, J. T. 2009. The green manure value of seven clover species grown as annual crops on low and high fertility temperate soils. Can. J. Plant Sci. 89: 465Á476. Annual and perennial clover species may differ in green manure value. Seven clover (Trifolium) species were grown as annual crops on low fertility (Breton) and high fertility (Edmonton) soils in Alberta. Four annual clovers [balansa (T. michelianum Savi), berseem (T. alexandrinum L.), crimson (T. incarnatum L.), and Persian (T. resupinatum L.)], three perennial clovers [alsike (T. hybridum L.), red (T. pratense L.), and white Dutch (T. repens L.)] and a non-legume reference crop [fall rye (Secale cereale L.)] were ploughed-down as green manure in autumn, and followed by barley (Hordeum vulgare L.). Annual clovers had greater biomass yields than perennial clovers, and berseem clover had the highest yield. At Breton, mean biomass N content was greater for perennial clovers (2.9 g N kg(1 ) than annual clovers (1.9 g N kg(1 ). Clover biomass at Breton yielded an average of 77 kg N ha (1 , with N derived from the atmosphere averaging 88% by N difference method and 75% by 15 N natural abundance method. At Edmonton, the green manures had few effects on soil nitrate and subsequent barley yields. At Breton, all clover green manures except balansa increased barley yields, and grain yields were greater following perennial clovers than annual clovers in one year. Annual clovers will provide forage biomass and add N in areas where rainfall is adequate, and they may be preferable under zero tillage. However there is no advantage of annual clovers, relative to perennial clovers, in terms of N supply. . Les espe`ces de tre`fle annuelles ont donne´plus de biomasse que les espe`ces vivaces, le tre`fle d'Alexandrie enregistrant le meilleur rendement. À Breton, la teneur en N moyenne de la biomasse e´tait plus e´leve´e pour les espe`ces vivaces (2,9 g de N par kg) que pour les espe`ces annuelles (1,9 g de N par kg). Toujours aB reton, la biomasse du tre`fle a donne´en moyenne 77 kg de N par hectare, l'azote issu de l'atmosphe`re en repre´sentant en moyenne 88 % quand on calcule sa proportion par soustraction et 75 % quand on la calcule en fonction de l'abondance de l'isotope 15 N dans la nature. À Edmonton, les engrais verts ont eu peu d'incidence sur les nitrates du sol et le rendement subse´quent de l'orge. À Breton, tous les engrais verts a`base de tre`fle sauf le tre`fle de Micheli ont accru le rendement de l'orge et le rendement ce´re´alier e´tait plus important apre`s la culture du tre`fle vivace qu'apre`s celle du tre`fle annuel. Les espe`ces annuelles de tre`fle produisent de la biomasse fourrage`re et ajoutent de l'azote au sol des endroits ou`il pleut suffisamment; il pourrait eˆtre pre´fe´rable de les cultiver quand on pratique le non-travail du sol. Ne´anmoins, cultiver des espe`ces annuelles plutoˆt que des espe`ces vivaces de tre`fle ne pre´sente aucun avantage en ce qui concerne l'apport de N.