Poor osseointegration and infection
are among the major challenges
of 316L stainless steel (SS) implants in orthopedic applications.
Surface modifications to obtain a nanostructured topography seem to
be a promising method to enhance cellular interactions of 316L SS
implants. In this study, arrays of nanodimples (NDs) having controlled
feature sizes between 25 and 250 nm were obtained on 316L SS surfaces
by anodic oxidation (anodization). Results demonstrated that the fabrication
of NDs increased the surface area and, at the same time, altered the
surface chemistry of 316L SS to provide chromium oxide- and hydroxide-rich
surface oxide layers. In vitro experiments showed that ND surfaces
promoted up to a 68% higher osteoblast viability on the fifth day
of culture. Immunofluorescence images confirmed a well-spread cytoskeleton
organization on the ND surfaces. In addition, higher alkaline phosphate
activity and calcium mineral synthesis were observed on the ND surfaces
compared to non-anodized 316L SS. Furthermore, a 71% reduction in Staphylococcus aureus (S. aureus) and a 58% reduction in Pseudomonas aeruginosa (P. aeruginosa) colonies were observed
on the ND surfaces having a 200 nm feature size compared to non-anodized
surfaces at 24 h of culture. Cumulatively, the results showed that
a ND surface topography fabricated on 316L SS via anodization upregulated
the osteoblast viability and functions while preventing S. aureus and P. aeruginosa biofilm synthesis.