Annual bluegrass (Poa annua L.) is a problematic weed on golf courses, sports turf, sod production fields, and irrigated lawns, but few options exist for control of annual bluegrass in Kentucky bluegrass (Poa pratensis L.). However, mesotrione has shown promising activity against annual bluegrass and adequate turf safety on Kentucky bluegrass. Experiments were completed from 2004 through 2010 in Urbana, IL, and/or West Lafayette, IN, to refine application strategies for annual bluegrass control with mesotrione in the fall. Though acceptable control (> 80%) was obtained in some experiments, control was inconsistent or marginal depending on the location and year. Our current recommendations for fall‐applied mesotrione would include three applications, starting in mid‐ to late September, at rates between 0.11 and 0.17 kg ai/ha per application, and without follow‐up applications in April. Further research will have to improve consistency of Poa annua control with mesotrione for widespread commercial use.