injected an adeno-associated virus (AAV) expressing Cre-green fluorescent protein into the MBH of Lpl-floxed mice (and wild-type mice) to specifically decrease LPL activity in the MBH. In parallel, we injected an AAV overexpressing Lpl into the MBH of wild-type mice. We then studied energy homeostasis and hypothalamic ceramide content.Results The partial deletion of Lpl in the MBH in mice led to an increase in body weight compared with controls (37.72 ± 0.7 g vs 28.46 ± 0.12, p < 0.001) associated with a decrease in locomotor activity. These mice developed hyperinsulinaemia and glucose intolerance. This phenotype Elise Laperrousaz and Valentine S. Moullé contributed equally to this work.Yongping Wang, who took part in this research, died before this work was published.Electronic supplementary material The online version of this article (doi:10.1007/s00125-017-4282-7) contains peer-reviewed but unedited supplementary material, which is available to authorised users. * Christophe MagnanChristophe.magnan@univ-paris-diderot.fr also displayed reduced expression of Cers1 in the hypothalamus as well as decreased concentration of several C18 species of ceramides and a 3-fold decrease in total ceramide intensity. Conversely, overexpression of Lpl specifically in the MBH induced a decrease in body weight.Conclusions/interpretation Our study shows that LPL in the MBH is an important regulator of body weight and glucose homeostasis.

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Section: Methodsmentioning

confidence: 99%

Lipoprotein lipase in hypothalamus is a key regulator of body weight gain and glucose homeostasis in mice

et al. 2017

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“…The samples were separated on an HTC PAL system (CTC Analytics AG) coupled with a Transcend 1250 liquid chromatographic system (Thermo Fisher Scientific, Inc.) using a Kinetex C8 2.6 μm 2.1 × 150 mm column (Phenomenex, Sydney, NSW, Australia). High-resolution mass spectrometry using a Q-Exactive mass spectrometer (Thermo Fisher Scientific, Inc.) was performed as previously described (66). The relative amount of each lipid was semi-quantified as the area of its corresponding chromatographic peak.…”

Section: Lipidomic Analysismentioning

confidence: 99%

High‐dose biotin restores redox balance, energy and lipid homeostasis, and axonal health in a model of adrenoleukodystrophy

et al. 2020

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Biotin is an essential cofactor for carboxylases that regulates the energy metabolism. Recently, high‐dose pharmaceutical‐grade biotin (MD1003) was shown to improve clinical parameters in a subset of patients with chronic progressive multiple sclerosis. To gain insight into the mechanisms of action, we investigated the efficacy of high‐dose biotin in a genetic model of chronic axonopathy caused by oxidative damage and bioenergetic failure, the Abcd1− mouse model of adrenomyeloneuropathy. High‐dose biotin restored redox homeostasis driven by NRF‐2, mitochondria biogenesis and ATP levels, and reversed axonal demise and locomotor impairment. Moreover, we uncovered a concerted dysregulation of the transcriptional program for lipid synthesis and degradation in the spinal cord likely driven by aberrant SREBP‐1c/mTORC1signaling. This resulted in increased triglyceride levels and lipid droplets in motor neurons. High‐dose biotin normalized the hyperactivation of mTORC1, thus restoring lipid homeostasis. These results shed light into the mechanism of action of high‐dose biotin of relevance for neurodegenerative and metabolic disorders.

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“…Afterwards, the coupling of liquid chromatography to a high‐resolutive mass spectrometer, such as an Orbitrap interfaced to a quadrupole mass filter, allowed the separation of isobar species, as previously developed by Ref. to describe the human cerebrospinal fluid lipidome. The same methodology was used, whose annotated 462 molecular species belonging to 13 classes of lipids (Ceramide, SM, lysophosphatidylcholine, PC, PE, DG, TAG, phosphatidic acid, phosphatidylglycerol, PI, PS, lysophosphatidyl ethanolamine, and FA), of which 300 belonged to TAG and 66 of which were identified for the first time in caprine and bovine milks.…”

Section: New Approaches For Determining Major and Minor Components Ofmentioning

confidence: 99%

Milk Fat Globule in Ruminant: Major and Minor Compounds, Nutritional Regulation and Differences Among Species

Bernard

Bonnet

Delavaud

et al. 2018

Euro J Lipid Sci & Tech

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Recent knowledge is presented on the composition of ruminant milk fat fractions and the nutritional strategies known to alter their amount. The development of lipidomic and proteomic analyses has allowed for the characterization of minor components, such as proteins, liposoluble vitamins, and phospholipids of the milk fat globule (MFG), in addition to the triacylglycerols (TAG), which are the major constituents of the MFG core. Few differences in these components among ruminant species exist, and they have been outlined mainly on the fatty acids (FA) profile of the TAG, whereas comparative data are still lacking on vitamins and proteins. The effects of dietary treatments enriched in n‐3 polyunsaturated FA (PUFA) on the composition of the milk fat fraction are explored. In particular, pasture and plant oilseeds increase milk n‐3 PUFA and cis‐9,trans‐11 CLA and decrease saturated FA, whereas data with new feed resources, such as algae, are still rare. The peculiarities of the response of the milk fat to diets that induce a milk fat depression in cows but in lesser extent in small ruminants are described. The potential effects of polar lipids, proteins, and liposoluble vitamins of the MFG on human health are reviewed, highlighting the nutraceutical properties of milk. Practical Applications: This review provides an overview of the different components of the milk fat fraction in ruminant species and on nutritional strategies to alter their amounts to improve the nutritional quality of milk. Furthermore, this review presents recent data on species peculiarities of the milk fat fraction composition and of its response to nutritional factors, which offers a promising model to identify news levers of regulation of this fraction and foster the identification of new feeding strategies to better control milk fat composition and feed efficiency. This review synthesizes data on the composition of the milk fat fraction in ruminant species, reports advances in nutritional strategies to alter their amounts and species‐specific responses to nutrition, as well as the potential effects on human health of the major and minor components of this fraction.

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Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow

Cited by 42 publications

References 59 publications

Lipoprotein lipase in hypothalamus is a key regulator of body weight gain and glucose homeostasis in mice

Lipoprotein lipase in hypothalamus is a key regulator of body weight gain and glucose homeostasis in mice

High‐dose biotin restores redox balance, energy and lipid homeostasis, and axonal health in a model of adrenoleukodystrophy

Milk Fat Globule in Ruminant: Major and Minor Compounds, Nutritional Regulation and Differences Among Species

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