Anoctamin 1 Positive Esophageal Interstitial Cajal Cells in Late Stage Human Embryos

Rusu, Mugurel Constantin; Poalelungi, Cristian; Vrapciu, Alexandra Diana; Păduraru, Luminiţa; Didilescu, Andreea Cristiana; Stan, Cristinel Ionel

doi:10.1002/ar.22837

Cited by 6 publications

(3 citation statements)

References 50 publications

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“…There are studies proving CD34 and/or CD31 positive phenotypes for ETCs [ 4 , 5 , 20 ]. Moreover, CD105 (endoglin) is up-regulated in activated endothelial cells thus being an appropiate marker for identifying endothelial sprouts [ 21 ] and ETCs.…”

Section: Discussionmentioning

confidence: 99%

Endocardial Tip Cells in the Human Embryo – Facts and Hypotheses

et al. 2015

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Experimental studies regarding coronary embryogenesis suggest that the endocardium is a source of endothelial cells for the myocardial networks. As this was not previously documented in human embryos, we aimed to study whether or not endothelial tip cells could be correlated with endocardial-dependent mechanisms of sprouting angiogenesis. Six human embryos (43–56 days) were obtained and processed in accordance with ethical regulations; immunohistochemistry was performed for CD105 (endoglin), CD31, CD34, α-smooth muscle actin, desmin and vimentin antibodies. Primitive main vessels were found deriving from both the sinus venosus and aorta, and were sought to be the primordia of the venous and arterial ends of cardiac microcirculation. Subepicardial vessels were found branching into the outer ventricular myocardium, with a pattern of recruiting α-SMA+/desmin+ vascular smooth muscle cells and pericytes. Endothelial sprouts were guided by CD31+/CD34+/CD105+/vimentin+ endothelial tip cells. Within the inner myocardium, we found endothelial networks rooted from endocardium, guided by filopodia-projecting CD31+/CD34+/CD105+/ vimentin+ endocardial tip cells. The myocardial microcirculatory bed in the atria was mostly originated from endocardium, as well. Nevertheless, endocardial tip cells were also found in cardiac cushions, but they were not related to cushion endothelial networks. A general anatomical pattern of cardiac microvascular embryogenesis was thus hypothesized; the arterial and venous ends being linked, respectively, to the aorta and sinus venosus. Further elongation of the vessels may be related to the epicardium and subepicardial stroma and the intramyocardial network, depending on either endothelial and endocardial filopodia-guided tip cells in ventricles, or mostly on endocardium, in atria.

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Section: Discussionmentioning

confidence: 99%

Endocardial Tip Cells in the Human Embryo – Facts and Hypotheses

et al. 2015

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“…A particular situation is that of minor patients, where prosthesis is recommended to be done after the written consent of the parents and after a prior clinical examination to exclude congenital malformations of the oral cavity [24][25][26][27][28][29][30][31].…”

Section: Resultsmentioning

confidence: 99%

The Use of Diacrylic Resins in Dental Restorations

Romanec¹,

Stan²,

Hînganu³

et al. 2018

Rev. Chim.

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The materials as well as the procedures used successfully in modern dental restorations are based on scientific selection criteria, which can predict outstanding results, both in terms of physiological aspects and the durability of the performed maneuvers.The rationale of using certain materials, as the success or failure in using certain techniques, has contributed to the evolution of dental restorations. The optimal way to combine the material with its applicability and finishing technique implies an exhaustive approach to the subject that still requires research, that adress to specialists, in order to improve its refinement and development.Among the restoration materials, those that satisfy the aesthetic requirements of the patients are considered to be composite resins. With all the advantages resulted from their intrinsic characteristics, it should be noted that composite resins still have inconveniences, primarily linked to the polymerization shrinkage, which causes a gap between the obturation and the dental tissue, resulting in the formationof marginal microinfiltrations with all the drawbacks associated with it. The purpose of the experimental research is to evaluate the marginal microinfiltrations at the level of the class II cavities restoration with composite diacrylic resins, according to various techniques of restoration and to establish the optimal technique to minimize side effects.

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“…Nestin is a type VI intermediate filament protein which is expressed in progenitor and mitotically active stem cells, such as neuroepithelial cells, neural crest‐derived cells, neural stem cells, retinal cells, as well as in newly formed microvascular endothelia and bone marrow stromal cells (Calenic et al, ; Enache et al, ; Rusu et al, ; Vrapciu et al, ). Primitive mesenchymal cells, including nestin‐expressing cells (NECs) and CXCL12‐abundant reticular (CAR) cells, act as niche elements for hematopoietic stem cells (HSCs) in the bone marrow (BM) (Sugiyama and Nagasawa, ).…”

mentioning

confidence: 99%

Regenerative Potential of Human Schneiderian Membrane: Progenitor Cells and Epithelial‐Mesenchymal Transition

Derjac-Arama

Sarafoleanu

Manea

et al. 2015

The Anatomical Record

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An innate osteogenic potential of the Schneiderian membrane (SM) is progressively assessed in studies ranging from non-human species to human subjects. It has relevance for endosteal placement and osseointegration. Nestin-expressing osteogenic progenitor cells are allegedly involved in bone formation and remodelling. Nestin phenotype was not assessed previously in human SM. We therefore aimed to fill that particular gap in the literature. Bioptic samples of human adult SM were obtained during surgery from eight adult patients, operated for nonmalignant pathologies. Immunohistochemistry on paraffin-embedded tissue samples used primary antibodies against nestin, CD45, CD146, cytokeratin 7 (CK7), and alpha-smooth muscle actin (a-SMA). Nestin expression was consistently found in endothelial cells, and was scarcely encountered in pericytes, putative stromal stem/progenitor cells, as well as in glandular epithelial cells. Moreover, woven bone formation in the periosteal layer of the SM can also be regarded as evidence of the osteogenic potential of this membrane. Nestin and CD45 expression in cells of the primary bone supports the osteogenic potential of SM nestinexpressing cells and a possible involvement of hematopoietic stem cells in maxillary sinus floor remodeling. CD146, a known inducer of epithelial-mesenchymal transition (EMT), was expressed in epithelia, as was CK7. Isolated stromal cells were found expressing CD146, CK7 and a-SMA, suggesting that regenerative processes happening in the SM may also involve processes of EMT which generate stem/progenitor cells.

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Anoctamin 1 Positive Esophageal Interstitial Cajal Cells in Late Stage Human Embryos

Cited by 6 publications

References 50 publications

Endocardial Tip Cells in the Human Embryo – Facts and Hypotheses

Endocardial Tip Cells in the Human Embryo – Facts and Hypotheses

The Use of Diacrylic Resins in Dental Restorations

Regenerative Potential of Human Schneiderian Membrane: Progenitor Cells and Epithelial‐Mesenchymal Transition

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