Anomalies d’expression de la synaptophysine dans la maladie de Hirschsprung

Corsois, L.; Boman, Françoise; Sfeir, Rony; Besson, R.; Gottrand, Frédéric; Boccon‐Gibod, Liliane

doi:10.1016/s0242-6498(04)93996-x

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…Additional enzyme histochemistry staining with acetylcholinesterase (AChE) and NADH diaphorase has to be performed on fresh frozen specimens and is used in one hospital. Calretinin (positive in the control sample), S100 (positive in the control sample), synaptophysin (positive in the control sample) and AChE staining (negative in the control sample) highlight the nerve hypertrophy while NADH diaphorase and calretinin place the emphasis on the ganglion cells in the specimen [ 14 , 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

Rectal Biopsy for Hirschsprung’s Disease: A Multicentre Study Involving Biopsy Technique, Pathology and Complications

Vervloet,

De Backer,

Heyman

et al. 2023

Children

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Background: The heterogeneity of rectal biopsy techniques has encouraged us to search for a surgical and pathological standardisation of this diagnostic technique to exclude Hirschsprung’s disease. The different amounts of information on the anatomopathology report prompted us to compile a template for the anatomopathology report for diagnostic rectal biopsies for surgical colleagues and pathologists working on Hirschsprung’s disease. Methods: We gathered the anonymous biopsy information and its pathology information from five hospitals for all patients in which rectal biopsies were taken to diagnose Hirschsprung’s disease over two years (2020–2021). Results: Of the 82 biopsies, 20 suction (24.4%), 31 punch (37.8%) and 31 open biopsies (37.8%) were taken. Of all biopsies, 69 were conclusive (84.2%), 13 were not (15.8%). In the suction biopsy group, 60% were conclusive and 40% were not; for punch biopsy, the values were 87% and 13%, respectively and for open biopsy, 97% and 3%. Inconclusive results were due to insufficient submucosa in 6/8 suction biopsies, 4/4 punch biopsies and 0/1 open biopsies. An insufficient amount of submucosa was the reason for an inconclusive result in 6/20 cases (30%) after suction biopsy, 4/31 (12.9%) cases after punch biopsy and 0 cases (0%) after open biopsy. We had one case with major postoperative bleeding post suction biopsy; there were no further adverse effects after biopsy. Conclusions: Diagnostic rectal biopsies in children are safe. Non-surgical biopsies are more likely to give inconclusive results due to smaller amounts of submucosa present in the specimen. Open biopsies are especially useful when previous non-surgical biopsies are inconclusive. An experienced pathologist is a key factor for the result. The anatomopathology report should specify the different layers present in the specimen, the presence of ganglion cells and hypertrophic nerve fibres, their description and a conclusion.

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Section: Methodsmentioning

confidence: 99%

Rectal Biopsy for Hirschsprung’s Disease: A Multicentre Study Involving Biopsy Technique, Pathology and Complications

Vervloet,

De Backer,

Heyman

et al. 2023

Children

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“…The nervous plexus contains mature neuroganglion cells which maintain intestinal peristalsis to transport food (2). Without neuroganglion cells, preganglionic fibers of the parasympathetic ganglion from sacral spinal cord segments S2–S4 are not able to form synapses and generate postganglionic fibers in the intestinal tract, which causes preganglionic nerve fiber hyperplasia and incoordination of intestinal systaltic and peristaltic movement, resulting in difficulties in food transportation (3). …”

Section: Introductionmentioning

confidence: 99%

Differences in the development of autogenous nerves between the upper and lower urinary tract

Chen

Cai

Shi

et al. 2013

Experimental and Therapeutic Medicine

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The aim of this study was to observe the development and distribution of autogenous nerves in the urinary tract of New Zealand rabbits. Animals of various ages were used in this study, including 2, 3 and 3.5 weeks (gestational ages) and 1, 4, 8 and 12 weeks (postnatal). Samples were selected at various sites of the urinary tract. Immunohistochemical staining methods were used to investigate the nervous plexus and neuroganglia on the wall of the urinary tract. Myenteric plexuses and ganglia first appeared at the gestational age of 3 weeks. They decreased in the pelvis and ureter as the animals developed, until at the end of postnatal week 8, all nerves disappeared completely. However, nerves existed in the bladder and posterior urethra permanently. The development and distribution of myenteric nerves is different between the upper and lower part of the urinary tract. Our study aimed to investigate this further.

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