Annotation of the human serum metabolome by coupling three liquid chromatography methods to high-resolution mass spectrometry

Parallel Metabolomic Profiling of Cerebrospinal Fluid and Serum for Identifying Biomarkers of Injury Severity after Acute Human Spinal Cord Injury

“…In total, 1213 and 2316 peak pairs or metabolites could be detected in CSF and serum, respectively, and 102 CSF and 108 serum metabolites could be positively identified. Compared to previous work on metabolome profiling that typically reports less than 100 amine- and phenol-containing metabolites1720, dansylation labeling LC-MS achieved a much higher coverage for this targeted sub-metabolome.…”

Major microbiota dysbiosis in severe obesity: fate after bariatric surgery

“…Chromatographic conditions were adapted to screen microbiota-derived metabolites as described 22. Data were curated, normalised and annotated, yielding 242 different metabolites.…”

Non-targeted screening approaches for contaminants and adulterants in food using liquid chromatography hyphenated to high resolution mass spectrometry

“…A (2015), http://dx.doi.org/10.1016/j.chroma.2015.08.059 only ionize in positive or negative mode. This is illustrated by the data shown collected by Boudah et al in Table 1 [43]. For example, 130 metabolites were detected using negative mode with a HILIC (hydrophilic interaction chromatography) column, where 103 compounds can be identified without the need of another chromatographic method.…”

“…Separation strategies for untargeted metabolomics were reviewed previously, and include suggestions for potential column chemistries and ion pairing agents that can be implemented [42]. By using three different column chemistries (reverse phase, HILIC, and PFPP), Boudah et al determined that less than 12% of the detected entities were detected in all three separations [43], which emphasizes the advantage of analyzing samples under different conditions. This is also illustrated by the data shown in Table 1.…”

“…Abbreviations: hydrophilic interaction chromatography (HILIC), pentafluorophenylpropyl (PFPP). Adapted from [43] ESI-MS/MS detection [45]. These approaches serve to automatically reduce sample complexity and aid in enhanced detection of different compound chemistries.…”