Greenhouse gases Observing SATellite (GOSAT) is the operational satellite dedicated to atmospheric CO 2 observations. Assimilation of data provided by GOSAT is expected to yield reliable CO 2 fluxes in semi-arid regions because of frequent observations owing to clear skies. Here we estimated net CO 2 flux over semi-arid regions of the Southern Hemisphere using the GOSAT column averaged CO 2 (X CO2 ) and surface CO 2 measurements. Assimilation of GOSAT X CO2 indicated that semi-arid regions are integral components of recent terrestrial CO 2 uptake, accounting for 44% globally. Compared with estimates assimilated from surface measurements, estimates by GOSAT X CO2 suggest a 50% reduction in the semi-arid CO 2 uptake, amounting to 1.1 Pg C yr −1 . Significant estimation differences occurred for South America and South Africa, where the GOSAT makes frequent measurements but where surface CO 2 measurements are limited. In comparison, the two estimates varied less in Australia, where more surface measurements are available. These results suggest that GOSAT X CO2 is effective at regulating excess estimates of semi-arid CO 2 uptake in regions that are less constrained by surface CO 2 measurements. To promote understanding of climate change effects in semi-arid regions, it is important to continue monitoring trends in CO 2 uptake with GOSAT.(Citation: Kondo, M., T. Saeki, H. Takagi, K. Ichii, and K. Ishijima, 2016: The effect of GOSAT observations on estimates of net CO 2 flux in semi-arid regions of the Southern Hemisphere.